Q49150 (PQQCD_METEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional coenzyme PQQ synthesis protein C/D

Alternative name(s):
Pyrroloquinoline quinone biosynthesis protein C/D

Including the following 2 domains:

1. Pyrroloquinoline-quinone synthase
   EC=1.3.3.11
   Alternative name(s):
   Coenzyme PQQ synthesis protein C
2. Coenzyme PQQ synthesis protein D

Gene names

Name:	pqqCD
Ordered Locus Names:	MexAM1_META1p1749

Organism

Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1) [Complete proteome] [HAMAP]

Taxonomic identifier

272630 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic-acid to PQQ By similarity. HAMAP MF_00654
Catalytic activity	6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,7,8-hexahydroquinoline-2,4-dicarboxylate + 3 O2 = 4,5-dioxo-4,5-dihydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + 2 H2O2 + 2 H2O. HAMAP MF_00654
Pathway	Cofactor biosynthesis; pyrroloquinoline quinone biosynthesis. HAMAP MF_00654
Sequence similarities	In the N-terminal section; belongs to the pqqC family. In the C-terminal section; belongs to the pqqD family.

Ontologies

Keywords
Biological process	PQQ biosynthesis
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrroloquinoline quinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	pyrroloquinoline-quinone synthase activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Bifunctional coenzyme PQQ synthesis protein C/D HAMAP MF_00654		PRO_0000219980
Regions
	Region	1 – 267	267	PqqC HAMAP MF_00654
	Region	268 – 280	13	Linker HAMAP MF_00654
	Region	281 – 372	92	PqqD HAMAP MF_00654

Sequences

	Sequence		Length	Mass (Da)
	Q49150 [UniParc]. Last modified June 16, 2003. Version 2. Checksum: 3E502CEB4097695D	FASTA	372	41,577
	10 20 30 40 50 60 MTAQFPPPVP DTEQRLLSHE ELEAALRDIG ARRYHNLHPF HRLLHDGKLS KDQVRAWALN 70 80 90 100 110 120 RYYYQAMIPV KDAALLARLP DAQLRRIWRQ RIVDHDGDHE GDGGIERWLK LAEGVGFTRD 130 140 150 160 170 180 YVLSTKGILS ATRFSVDAYV HFVSERSLLE AIASSLTEMF SPTIISERVA GMLKNYDFIT 190 200 210 220 230 240 KDTLAYFDKR LTQAPRDADF ALDYVKRHAT TPEMQRAAID ALTFKCNVLW TQLDALYFAY 250 260 270 280 290 300 VAPGMVPPDA WQPGEGLVAE TNSAEDSPAA AASPAATTAE PTAFSGSDVP RLPRGVRLRF 310 320 330 340 350 360 DEVRNKHVLL APERTFDLDD NAVAVLKLVD GRNTVSQIAQ ILGQTYDADP AIIEADILPM 370 LAGLAQKRVL ER

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources." Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y., Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W., Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E. , Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D., Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J., Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C., Lidstrom M.E. PLoS ONE 4:E5584-E5584(2009) [PubMed: 19440302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14718 / DSM 1338 / AM1.
[2]	"Isolation, phenotypic characterization, and complementation analysis of mutants of Methylobacterium extorquens AM1 unable to synthesize pyrroloquinoline quinone and sequences of pqqD, pqqG, and pqqC." Morris C.J., Biville F., Turlin E., Lee E., Ellermann K., Fan W.H., Ramamoorthi R., Springer A.L., Lidstrom M.E. J. Bacteriol. 176:1746-1755(1994) [PubMed: 8132470] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-111.
[3]	"Sequence analysis of pqq genes required for biosynthesis of pyrroloquinoline quinone in Methylobacterium extorquens AM1 and the purification of a biosynthetic intermediate." Toyama H., Chistoserdova L., Lidstrom M.E. Microbiology 143:595-602(1997) [PubMed: 9043136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 110-372.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001510 Genomic DNA. Translation: ACS39593.1. L25889 Unassigned DNA. Translation: AAA17880.1. U72662 Genomic DNA. Translation: AAB58899.1.
PIR	C55527.
RefSeq	YP_002962870.1. NC_012808.1.
3D structure databases
ProteinModelPortal	Q49150.
ModBase	Search...
Protein-protein interaction databases
STRING	Q49150.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7991712.
PATRIC	22509330. VBIMetExt101010_1740.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FRDRATR.
ProtClustDB	PRK05157.
Enzyme and pathway databases
BRENDA	1.3.3.11. 3296.
Family and domain databases
HAMAP	MF_00654. PQQ_syn_PqqC. Fused. [Tree] MF_00655. PQQ_syn_PqqD. Fused. [Tree]
InterPro	IPR016084. Haem_Oase-like_multi-hlx. IPR011845. PQQ_synth_PqqC. IPR008792. PQQ_synth_PqqD. IPR022479. PQQ_synth_PqqD_bac. IPR004305. TENA/THI4/PQQ_synthPqqC_dom. [Graphical view]
Gene3D	G3DSA:1.20.910.10. Haem_Oase-like_multi-hlx. 1 hit.
Pfam	PF05402. PqqD. 1 hit. PF03070. TENA_THI-4. 1 hit. [Graphical view]
SUPFAM	SSF48613. Heme_oxygenase. 1 hit.
TIGRFAMs	TIGR03859. PQQ_PqqD. 1 hit. TIGR02111. PQQ_syn_pqqC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PQQCD_METEA

Accession

Primary (citable) accession number: Q49150
Secondary accession number(s): C5B129, P71518

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	June 16, 2003
Last modified:	January 25, 2012
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program