Reviewed, UniProtKB/Swiss-Prot Q49166 (GYRA_MYCFV)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3
Cleaved into the following chain:
    1- Recommended name:
            Mfl gyrA intein

Gene names

Name:

gyrA

Organism

Mycobacterium flavescens

Taxonomic identifier

1776 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	554 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation By similarity.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Intron homing
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Endonuclease Hydrolase Isomerase Nuclease Topoisomerase
PTM	Autocatalytic cleavage Protein splicing
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 69	›69	DNA gyrase subunit A, 1st part By similarity		PRO_0000034798
	Chain	70 – 490	421	Mfl gyrA intein By similarity		PRO_0000034799
	Chain	491 – ›554	›64	DNA gyrase subunit A, 2nd part By similarity		PRO_0000034800
Regions
	Domain	195 – 335	141	DOD-type homing endonuclease
Sites
	Active site	69	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	554	1

Sequences

	Sequence		Length	Mass (Da)
	Q49166-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: EB8209BBCAAD7765	FASTA	554	60,953
	10 20 30 40 50 60 RGIFRPDRSH AKSARSVAET MGNYHPHGDA SIYDTLVRMA QPWSLRYPLV DGQGNFGSPG 70 80 90 100 110 120 NDPPAAMRYC VTGDALVRLP FGQSVRLRDV VAGARSSSDN AIDLKVLNRH GDPVVADKLF 130 140 150 160 170 180 HSGEHETYTV RTAEGYEVTG TANHPLLCLV DVGGVPTLLW KLTEEIRPGD HVVLQRTPPT 190 200 210 220 230 240 EFGPADWQDA FEALHLGAFI SEGFVSENRA GFNNLDREFF NAVLTAYDTI VGGPRYVSSR 250 260 270 280 290 300 TIASDSLLHE LDVHNLTALK KSRLGELVGQ RSADKAVPEW LWKAPAVVKR VFLQALFEGD 310 320 330 340 350 360 GSCSALPRNT IQVSYSTRSG RLAKDIQQML LEFGVISRRY VHATGEHKVV LTSRAQAELF 370 380 390 400 410 420 AAQIGFGGIK QAKLQGLLDA LPQAAAGRDG DYVPGLAQFV RKHSGSRWVD KDWLNRHNID 430 440 450 460 470 480 RLSRWQRDGA EILGRIADPD VRAIAQELTD GRFYYARVAS VTDSGVQPVY SLRVDTDDHS 490 500 510 520 530 540 FITNGFVSHN TEARLTPLAM EMLREIDEET VDFIPNYDGR VQEPTVLPSR FPNLLANGSG 550 GIAVGMATNI PPHN

References

[1]	"Homing events in the gyrA gene of some mycobacteria." Fsihi H., Vincent V., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 93:3410-3415(1996) [PubMed: 8622949] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FLA0 / 930991.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z68209 Genomic DNA. Translation: CAA92433.1.
3D structure databases
HSSP	HSSP built from PDB template 1AM2 based on UniProtKB P72065.
SMR	Q49166. Positions 71-195, 438-490.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.99.1.3. 306541.
Family and domain databases
InterPro	IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit.
Pfam	PF00521. DNA_topoisoIV. 2 hits. [Graphical view]
PRINTS	PR00379. INTEIN.
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRA_MYCFV

Accession

Primary (citable) accession number: Q49166

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	July 28, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)