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Q48P88

- Q48P88_PSE14

UniProt

Q48P88 - Q48P88_PSE14

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Protein

Glutathione synthetase

Gene

gshB

Organism
Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Note: Binds 1 magnesium or manganese ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi282 – 2821Magnesium or manganeseUniRule annotation
Metal bindingi284 – 2841Magnesium or manganeseUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi152 – 20857ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutathione synthase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Glutathione biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciPSAV264730:GKDE-479-MONOMER.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshBUniRule annotationImported
Ordered Locus Names:PSPPH_0478Imported
OrganismiPseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)Imported
Taxonomic identifieri264730 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000551: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

Interactioni

Protein-protein interaction databases

STRINGi264730.PSPPH_0478.

Structurei

3D structure databases

ProteinModelPortaliQ48P88.
SMRiQ48P88. Positions 3-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 311187ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic GSH synthase family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000265022.
KOiK01920.
OMAiXRRTSAS.
OrthoDBiEOG6WMHWB.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q48P88-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVRLGIVMD PIERISYKKD SSLAMLLAAQ ERGWSLFYME QKDLYQDAGQ
60 70 80 90 100
ARARMKPLKV FADPAKWFEF EAEVDAGLDD LDVILMRKDL PFDMEFVYAT
110 120 130 140 150
YLLEQAERAG VLVVNKPQSL RDCNEKLFAT LFPQCTPPTL VSRRADIIRE
160 170 180 190 200
FAEKHGDVIL KPLDGMGGAS IFRHRAGDPN LSVILETLTA HGTQQIMAQG
210 220 230 240 250
YLPAIKDGDK RILMVDGEPV PYCLARIPAA GETRGNLAAG GRGEARPLSD
260 270 280 290 300
KDRWIAEQIG PTLREKGLLF VGLDVIGEHL TEINVTSPGG IREIDNAFGT
310
NIGGLLIDAI DKKLQARRG
Length:319
Mass (Da):35,280
Last modified:September 13, 2005 - v1
Checksum:i8C35B117D4BFAF96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000058 Genomic DNA. Translation: AAZ34205.1.
RefSeqiWP_011167499.1. NC_005773.3.
YP_272781.1. NC_005773.3.

Genome annotation databases

EnsemblBacteriaiAAZ34205; AAZ34205; PSPPH_0478.
GeneIDi3556749.
KEGGipsp:PSPPH_0478.
PATRICi19969988. VBIPseSyr78478_0519.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000058 Genomic DNA. Translation: AAZ34205.1 .
RefSeqi WP_011167499.1. NC_005773.3.
YP_272781.1. NC_005773.3.

3D structure databases

ProteinModelPortali Q48P88.
SMRi Q48P88. Positions 3-315.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264730.PSPPH_0478.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ34205 ; AAZ34205 ; PSPPH_0478 .
GeneIDi 3556749.
KEGGi psp:PSPPH_0478.
PATRICi 19969988. VBIPseSyr78478_0519.

Phylogenomic databases

eggNOGi COG0189.
HOGENOMi HOG000265022.
KOi K01920.
OMAi XRRTSAS.
OrthoDBi EOG6WMHWB.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00210 .
BioCyci PSAV264730:GKDE-479-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPi MF_00162. GSH_S.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
Pfami PF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view ]
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01380. glut_syn. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1448A / Race 6Imported.

Entry informationi

Entry nameiQ48P88_PSE14
AccessioniPrimary (citable) accession number: Q48P88
Entry historyi
Integrated into UniProtKB/TrEMBL: September 13, 2005
Last sequence update: September 13, 2005
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3