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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Streptococcus pyogenes serotype M28 (strain MGAS6180)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 18FADUniRule annotation6

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:M28_Spy0866
OrganismiStreptococcus pyogenes serotype M28 (strain MGAS6180)
Taxonomic identifieri319701 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000009292 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003464021 – 448Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOAdd BLAST448

Structurei

3D structure databases

ProteinModelPortaliQ48TI1.
SMRiQ48TI1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252054.
KOiK04094.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q48TI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSTATYIN VIGAGLAGSE AAYQIAKRGI PVKLYEMRGV KATPQHKTTN
60 70 80 90 100
FAELVCSNSF RGDSLTNAVG LLKEEMRRLD SIIMRNGEAN RVPAGGAMAV
110 120 130 140 150
DREGYAKSVT AELENHPLIE VIRDEITEIP DDAITVIATG PLTSDALAEK
160 170 180 190 200
IHALNGGDGF YFYDAAAPII DKSTIDMSKV YLKSRYDKGE AAYLNCPMTK
210 220 230 240 250
EEFMAFHDAL TNAEEAPLNA FEKEKYFEGC MPIEVMAKRG IKTMLYGPMK
260 270 280 290 300
PVGLEYPDDY TGPRDGEFKT PYAVVQLRQD NAAGSLYNIV GFQTHLKWGE
310 320 330 340 350
QKRVFQMIPG LENAEFVRYG VMHRNSYMDS PNLLTETFQS RNNPNLFFAG
360 370 380 390 400
QMTGVEGYVE SAASGLVAGT NAARLFKREE ALVFPQTTAI GSLPHYVTHA
410 420 430 440
DSKHFQPMNV NFGIIKELEG PRIRDKKERY EAIASRALAD LDTCLASL
Length:448
Mass (Da):49,481
Last modified:September 2, 2008 - v2
Checksum:i920CED2964BF80AC
GO

Sequence cautioni

The sequence AAX71979 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000056 Genomic DNA. Translation: AAX71979.1. Different initiation.
RefSeqiWP_021340800.1. NC_007296.1.

Genome annotation databases

EnsemblBacteriaiAAX71979; AAX71979; M28_Spy0866.
KEGGispb:M28_Spy0866.
PATRICi19744513. VBIStrPyo35950_0887.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000056 Genomic DNA. Translation: AAX71979.1. Different initiation.
RefSeqiWP_021340800.1. NC_007296.1.

3D structure databases

ProteinModelPortaliQ48TI1.
SMRiQ48TI1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAX71979; AAX71979; M28_Spy0866.
KEGGispb:M28_Spy0866.
PATRICi19744513. VBIStrPyo35950_0887.

Phylogenomic databases

HOGENOMiHOG000252054.
KOiK04094.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMFO_STRPM
AccessioniPrimary (citable) accession number: Q48TI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: November 2, 2016
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.