ID   FCHA_METEX              Reviewed;         208 AA.
AC   Q49135;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   16-JUN-2009, entry version 45.
DE   RecName: Full=Methenyltetrahydrofolate cyclohydrolase;
DE            EC=3.5.4.9;
GN   Name=fchA;
OS   Methylobacterium extorquens (Protomonas extorquens).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=408;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC   STRAIN=AM1 / NCIMB 9133;
RX   MEDLINE=95050329; PubMed=7961516;
RA   Chistoserdova L.V., Lidstrom M.E.;
RT   "Genetics of the serine cycle in Methylobacterium extorquens AM1:
RT   identification, sequence, and mutation of three new genes involved in
RT   C1 assimilation, orf4, mtkA, and mtkB.";
RL   J. Bacteriol. 176:7398-7404(1994).
RN   [2]
RP   CHARACTERIZATION, AND PROTEIN SEQUENCE OF 1-21.
RC   STRAIN=AM1 / NCIMB 9133;
RX   MEDLINE=99234022; PubMed=10215859;
RX   DOI=10.1046/j.1432-1327.1999.00291.x;
RA   Pomper B.K., Vorholt J.A., Chistoserdova L.V., Lidstrom M.E.,
RA   Thauer R.K.;
RT   "A methenyl tetrahydromethanopterin cyclohydrolase and a methenyl
RT   tetrahydrofolate cyclohydrolase in Methylobacterium extorquens AM1.";
RL   Eur. J. Biochem. 261:475-480(1999).
CC   -!- FUNCTION: Required for both C1 and C2 metabolism.
CC   -!- CATALYTIC ACTIVITY: 5,10-methenyltetrahydrofolate + H(2)O = 10-
CC       formyltetrahydrofolate.
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde assimilation via
CC       serine pathway.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein
CC       (Potential).
CC   -!- DISRUPTION PHENOTYPE: Mutants are unable to grow on either
CC       methanol or ethylamine in the presence of glycolate. Glyoxylate
CC       restores growth, but only on C2 compounds.
CC   -!- SIMILARITY: Belongs to the cyclodeaminase/cyclohydrolase family.
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DR   EMBL; L33465; AAA62653.1; -; Genomic_DNA.
DR   PIR; A55230; A55230.
DR   BioCyc; MetaCyc:MON-3943; -.
DR   BRENDA; 3.5.4.9; 20440.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004477; F:methenyltetrahydrofolate cyclohydrolase act...; IEA:EC.
DR   GO; GO:0006730; P:one-carbon compound metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR007044; Cyclodeamin/CycHdrlase.
DR   Pfam; PF04961; FTCD_C; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Membrane; One-carbon metabolism;
KW   Transmembrane.
FT   CHAIN         1    208       Methenyltetrahydrofolate cyclohydrolase.
FT                                /FTId=PRO_0000087208.
FT   TRANSMEM     25     46       Potential.
SQ   SEQUENCE   208 AA;  21726 MW;  04696128ECDD075B CRC64;
     MAGNETIETF LDGLASSAPT PGGGGAAAIS GAMGAALVSM VCNLTIGKKK YVEVEADLMQ
     VLEKSEGLRR TLTGMIADDV EAFDAVMGAY GLPKNTDEEK AARAAKIQEA LKTATDVPLA
     CCRVCREVID LAEIVAEKGN LNVISDAGVA VLSAYAGLRS AALNVYVNAK GLDDRAFAEE
     RLKELEGLLA EAGALNERIY ETVKSKVN
//