Reviewed, UniProtKB/Swiss-Prot Q49135 (FCHA_METEX)

Names and origin

Protein names

Recommended name:
    Methenyltetrahydrofolate cyclohydrolase
    EC=3.5.4.9

Gene names

Name:

fchA

Organism

Methylobacterium extorquens (Protomonas extorquens)

Taxonomic identifier

408 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for both C1 and C2 metabolism.
Catalytic activity	5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.
Pathway	One-carbon metabolism; formaldehyde assimilation via serine pathway.
Subunit structure	Homodimer.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Disruption phenotype	Mutants are unable to grow on either methanol or ethylamine in the presence of glycolate. Glyoxylate restores growth, but only on C2 compounds. Ref.1
Sequence similarities	Belongs to the cyclodeaminase/cyclohydrolase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methenyltetrahydrofolate cyclohydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Methenyltetrahydrofolate cyclohydrolase		PRO_0000087208
Regions
	Transmembrane	25 – 46	22	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q49135-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 04696128ECDD075B	FASTA	208	21,726
	10 20 30 40 50 60 MAGNETIETF LDGLASSAPT PGGGGAAAIS GAMGAALVSM VCNLTIGKKK YVEVEADLMQ 70 80 90 100 110 120 VLEKSEGLRR TLTGMIADDV EAFDAVMGAY GLPKNTDEEK AARAAKIQEA LKTATDVPLA 130 140 150 160 170 180 CCRVCREVID LAEIVAEKGN LNVISDAGVA VLSAYAGLRS AALNVYVNAK GLDDRAFAEE 190 200 RLKELEGLLA EAGALNERIY ETVKSKVN

References

[1]	"Genetics of the serine cycle in Methylobacterium extorquens AM1: identification, sequence, and mutation of three new genes involved in C1 assimilation, orf4, mtkA, and mtkB." Chistoserdova L.V., Lidstrom M.E. J. Bacteriol. 176:7398-7404(1994) [PubMed: 7961516] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE. Strain: AM1 / NCIMB 9133.
[2]	"A methenyl tetrahydromethanopterin cyclohydrolase and a methenyl tetrahydrofolate cyclohydrolase in Methylobacterium extorquens AM1." Pomper B.K., Vorholt J.A., Chistoserdova L.V., Lidstrom M.E., Thauer R.K. Eur. J. Biochem. 261:475-480(1999) [PubMed: 10215859] [Abstract] Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 1-21. Strain: AM1 / NCIMB 9133.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L33465 Genomic DNA. Translation: AAA62653.1.
PIR	A55230.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-3943.
BRENDA	3.5.4.9. 20440.
Family and domain databases
InterPro	IPR007044. Cyclodeamin/CycHdrlase. [Graphical view]
Pfam	PF04961. FTCD_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FCHA_METEX

Accession

Primary (citable) accession number: Q49135

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)