Q49166 (GYRA_MYCFV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Cleaved into the following chain:

1. Mfl gyrA intein

Gene names

Name:

gyrA

Organism

Mycobacterium flavescens

Taxonomic identifier

1776 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	554 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897
Subunit structure	Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01897.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation By similarity. HAMAP-Rule MF_01897
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Intron homing
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Endonuclease Hydrolase Isomerase Nuclease Topoisomerase
PTM	Autocatalytic cleavage Protein splicing
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 69	›69	DNA gyrase subunit A, 1st part By similarity		PRO_0000034798
	Chain	70 – 490	421	Mfl gyrA intein By similarity		PRO_0000034799
	Chain	491 – ›554	›64	DNA gyrase subunit A, 2nd part By similarity		PRO_0000034800
Regions
	Domain	195 – 335	141	DOD-type homing endonuclease
Sites
	Active site	69	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	554	1

Sequences

	Sequence		Length	Mass (Da)
	Q49166 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: EB8209BBCAAD7765	FASTA	554	60,953
	10 20 30 40 50 60 RGIFRPDRSH AKSARSVAET MGNYHPHGDA SIYDTLVRMA QPWSLRYPLV DGQGNFGSPG 70 80 90 100 110 120 NDPPAAMRYC VTGDALVRLP FGQSVRLRDV VAGARSSSDN AIDLKVLNRH GDPVVADKLF 130 140 150 160 170 180 HSGEHETYTV RTAEGYEVTG TANHPLLCLV DVGGVPTLLW KLTEEIRPGD HVVLQRTPPT 190 200 210 220 230 240 EFGPADWQDA FEALHLGAFI SEGFVSENRA GFNNLDREFF NAVLTAYDTI VGGPRYVSSR 250 260 270 280 290 300 TIASDSLLHE LDVHNLTALK KSRLGELVGQ RSADKAVPEW LWKAPAVVKR VFLQALFEGD 310 320 330 340 350 360 GSCSALPRNT IQVSYSTRSG RLAKDIQQML LEFGVISRRY VHATGEHKVV LTSRAQAELF 370 380 390 400 410 420 AAQIGFGGIK QAKLQGLLDA LPQAAAGRDG DYVPGLAQFV RKHSGSRWVD KDWLNRHNID 430 440 450 460 470 480 RLSRWQRDGA EILGRIADPD VRAIAQELTD GRFYYARVAS VTDSGVQPVY SLRVDTDDHS 490 500 510 520 530 540 FITNGFVSHN TEARLTPLAM EMLREIDEET VDFIPNYDGR VQEPTVLPSR FPNLLANGSG 550 GIAVGMATNI PPHN

References

[1]	"Homing events in the gyrA gene of some mycobacteria." Fsihi H., Vincent V., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 93:3410-3415(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FLA0 / 930991.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z68209 Genomic DNA. Translation: CAA92433.1.
3D structure databases
ProteinModelPortal	Q49166.
SMR	Q49166. Positions 7-69, 71-195, 438-554.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.90.199.10. 2 hits.
HAMAP	MF_01897. GyrA.
InterPro	IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_like_dom. [Graphical view]
Pfam	PF00521. DNA_topoisoIV. 2 hits. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 2 hits.
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRA_MYCFV

Accession

Primary (citable) accession number: Q49166

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program