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Q49118

- FHCD_METEA

UniProt

Q49118 - FHCD_METEA

Protein

Formyltransferase/hydrolase complex subunit D

Gene

fhcD

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in the transformation of 5-formyl tetrahydromethanopterin (5-formyl-H4MPT) to methanofuran (MFR) and formate via the intermediate formylmethanofuran (formyl-MFR). Catalyzes the transfer of a formyl group from 5-formyl-H4MPT to MFR so as to produce tetrahydromethanopterin (H4MPT) and formyl-MFR, which is then hydrolyzed to formate and MFR.2 Publications

    Catalytic activityi

    Formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin.2 Publications

    Kineticsi

    1. KM=30 µM for H4MPT1 Publication
    2. KM=50 µM for formyl-MFR1 Publication

    Vmax=80 µmol/min/mg enzyme1 Publication

    pH dependencei

    Optimum pH is 7.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. formylmethanofuran-tetrahydromethanopterin N-formyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. formaldehyde catabolic process Source: UniProtKB-UniPathway
    2. one-carbon metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    One-carbon metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-4065.
    MEXT272630:GBY6-1680-MONOMER.
    UniPathwayiUPA00562; UER00704.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Formyltransferase/hydrolase complex subunit D (EC:2.3.1.101)
    Short name:
    Ftr complex
    Alternative name(s):
    Formylmethanofuran--tetrahydromethanopterin formyltransferase
    H4MPT formyltransferase
    Gene namesi
    Name:fhcD
    Synonyms:ffsA
    Ordered Locus Names:MexAM1_META1p1756
    OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
    Taxonomic identifieri272630 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
    ProteomesiUP000009081: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 311310Formyltransferase/hydrolase complex subunit DPRO_0000138124Add
    BLAST

    Interactioni

    Subunit structurei

    Octaheteromer. Part of the formyltransferase/hydrolase complex fhc; composed of FhcA, FhcB, FhcC and FhcD.2 Publications

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FTR family.Curated

    Phylogenomic databases

    eggNOGiCOG2037.
    HOGENOMiHOG000230959.
    KOiK00672.
    OMAiLERIGQC.
    OrthoDBiEOG664CDN.

    Family and domain databases

    Gene3Di3.30.70.520. 2 hits.
    HAMAPiMF_00579. FTR.
    InterProiIPR014053. ForMFR_H4MPT_ForTrfase.
    IPR002770. ForMFR_H4MPT_ForTrfase_C.
    IPR023447. ForMFR_H4MPT_ForTrfase_fd-like.
    IPR022667. ForMFR_H4MPT_ForTrfase_N.
    [Graphical view]
    PfamiPF01913. FTR. 1 hit.
    PF02741. FTR_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006414. Ftr_formyl_trnsf. 1 hit.
    SUPFAMiSSF55112. SSF55112. 2 hits.
    TIGRFAMsiTIGR03119. one_C_fhcD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q49118-1 [UniParc]FASTAAdd to Basket

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    MSDFTLNGIK VEDTFAEAFD VAGTAIIVTN DTPKWAMIAA TVMTGFATSV    50
    IGCGAEAGID AELSPDETPD GRPGVRILLF GFEPNGLKDQ LLKRVGQCIL 100
    TCPGTACFAG VEGPTKIKLG GAIRYFGDGF AVAKRLPDHE GKMRRYWRIP 150
    VMDGEFLCED SVRAVDGAVG GGNLLFLGRK HADTLIVAEI AVEAAKAIPG 200
    AILPFPGGIV RSGSKVGGRT KGMMASTNDA YCPTLKGRAG SALPPECGVV 250
    LEIVIDALTS AAVAESMRAA LHAATEIGAQ HGLVAVTAGN YGGNLGRHHY 300
    HLRDLLEKPA A 311
    Length:311
    Mass (Da):32,374
    Last modified:January 23, 2007 - v4
    Checksum:iF25FBE3918729428
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF032114 Genomic DNA. Translation: AAC26972.1.
    CP001510 Genomic DNA. Translation: ACS39600.1.
    PIRiT45153.
    RefSeqiYP_002962877.1. NC_012808.1.

    Genome annotation databases

    EnsemblBacteriaiACS39600; ACS39600; MexAM1_META1p1756.
    GeneIDi7991719.
    KEGGimea:Mex_1p1756.
    PATRICi22509342. VBIMetExt101010_1746.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF032114 Genomic DNA. Translation: AAC26972.1 .
    CP001510 Genomic DNA. Translation: ACS39600.1 .
    PIRi T45153.
    RefSeqi YP_002962877.1. NC_012808.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACS39600 ; ACS39600 ; MexAM1_META1p1756 .
    GeneIDi 7991719.
    KEGGi mea:Mex_1p1756.
    PATRICi 22509342. VBIMetExt101010_1746.

    Phylogenomic databases

    eggNOGi COG2037.
    HOGENOMi HOG000230959.
    KOi K00672.
    OMAi LERIGQC.
    OrthoDBi EOG664CDN.

    Enzyme and pathway databases

    UniPathwayi UPA00562 ; UER00704 .
    BioCyci MetaCyc:MONOMER-4065.
    MEXT272630:GBY6-1680-MONOMER.

    Family and domain databases

    Gene3Di 3.30.70.520. 2 hits.
    HAMAPi MF_00579. FTR.
    InterProi IPR014053. ForMFR_H4MPT_ForTrfase.
    IPR002770. ForMFR_H4MPT_ForTrfase_C.
    IPR023447. ForMFR_H4MPT_ForTrfase_fd-like.
    IPR022667. ForMFR_H4MPT_ForTrfase_N.
    [Graphical view ]
    Pfami PF01913. FTR. 1 hit.
    PF02741. FTR_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006414. Ftr_formyl_trnsf. 1 hit.
    SUPFAMi SSF55112. SSF55112. 2 hits.
    TIGRFAMsi TIGR03119. one_C_fhcD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "C1 transfer enzymes and coenzymes linking methylotrophic bacteria and methanogenic Archaea."
      Chistoserdova L.V., Vorholt J.A., Thauer R.K., Lidstrom M.E.
      Science 281:99-102(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 14718 / DSM 1338 / AM1.
    3. "Characterization of the formyltransferase from Methylobacterium extorquens AM1."
      Pomper B.K., Vorholt J.A.
      Eur. J. Biochem. 268:4769-4775(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-16, FUNCTION AS A FORMYLTRANSFERASE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
    4. "Generation of formate by the formyltransferase/hydrolase complex (Fhc) from Methylobacterium extorquens AM1."
      Pomper B.K., Saurel O., Milon A., Vorholt J.A.
      FEBS Lett. 523:133-137(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.

    Entry informationi

    Entry nameiFHCD_METEA
    AccessioniPrimary (citable) accession number: Q49118
    Secondary accession number(s): C5B136
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 10, 2002
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 98 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3