Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Formyltransferase/hydrolase complex subunit D

Gene

fhcD

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transformation of 5-formyl tetrahydromethanopterin (5-formyl-H4MPT) to methanofuran (MFR) and formate via the intermediate formylmethanofuran (formyl-MFR). Catalyzes the transfer of a formyl group from 5-formyl-H4MPT to MFR so as to produce tetrahydromethanopterin (H4MPT) and formyl-MFR, which is then hydrolyzed to formate and MFR.2 Publications

Catalytic activityi

Formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin.2 Publications

Kineticsi

  1. KM=30 µM for H4MPT1 Publication
  2. KM=50 µM for formyl-MFR1 Publication

Vmax=80 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 7.1 Publication

Pathwayi

GO - Molecular functioni

  1. formylmethanofuran-tetrahydromethanopterin N-formyltransferase activity Source: UniProtKB

GO - Biological processi

  1. formaldehyde catabolic process Source: UniProtKB-UniPathway
  2. one-carbon metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

One-carbon metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-4065.
MEXT272630:GBY6-1680-MONOMER.
UniPathwayiUPA00562; UER00704.

Names & Taxonomyi

Protein namesi
Recommended name:
Formyltransferase/hydrolase complex subunit D (EC:2.3.1.101)
Short name:
Ftr complex
Alternative name(s):
Formylmethanofuran--tetrahydromethanopterin formyltransferase
H4MPT formyltransferase
Gene namesi
Name:fhcD
Synonyms:ffsA
Ordered Locus Names:MexAM1_META1p1756
OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Taxonomic identifieri272630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000009081 Componenti: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 311310Formyltransferase/hydrolase complex subunit DPRO_0000138124Add
BLAST

Interactioni

Subunit structurei

Octaheteromer. Part of the formyltransferase/hydrolase complex fhc; composed of FhcA, FhcB, FhcC and FhcD.2 Publications

Family & Domainsi

Sequence similaritiesi

Belongs to the FTR family.Curated

Phylogenomic databases

eggNOGiCOG2037.
HOGENOMiHOG000230959.
KOiK00672.
OMAiCPAEAGI.
OrthoDBiEOG664CDN.

Family and domain databases

Gene3Di3.30.70.520. 2 hits.
HAMAPiMF_00579. FTR.
InterProiIPR014053. ForMFR_H4MPT_ForTrfase.
IPR002770. ForMFR_H4MPT_ForTrfase_C.
IPR023447. ForMFR_H4MPT_ForTrfase_fd-like.
IPR022667. ForMFR_H4MPT_ForTrfase_N.
[Graphical view]
PfamiPF01913. FTR. 1 hit.
PF02741. FTR_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006414. Ftr_formyl_trnsf. 1 hit.
SUPFAMiSSF55112. SSF55112. 2 hits.
TIGRFAMsiTIGR03119. one_C_fhcD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q49118-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDFTLNGIK VEDTFAEAFD VAGTAIIVTN DTPKWAMIAA TVMTGFATSV
60 70 80 90 100
IGCGAEAGID AELSPDETPD GRPGVRILLF GFEPNGLKDQ LLKRVGQCIL
110 120 130 140 150
TCPGTACFAG VEGPTKIKLG GAIRYFGDGF AVAKRLPDHE GKMRRYWRIP
160 170 180 190 200
VMDGEFLCED SVRAVDGAVG GGNLLFLGRK HADTLIVAEI AVEAAKAIPG
210 220 230 240 250
AILPFPGGIV RSGSKVGGRT KGMMASTNDA YCPTLKGRAG SALPPECGVV
260 270 280 290 300
LEIVIDALTS AAVAESMRAA LHAATEIGAQ HGLVAVTAGN YGGNLGRHHY
310
HLRDLLEKPA A
Length:311
Mass (Da):32,374
Last modified:January 23, 2007 - v4
Checksum:iF25FBE3918729428
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032114 Genomic DNA. Translation: AAC26972.1.
CP001510 Genomic DNA. Translation: ACS39600.1.
PIRiT45153.
RefSeqiYP_002962877.1. NC_012808.1.

Genome annotation databases

EnsemblBacteriaiACS39600; ACS39600; MexAM1_META1p1756.
GeneIDi7991719.
KEGGimea:Mex_1p1756.
PATRICi22509342. VBIMetExt101010_1746.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032114 Genomic DNA. Translation: AAC26972.1.
CP001510 Genomic DNA. Translation: ACS39600.1.
PIRiT45153.
RefSeqiYP_002962877.1. NC_012808.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS39600; ACS39600; MexAM1_META1p1756.
GeneIDi7991719.
KEGGimea:Mex_1p1756.
PATRICi22509342. VBIMetExt101010_1746.

Phylogenomic databases

eggNOGiCOG2037.
HOGENOMiHOG000230959.
KOiK00672.
OMAiCPAEAGI.
OrthoDBiEOG664CDN.

Enzyme and pathway databases

UniPathwayiUPA00562; UER00704.
BioCyciMetaCyc:MONOMER-4065.
MEXT272630:GBY6-1680-MONOMER.

Family and domain databases

Gene3Di3.30.70.520. 2 hits.
HAMAPiMF_00579. FTR.
InterProiIPR014053. ForMFR_H4MPT_ForTrfase.
IPR002770. ForMFR_H4MPT_ForTrfase_C.
IPR023447. ForMFR_H4MPT_ForTrfase_fd-like.
IPR022667. ForMFR_H4MPT_ForTrfase_N.
[Graphical view]
PfamiPF01913. FTR. 1 hit.
PF02741. FTR_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006414. Ftr_formyl_trnsf. 1 hit.
SUPFAMiSSF55112. SSF55112. 2 hits.
TIGRFAMsiTIGR03119. one_C_fhcD. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "C1 transfer enzymes and coenzymes linking methylotrophic bacteria and methanogenic Archaea."
    Chistoserdova L.V., Vorholt J.A., Thauer R.K., Lidstrom M.E.
    Science 281:99-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14718 / DSM 1338 / AM1.
  3. "Characterization of the formyltransferase from Methylobacterium extorquens AM1."
    Pomper B.K., Vorholt J.A.
    Eur. J. Biochem. 268:4769-4775(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-16, FUNCTION AS A FORMYLTRANSFERASE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
  4. "Generation of formate by the formyltransferase/hydrolase complex (Fhc) from Methylobacterium extorquens AM1."
    Pomper B.K., Saurel O., Milon A., Vorholt J.A.
    FEBS Lett. 523:133-137(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.

Entry informationi

Entry nameiFHCD_METEA
AccessioniPrimary (citable) accession number: Q49118
Secondary accession number(s): C5B136
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 100 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.