Q48SJ3 (ASNA_STRPM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	M28_Spy1207

Organism

Streptococcus pyogenes serotype M28 [Complete proteome] [HAMAP]

Taxonomic identifier

319700 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000017970

Sequences

	Sequence		Length	Mass (Da)
	Q48SJ3 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 255B344AB733920F	FASTA	330	37,399
	10 20 30 40 50 60 MKKSFIHQQE EISFVKNTFT QYLIAKLDVV EVQGPILSRV GDGMQDNLSG TENPVSVNVL 70 80 90 100 110 120 KIPNATFEVV HSLAKWKRHT LARFGFNEGE GLVVNMKALR PDEDSLDQTH SVYVDQWDWE 130 140 150 160 170 180 KVIPDGKRNL AYLKETVETI YKVIRLTELA VEARYDIEAV LPKKITFIHT EELVAKYPDL 190 200 210 220 230 240 TPKERENAIT KEFGAVFLIG IGGVLPDGKP HDGRAPDYDD WTTETENGYH GLNGDILVWN 250 260 270 280 290 300 DQLGSAFELS SMGIRVDEEA LKRQVEMTGD QDRLAFDWHK SLLNGLFPLT IGGGIGQSRM 310 320 330 VMFLLRKKHI GEVQTSVWPQ EVRDSYDNIL

References

[1]

"Genome sequence of a serotype M28 strain of group A Streptococcus: potential new insights into puerperal sepsis and bacterial disease specificity." Green N.M., Zhang S., Porcella S.F., Nagiec M.J., Barbian K.D., Beres S.B., Lefebvre R.B., Musser J.M. J. Infect. Dis. 192:760-770(2005) [PubMed: 16088825] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MGAS6180 / Serotype M28.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000056 Genomic DNA. Translation: AAX72317.1.
RefSeq	YP_280672.1. NC_007296.1.
3D structure databases
ProteinModelPortal	Q48SJ3.
SMR	Q48SJ3. Positions 4-330.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000032243; EBSTRP00000031075; EBSTRG00000032243.
GeneID	3573915.
GenomeReviews	Gene locus M28_Spy1207 in contig CP000056_GR.
KEGG	spb:M28_Spy1207.
PATRIC	19745225. VBIStrPyo35950_1243.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000027962.
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Enzyme and pathway databases
BioCyc	SPYO319701:M28_SPY1207-MONOMER.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_STRPM

Accession

Primary (citable) accession number: Q48SJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program