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Q49118

- FHCD_METEA

UniProt

Q49118 - FHCD_METEA

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Protein

Formyltransferase/hydrolase complex subunit D

Gene

fhcD

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the transformation of 5-formyl tetrahydromethanopterin (5-formyl-H4MPT) to methanofuran (MFR) and formate via the intermediate formylmethanofuran (formyl-MFR). Catalyzes the transfer of a formyl group from 5-formyl-H4MPT to MFR so as to produce tetrahydromethanopterin (H4MPT) and formyl-MFR, which is then hydrolyzed to formate and MFR.2 Publications

Catalytic activityi

Formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin.2 Publications

Kineticsi

  1. KM=30 µM for H4MPT1 Publication
  2. KM=50 µM for formyl-MFR1 Publication

Vmax=80 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 7.1 Publication

Pathwayi

GO - Molecular functioni

  1. formylmethanofuran-tetrahydromethanopterin N-formyltransferase activity Source: UniProtKB

GO - Biological processi

  1. formaldehyde catabolic process Source: UniProtKB-UniPathway
  2. one-carbon metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

One-carbon metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-4065.
MEXT272630:GBY6-1680-MONOMER.
UniPathwayiUPA00562; UER00704.

Names & Taxonomyi

Protein namesi
Recommended name:
Formyltransferase/hydrolase complex subunit D (EC:2.3.1.101)
Short name:
Ftr complex
Alternative name(s):
Formylmethanofuran--tetrahydromethanopterin formyltransferase
H4MPT formyltransferase
Gene namesi
Name:fhcD
Synonyms:ffsA
Ordered Locus Names:MexAM1_META1p1756
OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Taxonomic identifieri272630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000009081: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 311310Formyltransferase/hydrolase complex subunit DPRO_0000138124Add
BLAST

Interactioni

Subunit structurei

Octaheteromer. Part of the formyltransferase/hydrolase complex fhc; composed of FhcA, FhcB, FhcC and FhcD.2 Publications

Family & Domainsi

Sequence similaritiesi

Belongs to the FTR family.Curated

Phylogenomic databases

eggNOGiCOG2037.
HOGENOMiHOG000230959.
KOiK00672.
OMAiLERIGQC.
OrthoDBiEOG664CDN.

Family and domain databases

Gene3Di3.30.70.520. 2 hits.
HAMAPiMF_00579. FTR.
InterProiIPR014053. ForMFR_H4MPT_ForTrfase.
IPR002770. ForMFR_H4MPT_ForTrfase_C.
IPR023447. ForMFR_H4MPT_ForTrfase_fd-like.
IPR022667. ForMFR_H4MPT_ForTrfase_N.
[Graphical view]
PfamiPF01913. FTR. 1 hit.
PF02741. FTR_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006414. Ftr_formyl_trnsf. 1 hit.
SUPFAMiSSF55112. SSF55112. 2 hits.
TIGRFAMsiTIGR03119. one_C_fhcD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q49118-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDFTLNGIK VEDTFAEAFD VAGTAIIVTN DTPKWAMIAA TVMTGFATSV
60 70 80 90 100
IGCGAEAGID AELSPDETPD GRPGVRILLF GFEPNGLKDQ LLKRVGQCIL
110 120 130 140 150
TCPGTACFAG VEGPTKIKLG GAIRYFGDGF AVAKRLPDHE GKMRRYWRIP
160 170 180 190 200
VMDGEFLCED SVRAVDGAVG GGNLLFLGRK HADTLIVAEI AVEAAKAIPG
210 220 230 240 250
AILPFPGGIV RSGSKVGGRT KGMMASTNDA YCPTLKGRAG SALPPECGVV
260 270 280 290 300
LEIVIDALTS AAVAESMRAA LHAATEIGAQ HGLVAVTAGN YGGNLGRHHY
310
HLRDLLEKPA A
Length:311
Mass (Da):32,374
Last modified:January 23, 2007 - v4
Checksum:iF25FBE3918729428
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032114 Genomic DNA. Translation: AAC26972.1.
CP001510 Genomic DNA. Translation: ACS39600.1.
PIRiT45153.
RefSeqiYP_002962877.1. NC_012808.1.

Genome annotation databases

EnsemblBacteriaiACS39600; ACS39600; MexAM1_META1p1756.
GeneIDi7991719.
KEGGimea:Mex_1p1756.
PATRICi22509342. VBIMetExt101010_1746.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032114 Genomic DNA. Translation: AAC26972.1 .
CP001510 Genomic DNA. Translation: ACS39600.1 .
PIRi T45153.
RefSeqi YP_002962877.1. NC_012808.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACS39600 ; ACS39600 ; MexAM1_META1p1756 .
GeneIDi 7991719.
KEGGi mea:Mex_1p1756.
PATRICi 22509342. VBIMetExt101010_1746.

Phylogenomic databases

eggNOGi COG2037.
HOGENOMi HOG000230959.
KOi K00672.
OMAi LERIGQC.
OrthoDBi EOG664CDN.

Enzyme and pathway databases

UniPathwayi UPA00562 ; UER00704 .
BioCyci MetaCyc:MONOMER-4065.
MEXT272630:GBY6-1680-MONOMER.

Family and domain databases

Gene3Di 3.30.70.520. 2 hits.
HAMAPi MF_00579. FTR.
InterProi IPR014053. ForMFR_H4MPT_ForTrfase.
IPR002770. ForMFR_H4MPT_ForTrfase_C.
IPR023447. ForMFR_H4MPT_ForTrfase_fd-like.
IPR022667. ForMFR_H4MPT_ForTrfase_N.
[Graphical view ]
Pfami PF01913. FTR. 1 hit.
PF02741. FTR_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF006414. Ftr_formyl_trnsf. 1 hit.
SUPFAMi SSF55112. SSF55112. 2 hits.
TIGRFAMsi TIGR03119. one_C_fhcD. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "C1 transfer enzymes and coenzymes linking methylotrophic bacteria and methanogenic Archaea."
    Chistoserdova L.V., Vorholt J.A., Thauer R.K., Lidstrom M.E.
    Science 281:99-102(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14718 / DSM 1338 / AM1.
  3. "Characterization of the formyltransferase from Methylobacterium extorquens AM1."
    Pomper B.K., Vorholt J.A.
    Eur. J. Biochem. 268:4769-4775(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-16, FUNCTION AS A FORMYLTRANSFERASE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
  4. "Generation of formate by the formyltransferase/hydrolase complex (Fhc) from Methylobacterium extorquens AM1."
    Pomper B.K., Saurel O., Milon A., Vorholt J.A.
    FEBS Lett. 523:133-137(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.

Entry informationi

Entry nameiFHCD_METEA
AccessioniPrimary (citable) accession number: Q49118
Secondary accession number(s): C5B136
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 99 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3