Reviewed, UniProtKB/Swiss-Prot Q48NS2 (GPH_PSE14)

Names and origin

Protein names

Recommended name:
    Phosphoglycolate phosphatase
      Short name=PGPase
      Short name=PGP
    EC=3.1.3.18

Gene names

Ordered Locus Names:

PSPPH_0648

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the dephosphorylation of 2-phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stress By similarity.
Catalytic activity	2-phosphoglycolate + H2O = glycolate + phosphate. HAMAP MF_00495
Pathway	Organic acid metabolism; glycolic acid biosynthesis; glycolic acid from 2-phosphoglycolic acid: step 1/1. HAMAP MF_00495
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. CbbY/cbbZ/gph/yieH family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	phosphoglycolate phosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Phosphoglycolate phosphatase HAMAP MF_00495		PRO_0000238169
Sites
	Active site	19	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48NS2-1 [UniParc]. Last modified May 30, 2006. Version 2. Checksum: FF9B6D48686571FF	FASTA	272	30,016
	10 20 30 40 50 60 MSGFEQLFAG KLPKLVMFDL DGTLVDSVPD LAVAVDTMLA ELGRPIAGLE SVRAWVGNGA 70 80 90 100 110 120 PVLVRRALAN HLDHSGVDDE LAEQGLEIFM RAYAQKHEFT VVYPGVRETL KWLQKMGVEM 130 140 150 160 170 180 ALITNKPERF VAPLLDEMKL GRFFRWIIGG DTMPQKKPDP AALFFVMKMA GVPASQALFV 190 200 210 220 230 240 GDSRSDVQAA KAAGVACVAL SYGYNHGRPI AEENPAMVID DLRKLIPGCL DMDAEILLPD 250 260 270 INSPSSRESI VVVTRKLWMK VIKALARWRW RA

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000058 Genomic DNA. Translation: AAZ36472.1. Different initiation.
RefSeq	YP_272947.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3559092.
GenomeReviews	Gene locus PSPPH_0648 in contig CP000058_GR.
KEGG	psp:PSPPH_0648.
NMPDR	fig|264730.3.peg.1085.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q48NS2.
Family and domain databases
HAMAP	MF_00495. [Tree]
InterPro	IPR005834. Dehalogen-like_hydro. IPR006439. HAD-SF_hydro_IA_v1. IPR006402. HAD-SF_hydro_IA_v3. IPR005833. Haloacid_DH/epoxide_hydro. IPR006346. PGP_bact. [Graphical view]
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00413. HADHALOGNASE.
TIGRFAMs	TIGR01549. HAD-SF-IA-v1. 1 hit. TIGR01509. HAD-SF-IA-v3. 1 hit. TIGR01449. PGP_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GPH_PSE14

Accession

Primary (citable) accession number: Q48NS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	May 30, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)