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Protein

Formyltransferase/hydrolase complex subunit D

Gene

fhcD

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the transformation of 5-formyl tetrahydromethanopterin (5-formyl-H4MPT) to methanofuran (MFR) and formate via the intermediate formylmethanofuran (formyl-MFR). Catalyzes the transfer of a formyl group from 5-formyl-H4MPT to MFR so as to produce tetrahydromethanopterin (H4MPT) and formyl-MFR, which is then hydrolyzed to formate and MFR.2 Publications

Catalytic activityi

Formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin.2 Publications

Kineticsi

  1. KM=30 µM for H4MPT1 Publication
  2. KM=50 µM for formyl-MFR1 Publication
  1. Vmax=80 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 7.1 Publication

Pathwayi: formaldehyde degradation

This protein is involved in step 4 of the subpathway that synthesizes formate from formaldehyde (H(4)MPT route).
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. 5,6,7,8-tetrahydromethanopterin hydro-lyase (fae)
  2. Bifunctional protein MdtA (mtdA), NAD(P)-dependent methylenetetrahydromethanopterin dehydrogenase (mtdB)
  3. Methenyltetrahydromethanopterin cyclohydrolase (mch)
  4. Formyltransferase/hydrolase complex Fhc subunit B (fhcB), Formyltransferase/hydrolase complex Fhc subunit A (fhcA), Formyltransferase/hydrolase complex subunit D (fhcD), Formyltransferase/hydrolase complex Fhc subunit C (fhcC)
  5. no protein annotated in this organism
This subpathway is part of the pathway formaldehyde degradation, which is itself part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes formate from formaldehyde (H(4)MPT route), the pathway formaldehyde degradation and in One-carbon metabolism.

GO - Molecular functioni

GO - Biological processi

  • formaldehyde catabolic process Source: UniProtKB-UniPathway
  • one-carbon metabolic process Source: UniProtKB

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processOne-carbon metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-4065
UniPathwayiUPA00562; UER00704

Names & Taxonomyi

Protein namesi
Recommended name:
Formyltransferase/hydrolase complex subunit D (EC:2.3.1.101)
Short name:
Ftr complex
Alternative name(s):
Formylmethanofuran--tetrahydromethanopterin formyltransferase
H4MPT formyltransferase
Gene namesi
Name:fhcD
Synonyms:ffsA
Ordered Locus Names:MexAM1_META1p1756
OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Taxonomic identifieri272630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000009081 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001381242 – 311Formyltransferase/hydrolase complex subunit DAdd BLAST310

Interactioni

Subunit structurei

Octaheteromer. Part of the formyltransferase/hydrolase complex fhc; composed of FhcA, FhcB, FhcC and FhcD.2 Publications

Protein-protein interaction databases

STRINGi272630.MexAM1_META1p1756

Structurei

3D structure databases

SMRiQ49118
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FTR family.Curated

Phylogenomic databases

eggNOGiENOG4105FIE Bacteria
COG2037 LUCA
HOGENOMiHOG000230959
KOiK00672
OMAiCPAEAGI

Family and domain databases

Gene3Di3.30.70.520, 3 hits
HAMAPiMF_00579 FTR, 1 hit
InterProiView protein in InterPro
IPR014053 ForMFR_H4MPT_ForTrfase
IPR002770 ForMFR_H4MPT_ForTrfase_C
IPR023447 ForMFR_H4MPT_ForTrfase_fd-like
IPR022667 ForMFR_H4MPT_ForTrfase_N
PfamiView protein in Pfam
PF01913 FTR, 1 hit
PF02741 FTR_C, 1 hit
PIRSFiPIRSF006414 Ftr_formyl_trnsf, 1 hit
SUPFAMiSSF55112 SSF55112, 2 hits
TIGRFAMsiTIGR03119 one_C_fhcD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q49118-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDFTLNGIK VEDTFAEAFD VAGTAIIVTN DTPKWAMIAA TVMTGFATSV
60 70 80 90 100
IGCGAEAGID AELSPDETPD GRPGVRILLF GFEPNGLKDQ LLKRVGQCIL
110 120 130 140 150
TCPGTACFAG VEGPTKIKLG GAIRYFGDGF AVAKRLPDHE GKMRRYWRIP
160 170 180 190 200
VMDGEFLCED SVRAVDGAVG GGNLLFLGRK HADTLIVAEI AVEAAKAIPG
210 220 230 240 250
AILPFPGGIV RSGSKVGGRT KGMMASTNDA YCPTLKGRAG SALPPECGVV
260 270 280 290 300
LEIVIDALTS AAVAESMRAA LHAATEIGAQ HGLVAVTAGN YGGNLGRHHY
310
HLRDLLEKPA A
Length:311
Mass (Da):32,374
Last modified:January 23, 2007 - v4
Checksum:iF25FBE3918729428
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032114 Genomic DNA Translation: AAC26972.1
CP001510 Genomic DNA Translation: ACS39600.1
PIRiT45153
RefSeqiWP_003597588.1, NC_012808.1

Genome annotation databases

EnsemblBacteriaiACS39600; ACS39600; MexAM1_META1p1756
KEGGimea:Mex_1p1756

Similar proteinsi

Entry informationi

Entry nameiFHCD_METEA
AccessioniPrimary (citable) accession number: Q49118
Secondary accession number(s): C5B136
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 112 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health