Q48QH6 (HEM6_PSE14) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coproporphyrinogen-III oxidase, aerobic
Short name=Coprogen oxidase
Short name=Coproporphyrinogenase
EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	PSPPH_0026

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae pv. savastanoi

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333		PRO_1000019482
Regions
	Region	49 – 58	10	Important for dimerization By similarity
	Region	109 – 111	3	Substrate binding By similarity
	Region	241 – 276	36	Important for dimerization By similarity
	Region	259 – 264	6	Substrate binding By similarity
Sites
	Active site	107	1	Proton donor By similarity
	Binding site	93	1	Substrate By similarity
	Site	176	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48QH6 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 0AB30999D5C68125	FASTA	304	34,528
	10 20 30 40 50 60 MSTRTEAVKA YLLDLQDRIC TALEQEDGNA HFVEDAWTRP AGGGGRTRVI ENGAVIEKGG 70 80 90 100 110 120 VNFSHVFGSN LPPSASAHRP ELAGRGFEAL GVSLVIHPHN PHVPTSHANV RFFIAEKEGE 130 140 150 160 170 180 EPVWWFGGGF DLTPYYGVEE DCVHWHRVAE RACAPFGDDV YPRYKAWCDS YFHLKHRDEP 190 200 210 220 230 240 RGIGGLFFDD VNQWDFDTSF AFIRAIGDAF INAYLPIVRR RKAAAYTAQQ REFQEFRRGR 250 260 270 280 290 300 YVEFNLVYDR GTLFGLQSGG RTESILMSLP PQVRWGYDWK AAPGSEEARL TEYFLTDRDW LADN

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1448A / Race 6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000058 Genomic DNA. Translation: AAZ34851.1.
RefSeq	YP_272340.1. NC_005773.3.
3D structure databases
ProteinModelPortal	Q48QH6.
SMR	Q48QH6. Positions 1-301.
ModBase	Search...
Protein-protein interaction databases
STRING	Q48QH6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3557413.
GenomeReviews	Gene locus PSPPH_0026 in contig CP000058_GR.
KEGG	psp:PSPPH_0026.
NMPDR	fig|264730.3.peg.224.
PATRIC	19969011. VBIPseSyr78478_0030.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0408.
HOGENOM	HBG631180.
OMA	VKAYLLD.
PhylomeDB	Q48QH6.
ProtClustDB	PRK05330.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidase_aer. IPR018375. Coprogen_oxidase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
KO	K00228.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
SUPFAM	SSF102886. Coprogen_oxidas. 1 hit.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_PSE14

Accession

Primary (citable) accession number: Q48QH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program