Q49158 (SYK_MYCFP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysine--tRNA ligase
EC=6.1.1.6

Alternative name(s):
Lysyl-tRNA synthetase
Short name=LysRS

Gene names

Name:	lysS
Ordered Locus Names:	MBIO_0660

Organism

Mycoplasma fermentans (strain ATCC 19989 / NBRC 14854 / NCTC 10117 / PG18) [Complete proteome] [HAMAP]

Taxonomic identifier

496833 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma fermentans

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP-Rule MF_00252
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.
Sequence caution	The sequence BAH69925.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Lysine--tRNA ligase HAMAP-Rule MF_00252		PRO_0000152647
Sites
	Metal binding	397	1	Magnesium 1 By similarity
	Metal binding	404	1	Magnesium 1 By similarity
	Metal binding	404	1	Magnesium 2 By similarity
Experimental info
	Sequence conflict	2	1	T → A in AAC43988. Ref.1
	Sequence conflict	8	1	E → D in AAC43988. Ref.1
	Sequence conflict	120	1	G → C in AAC43988. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q49158 [UniParc]. Last modified November 28, 2012. Version 2. Checksum: A0BDF2B1FF794025	FASTA	488	56,701
	10 20 30 40 50 60 MTKYTEQEQI RRDKLEFYKK FDVVPFKKAY GLGKLSTSDE LNKKYGQFAR EELEAKKVKK 70 80 90 100 110 120 NISGRLITAR GPFLVLKDRK GTIQVYFNKK ENPELTKIVE TFDLGDILWV RGLLMKTHTG 130 140 150 160 170 180 EMTVRAQDIQ LLTKALKPLP DKFHGLTDTE ERYRHRYLDL ITNPESRNTF IMRTKIVQWI 190 200 210 220 230 240 RDYFNKLDYL EAETPFLHDY LSGAAAKPFT THHNSLNQDF VLRIATEIPL KKLVVGGFER 250 260 270 280 290 300 VYEMGRIFRN EGYDTTHNPE FTTIEFYEAY SNVEGMMNRT ETLIKELCKK IGKSKFVTNG 310 320 330 340 350 360 VEVDLSKPFK RVNMIDEVSK KTGKNFRKIT LEEAIEVAKA YKIKIEKFFT IGHIINALYE 370 380 390 400 410 420 ELVEPTLIQP TFLYGHPIEI SPLSAKSDDP RFTERAELFI NTKEYANMYT ELSDPIDQLE 430 440 450 460 470 480 RFESQLEEKN KGNDEASDID YDFVDALEYG MPPTGGCGIG IDRLVMLLTE TDSIRDVLLF PTLKRIKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Antigenic topology of the P29 surface lipoprotein of Mycoplasma fermentans: differential display of epitopes results in high-frequency phase variation." Theiss P., Karpas A., Wise K. Infect. Immun. 64:1800-1809(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 19989 / NBRC 14854 / NCTC 10117 / PG18.
[2]	"Molecular cloning and expression of a novel cholinephosphotransferase involved in glycoglycerophospholipid biosynthesis of Mycoplasma fermentans." Ishida N., Irikura D., Matsuda K., Sato S., Sone T., Tanaka M., Asano K. Curr. Microbiol. 58:535-540(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19989 / NBRC 14854 / NCTC 10117 / PG18.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U50826 Genomic DNA. Translation: AAC43988.1. AP009608 Genomic DNA. Translation: BAH69925.1. Different initiation.
3D structure databases
ProteinModelPortal	Q49158.
ModBase	Search...
Proteomic databases
PRIDE	Q49158.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAH69925; BAH69925; MBIO_0660.
PATRIC	43128069. VBIMycFer131377_0252.
Organism-specific databases
CMR	Search...
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
HAMAP	MF_00252. Lys_tRNA_synth_class2.
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR002313. Lys-tRNA-ligase_II. IPR018149. Lys-tRNA-synth_II_C. IPR012340. NA-bd_OB-fold. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
PANTHER	PTHR22594. PTHR22594. 1 hit. PTHR22594:SF4. PTHR22594:SF4. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00499. lysS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK_MYCFP

Accession

Primary (citable) accession number: Q49158
Secondary accession number(s): C4XFK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 28, 2012
Last modified:	April 3, 2013
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program