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Protein

Glutamate 5-kinase

Gene

proB

Organism
Streptococcus pyogenes serotype M28 (strain MGAS6180)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141ATPUniRule annotation
Binding sitei54 – 541SubstrateUniRule annotation
Binding sitei141 – 1411SubstrateUniRule annotation
Binding sitei157 – 1571Substrate; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi177 – 1782ATPUniRule annotation
Nucleotide bindingi219 – 2257ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate 5-kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPYO319701:GHAD-1479-MONOMER.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:M28_Spy1414
OrganismiStreptococcus pyogenes serotype M28 (strain MGAS6180)
Taxonomic identifieri319701 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000009292 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 272272Glutamate 5-kinasePRO_0000230069Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi319701.M28_Spy1414.

Structurei

3D structure databases

ProteinModelPortaliQ48RY6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246368.
KOiK00931.
OrthoDBiEOG6PGK7G.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q48RY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRQFEDVTR IVIKIGTSSL VLPTGKINLE KIDQLAFVIS SLMNKGKEVI
60 70 80 90 100
LVSSGAMGFG LDILKMEKRP TNLAKQQAVS SVGQVAMMSL YSQIFAHYQT
110 120 130 140 150
NVSQILLTRD VVVFPESLAN VTNAFESLIS LGIVPIVNEN DAVSVDEMDH
160 170 180 190 200
ATKFGDNDRL SAVVAGITKA DLLIMLSDID GLFDKNPTIY EDAQLRSHVA
210 220 230 240 250
VITQEIIASA GGAGSKFGTG GMLSKIQSAQ MVFENKGQMV LMNGANPRDI
260 270
LRVLEGQPLG TWFKQIEEVT HD
Length:272
Mass (Da):29,543
Last modified:April 4, 2006 - v2
Checksum:i371625F1BC65AAF2
GO

Sequence cautioni

The sequence AAX72524.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000056 Genomic DNA. Translation: AAX72524.1. Different initiation.
RefSeqiYP_280879.1. NC_007296.1.

Genome annotation databases

EnsemblBacteriaiAAX72524; AAX72524; M28_Spy1414.
KEGGispb:M28_Spy1414.
PATRICi19745663. VBIStrPyo35950_1450.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000056 Genomic DNA. Translation: AAX72524.1. Different initiation.
RefSeqiYP_280879.1. NC_007296.1.

3D structure databases

ProteinModelPortaliQ48RY6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi319701.M28_Spy1414.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAX72524; AAX72524; M28_Spy1414.
KEGGispb:M28_Spy1414.
PATRICi19745663. VBIStrPyo35950_1450.

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246368.
KOiK00931.
OrthoDBiEOG6PGK7G.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
BioCyciSPYO319701:GHAD-1479-MONOMER.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of a serotype M28 strain of group A Streptococcus: potential new insights into puerperal sepsis and bacterial disease specificity."
    Green N.M., Zhang S., Porcella S.F., Nagiec M.J., Barbian K.D., Beres S.B., Lefebvre R.B., Musser J.M.
    J. Infect. Dis. 192:760-770(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MGAS6180.

Entry informationi

Entry nameiPROB_STRPM
AccessioniPrimary (citable) accession number: Q48RY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: April 4, 2006
Last modified: April 29, 2015
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.