Reviewed, UniProtKB/Swiss-Prot Q48PX1 (GSH1_PSE14)

Names and origin

Protein names

Recommended name:
    Glutamate--cysteine ligase
    EC=6.3.2.2
Alternative name(s):
    Gamma-glutamylcysteine synthetase
    Gamma-ECS
      Short name=GCS

Gene names

Name:	gshA
Ordered Locus Names:	PSPPH_0243

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP MF_00578
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP MF_00578
Sequence similarities	Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 535	535	Glutamate--cysteine ligase HAMAP MF_00578		PRO_1000025176

Sequences

	Sequence		Length	Mass (Da)
	Q48PX1-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 191AAA74067CC62A	FASTA	535	60,081
	10 20 30 40 50 60 MKDYTLSELL NRRLALLGER NNLSLLEQCL HGIERECLRV TATAELAQTP HPQALGAALT 70 80 90 100 110 120 NGQVTTDYSE SLLEFITPAL KNPAETIDSL DKIHRFAYSK LGDELLWSPS MPCPLPDEEH 130 140 150 160 170 180 TPIAYYGTSN IGKLKYVYRK GLALRYGKTM QCIAGIHYNF SLPEDAWALL KQTEDFAGDA 190 200 210 220 230 240 RDYQSHSYIA LIRNFRRYSW LLMYLFGASP ALDAGFLRGR KHQLEQHFDA DTLYLPYATS 250 260 270 280 290 300 LRMSDLGYQS DAQADLTPCY NDLVSYTDSL RKAVATPYKP YVDVGTHDQN GEWVQLNTNV 310 320 330 340 350 360 LQIENEYYSN IRPKRATYSG ERPIQALVAR GVQYVEVRCL DINPFLPTGI SLEQSRFIDA 370 380 390 400 410 420 FVLYCALEES QQLASHECSN ASSNFLAVVK EGRRPGLSLQ RNNSPVDLKT WATELLEKIT 430 440 450 460 470 480 PIARLLDQAQ GIDEHIKSIA VQQAKIDDAS LTPSAQVLAS MKAHNEGFTA FSLRQSQAHA 490 500 510 520 530 EYFRTHPLSA QEQADFEAQA KTSIEEQAEL EATEEVVDFD TFVGSYQASI LSISN

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000058 Genomic DNA. Translation: AAZ34309.1.
RefSeq	YP_272548.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3556857.
GenomeReviews	Gene locus PSPPH_0243 in contig CP000058_GR.
KEGG	psp:PSPPH_0243.
NMPDR	fig|264730.3.peg.732.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q48PX1.
OMA	Q48PX1. DTLYLPY.
Family and domain databases
HAMAP	MF_00578. [Tree]
InterPro	IPR007370. Glu_cys_ligase. IPR006334. Glut_cys_ligase. [Graphical view]
Pfam	PF04262. Glu_cys_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01434. glu_cys_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSH1_PSE14

Accession

Primary (citable) accession number: Q48PX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)