Reviewed, UniProtKB/Swiss-Prot Q48PZ1 (DADA_PSE14)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase small subunit
    EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	PSPPH_0223

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity.
Catalytic activity	A D-amino acid + H(2)O + acceptor = a 2-oxo acid + NH(3) + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000066104
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q48PZ1-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: A229B02752C6F5C1	FASTA	433	47,154
	10 20 30 40 50 60 MRVLVLGSGV IGTTSAYYLA RAGFQVTVVD RQPAAAMETS FANAGQVSPG YASPWAAPGV 70 80 90 100 110 120 PLKAIKWLLQ RHAPLAIKAT ADIDQYLWMA QMLRNCTASR YTVNKERMVR LSEYSRDCLD 130 140 150 160 170 180 ELRLETGIAY EGRSLGTTQL FRTQAQLDNA AKDIAVLEQS GVPYELLDRD GIARVEPALA 190 200 210 220 230 240 GVTGILSGAL RLPNDQTGDC QLFTTRLAEM AVELGVEFRY GQNIERLDHA GDRINGVWID 250 260 270 280 290 300 GKLETADRYV LALGSYSPQL LKPLGIKAPV YPLKGYSLTV PITNPAMAPT STILDETYKV 310 320 330 340 350 360 AITRFDNRIR VGGMAEIAGF DLSLNPRRRE TLEMIVGDLY PQGGDLTQAS FWTGLRPTTP 370 380 390 400 410 420 DGTPIVGATP FRNLFLNTGH GTLGWTMACG SGRLLADLIA RKTPRISAEG LDISRYGNTQ 430 ENAQHVNPAP AHQ

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000058 Genomic DNA. Translation: AAZ34759.1.
RefSeq	YP_272528.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3557319.
GenomeReviews	Gene locus PSPPH_0223 in contig CP000058_GR.
KEGG	psp:PSPPH_0223.
NMPDR	fig|264730.3.peg.712.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q48PZ1.
Family and domain databases
HAMAP	MF_01202. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

DADA_PSE14

Accession

Primary (citable) accession number: Q48PZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 13, 2005
Last modified:	November 4, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)