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Q49136 (CAPP_METEA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxylase

Short name=PEPC
Short name=PEPCase
EC=4.1.1.31
Gene names
Name:ppc
Synonyms:ppcA
Ordered Locus Names:MexAM1_META1p1732
OrganismMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1) [Complete proteome] [HAMAP]
Taxonomic identifier272630 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length922 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle By similarity.

Catalytic activity

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.

Cofactor

Magnesium By similarity.

Sequence similarities

Belongs to the PEPCase type 1 family.

Ontologies

Keywords
   Biological processCarbon dioxide fixation
   LigandMagnesium
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcarbon fixation

Inferred from electronic annotation. Source: UniProtKB-KW

tricarboxylic acid cycle

Inferred from electronic annotation. Source: InterPro

   Molecular_functionphosphoenolpyruvate carboxylase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 922922Phosphoenolpyruvate carboxylase
PRO_0000166599

Sites

Active site1421 By similarity
Active site5811 By similarity

Experimental info

Sequence conflict281E → A in AAA62656. Ref.3
Sequence conflict5061D → E in AAB58883. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q49136 [UniParc].

Last modified September 22, 2009. Version 2.
Checksum: 11B90AD09BB5683E

FASTA922103,021
        10         20         30         40         50         60 
MTKTLHARPS AATDTTFAPP VITGTATEDA LEILFHALLD VARRHDPELE DVLHGRADIS 

        70         80         90        100        110        120 
SFTPEMLARA LQVQGIWFQL VSIAEQNAAM RRRRHVERDQ GREALNGSFA KVLAEASARG 

       130        140        150        160        170        180 
IGPQQIHALL KDLRIRPTIT AHPTEGKRVT VLEKLRRIYL VLRELELPRW TERERNGLMN 

       190        200        210        220        230        240 
ELRDQIELIW MTGELHLEKA TVEREVAWGL HFFDETLFEM LPEMLLSLEE SLAQYYPDET 

       250        260        270        280        290        300 
FEVPPFFQFG SWIGGDRDGN PYVTASVTRE TLQRNALASL RRYRDGITHL GRVLSITERS 

       310        320        330        340        350        360 
LPVPETFRSE LAHMLAESGD ARAIANRNPG EAYRQFLSCV LRKLEATIAR NKGARSVGPD 

       370        380        390        400        410        420 
YPSADGLIND LRTLEKGLAD AKCGALATDI VRPVRRMVEI FRFSTVRLDL RENSTRTTKT 

       430        440        450        460        470        480 
LHALWKLRNG DREPPALDSP AWKDWLLTEL ARPRTPETSF EDFADRLPDD ARETLATFAL 

       490        500        510        520        530        540 
VGEMRDTLDR EAFGAFILSM TRSTVDVLGA YLLAKEAGIF LDTTGTEICP LPIVPLFETI 

       550        560        570        580        590        600 
DDLRAAPAIM KELLGIPVVR RSTRWQGGVQ EVMIGYSDSN KDGGFIASNW ELYKAQVRLT 

       610        620        630        640        650        660 
TLGNHLGVPI AFFHGRGGSV SRGGVPTHRG IAAQPPGSIQ GRFRITEQGE VVSFKYANRG 

       670        680        690        700        710        720 
TAAYQMELLA ASVFEHALLS EGNGNGSRAE FDDALEALSG ASRAAYVNLL QAEGLVDYFQ 

       730        740        750        760        770        780 
AASPLDEISL LNIGSRPARR FGAKSLSDLR AIPWVFAWSQ NRHVITGWYG VGSGLKSFID 

       790        800        810        820        830        840 
VRGEAGEALL RRLFRDCRVF RLVLDEVEKT LLMVDLEIAR DYAGLVEDAG IRARIFGMIE 

       850        860        870        880        890        900 
AEYALTREMV LRVSGDSELA QRFPQFSERL RGRLPTINQV SREQVELLRR YRSETDEDKR 

       910        920 
EAVKSALLLS INCIAVGFGA TG 

« Hide

References

« Hide 'large scale' references
[1]"Molecular and mutational analysis of a DNA region separating two methylotrophy gene clusters in Methylobacterium extorquens AM1."
Chistoserdova L.V., Lidstrom M.E.
Microbiology 143:1729-1736(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources."
Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y., Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W., Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E. expand/collapse author list , Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D., Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J., Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C., Lidstrom M.E.
PLoS ONE 4:E5584-E5584(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 14718 / DSM 1338 / AM1.
[3]"Genetics of the serine cycle in Methylobacterium extorquens AM1: identification, sequence, and mutation of three new genes involved in C1 assimilation, orf4, mtkA, and mtkB."
Chistoserdova L.V., Lidstrom M.E.
J. Bacteriol. 176:7398-7404(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U72662 Genomic DNA. Translation: AAB58883.2.
CP001510 Genomic DNA. Translation: ACS39576.1.
L33465 Genomic DNA. Translation: AAA62656.1.
PIRD55230.
RefSeqYP_002962853.1. NC_012808.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACS39576; ACS39576; MexAM1_META1p1732.
GeneID7991695.
KEGGmea:Mex_1p1732.
PATRIC22509294. VBIMetExt101010_1722.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2352.
HOGENOMHOG000238647.
KOK01595.
OMAISCQHYR.
OrthoDBEOG6TJ7T8.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-3824.
MEXT272630:GBY6-1656-MONOMER.

Family and domain databases

InterProIPR021135. PEP_COase.
IPR018129. PEP_COase_AS.
IPR022805. PEP_COase_bac/pln-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamPF00311. PEPcase. 1 hit.
[Graphical view]
PRINTSPR00150. PEPCARBXLASE.
SUPFAMSSF51621. SSF51621. 1 hit.
PROSITEPS00393. PEPCASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCAPP_METEA
AccessionPrimary (citable) accession number: Q49136
Secondary accession number(s): C5B112, P71502
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 22, 2009
Last modified: May 14, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families