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Protein

Glutamate 5-kinase

Gene

proB

Organism
Streptococcus pyogenes serotype M28 (strain MGAS6180)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14ATPUniRule annotation1
Binding sitei54SubstrateUniRule annotation1
Binding sitei141SubstrateUniRule annotation1
Binding sitei157Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi177 – 178ATPUniRule annotation2
Nucleotide bindingi219 – 225ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:M28_Spy1414
OrganismiStreptococcus pyogenes serotype M28 (strain MGAS6180)
Taxonomic identifieri319701 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000009292 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002300691 – 272Glutamate 5-kinaseAdd BLAST272

Proteomic databases

PRIDEiQ48RY6

Structurei

3D structure databases

ProteinModelPortaliQ48RY6
SMRiQ48RY6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246368
KOiK00931

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q48RY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRQFEDVTR IVIKIGTSSL VLPTGKINLE KIDQLAFVIS SLMNKGKEVI
60 70 80 90 100
LVSSGAMGFG LDILKMEKRP TNLAKQQAVS SVGQVAMMSL YSQIFAHYQT
110 120 130 140 150
NVSQILLTRD VVVFPESLAN VTNAFESLIS LGIVPIVNEN DAVSVDEMDH
160 170 180 190 200
ATKFGDNDRL SAVVAGITKA DLLIMLSDID GLFDKNPTIY EDAQLRSHVA
210 220 230 240 250
VITQEIIASA GGAGSKFGTG GMLSKIQSAQ MVFENKGQMV LMNGANPRDI
260 270
LRVLEGQPLG TWFKQIEEVT HD
Length:272
Mass (Da):29,543
Last modified:April 4, 2006 - v2
Checksum:i371625F1BC65AAF2
GO

Sequence cautioni

The sequence AAX72524 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000056 Genomic DNA Translation: AAX72524.1 Different initiation.

Genome annotation databases

EnsemblBacteriaiAAX72524; AAX72524; M28_Spy1414
KEGGispb:M28_Spy1414

Similar proteinsi

Entry informationi

Entry nameiPROB_STRPM
AccessioniPrimary (citable) accession number: Q48RY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: April 4, 2006
Last modified: May 23, 2018
This is version 75 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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