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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Streptococcus pyogenes serotype M28 (strain MGAS6180)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei613 – 6131ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPYO319701:GHAD-192-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:M28_Spy0145
OrganismiStreptococcus pyogenes serotype M28 (strain MGAS6180)
Taxonomic identifieri319701 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000009292: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 833833Leucine--tRNA ligasePRO_1000009449Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi319701.M28_Spy0145.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5212"HIGH" regionAdd
BLAST
Motifi610 – 6145"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q48VJ7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTFYDHTAIE PKWQAFWADN HTFKTGTDAS KPKFYALDMF PYPSGAGLHV
60 70 80 90 100
GHPEGYTATD ILSRFKRAQG HNVLHPMGWD AFGLPAEQYA MDTGNDPAEF
110 120 130 140 150
TAENIANFKR QINALGFSYD WDREVNTTDP NYYKWTQWIF TKLYEKGLAY
160 170 180 190 200
EAEVPVNWVE ELGTAIANEE VLPDGTSERG GYPVVRKPMR QWMLKITAYA
210 220 230 240 250
ERLLEDLEEV DWPESIKDMQ RNWIGKSTGA NVTFKVKDTD KDFTVFTTRP
260 270 280 290 300
DTLFGATYAV LAPEHALVDA ITTADQAEAV ADYKRQASLK SDLARTDLAK
310 320 330 340 350
EKTGVWTGSY AINPVNGNEM PVWIADYVLA SYGTGAIMAV PAHDERDWEF
360 370 380 390 400
AKQFKLDIIP VLEGGNVEEA AFTEDGLHIN SDFLDGLDKA SAIAKMVEWL
410 420 430 440 450
EAEGVGNEKV TYRLRDWLFS RQRYWGEPIP IIHWEDGTST AVPESELPLV
460 470 480 490 500
LPVTKDIRPS GTGESPLANV TDWLEVTRED GVKGRRETNT MPQWAGSSWY
510 520 530 540 550
YLRYIDPHNT EKLADEELLK QWLPVDIYVG GAEHAVLHLL YARFWHKVLY
560 570 580 590 600
DLGVVPTKEP FQKLFNQGMI LGTSYRDSRG ALVATDKVEK RDGSFFHVET
610 620 630 640 650
GEELEQAPAK MSKSLKNVVN PDDVVEQYGA DTLRVYEMFM GPLDASIAWS
660 670 680 690 700
EEGLEGSRKF LDRVYRLITT KEITEENSGA LDKVYNETVK AVTEQVDQMK
710 720 730 740 750
FNTAIAQLMV FVNAANKEDK LFSDYAKGFV QLIAPFAPHL GEELWQVLTA
760 770 780 790 800
SGESISYVPW PSYDKSKLVE NDVEIVVQIK GKVKAKLVVA KDLSREELQE
810 820 830
VALANEKVQA EIAGKDIIKV IAVPNKLVNI VIK
Length:833
Mass (Da):93,890
Last modified:September 13, 2005 - v1
Checksum:i74FAB29FCDE3CA63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000056 Genomic DNA. Translation: AAX71259.1.
RefSeqiYP_279614.1. NC_007296.1.

Genome annotation databases

EnsemblBacteriaiAAX71259; AAX71259; M28_Spy0145.
GeneIDi3573147.
KEGGispb:M28_Spy0145.
PATRICi19743016. VBIStrPyo35950_0149.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000056 Genomic DNA. Translation: AAX71259.1.
RefSeqiYP_279614.1. NC_007296.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi319701.M28_Spy0145.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAX71259; AAX71259; M28_Spy0145.
GeneIDi3573147.
KEGGispb:M28_Spy0145.
PATRICi19743016. VBIStrPyo35950_0149.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciSPYO319701:GHAD-192-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of a serotype M28 strain of group A Streptococcus: potential new insights into puerperal sepsis and bacterial disease specificity."
    Green N.M., Zhang S., Porcella S.F., Nagiec M.J., Barbian K.D., Beres S.B., Lefebvre R.B., Musser J.M.
    J. Infect. Dis. 192:760-770(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MGAS6180.

Entry informationi

Entry nameiSYL_STRPM
AccessioniPrimary (citable) accession number: Q48VJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: January 7, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.