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Reviewed, UniProtKB/Swiss-Prot Q49150 (PQQCD_METEA)

Last modified February 9, 2010. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Bifunctional coenzyme PQQ synthesis protein C/D
Alternative name(s):
    Pyrroloquinoline quinone biosynthesis protein C/D
Including the following 2 domains:
    1- Recommended name:
            Pyrroloquinoline-quinone synthase
              EC=1.3.3.11
        Alternative name(s):
            Coenzyme PQQ synthesis protein C
    2- Recommended name:
            Coenzyme PQQ synthesis protein D
Gene names
Name: pqqCD
Ordered Locus Names: MexAM1_META1p1749
OrganismMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1) [Complete proteome] [HAMAP]
Taxonomic identifier272630 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic-acid to PQQ By similarity. HAMAP MF_00654

Catalytic activity

6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,7,8-hexahydroquinoline-2,4-dicarboxylate + 3 O2 = 4,5-dioxo-4,5-dihydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + 2 H2O2 + 2 H2O. HAMAP MF_00654

Pathway

Cofactor biosynthesis; pyrroloquinoline quinone biosynthesis. HAMAP MF_00654

Sequence similarities

In the N-terminal section; belongs to the pqqC family.

In the C-terminal section; belongs to the pqqD family.

Ontologies

Keywords
   Biological processPQQ biosynthesis
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

pyrroloquinoline quinone biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Molecular functionpyrroloquinoline-quinone synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Bifunctional coenzyme PQQ synthesis protein C/D HAMAP MF_00654
PRO_0000219980

Regions

Region1 – 267267PqqC HAMAP MF_00654
Region268 – 28013Linker HAMAP MF_00654
Region281 – 37292PqqD HAMAP MF_00654

Sequences

Sequence LengthMass (Da)Tools
Q49150-1 [UniParc].

Last modified June 16, 2003. Version 2.
Checksum: 3E502CEB4097695D

FASTA37241,577
        10         20         30         40         50         60 
MTAQFPPPVP DTEQRLLSHE ELEAALRDIG ARRYHNLHPF HRLLHDGKLS KDQVRAWALN 

        70         80         90        100        110        120 
RYYYQAMIPV KDAALLARLP DAQLRRIWRQ RIVDHDGDHE GDGGIERWLK LAEGVGFTRD 

       130        140        150        160        170        180 
YVLSTKGILS ATRFSVDAYV HFVSERSLLE AIASSLTEMF SPTIISERVA GMLKNYDFIT 

       190        200        210        220        230        240 
KDTLAYFDKR LTQAPRDADF ALDYVKRHAT TPEMQRAAID ALTFKCNVLW TQLDALYFAY 

       250        260        270        280        290        300 
VAPGMVPPDA WQPGEGLVAE TNSAEDSPAA AASPAATTAE PTAFSGSDVP RLPRGVRLRF 

       310        320        330        340        350        360 
DEVRNKHVLL APERTFDLDD NAVAVLKLVD GRNTVSQIAQ ILGQTYDADP AIIEADILPM 

       370 
LAGLAQKRVL ER 

« Hide

References

« Hide 'large scale' references
[1]"Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources."
Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y., Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W., Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E. expand/collapse author list , Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D., Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J., Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C., Lidstrom M.E.
PLoS ONE 4:E5584-E5584(2009) [PubMed: 19440302] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Isolation, phenotypic characterization, and complementation analysis of mutants of Methylobacterium extorquens AM1 unable to synthesize pyrroloquinoline quinone and sequences of pqqD, pqqG, and pqqC."
Morris C.J., Biville F., Turlin E., Lee E., Ellermann K., Fan W.H., Ramamoorthi R., Springer A.L., Lidstrom M.E.
J. Bacteriol. 176:1746-1755(1994) [PubMed: 8132470] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-111.
[3]"Sequence analysis of pqq genes required for biosynthesis of pyrroloquinoline quinone in Methylobacterium extorquens AM1 and the purification of a biosynthetic intermediate."
Toyama H., Chistoserdova L., Lidstrom M.E.
Microbiology 143:595-602(1997) [PubMed: 9043136] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 110-372.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001510 Genomic DNA. Translation: ACS39593.1.
L25889 Unassigned DNA. Translation: AAA17880.1.
U72662 Genomic DNA. Translation: AAB58899.1.
PIRC55527.
RefSeqYP_002962870.1.

3D structure databases

SMRQ49150. Positions 15-248.
ModBaseSearch...

Genome annotation databases

GeneID7991712.

Organism-specific databases

CMRSearch...

Enzyme and pathway databases

BRENDA1.3.3.11. 20440.

Family and domain databases

HAMAPMF_00654. PQQ_syn_PqqC. Fused.
[Tree]
MF_00655. PQQ_syn_PqqD. Fused.
[Tree]
InterProIPR016084. Haem_Oase-like_multi-hlx.
IPR011845. PQQ_synth_PqqC.
IPR004305. TENA/THI4/PQQ_synth_PqqC.
[Graphical view]
Gene3DG3DSA:1.20.910.10. Haem_Oase-like_multi-hlx. 1 hit.
PfamPF03070. TENA_THI-4. 1 hit.
[Graphical view]
TIGRFAMsTIGR02111. PQQ_syn_pqqC. 1 hit.
ProtoNetSearch...

Entry information

Entry namePQQCD_METEA
AccessionPrimary (citable) accession number: Q49150
Secondary accession number(s): C5B129, P71518
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: February 9, 2010
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents