Q48NK6 (SYI_PSE14) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	PSPPH_0721

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae group › Pseudomonas syringae group genomosp. 2 › Pseudomonas syringae pv. savastanoi › Pseudomonas syringae pv. phaseolicola

Protein attributes

Sequence length	943 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 943	943	Isoleucine--tRNA ligase HAMAP-Rule MF_02002		PRO_0000098448
Regions
	Motif	58 – 68	11	"HIGH" region HAMAP-Rule MF_02002
	Motif	608 – 612	5	"KMSKS" region HAMAP-Rule MF_02002
Sites
	Metal binding	906	1	Zinc By similarity
	Metal binding	909	1	Zinc By similarity
	Metal binding	926	1	Zinc By similarity
	Metal binding	929	1	Zinc By similarity
	Binding site	567	1	Aminoacyl-adenylate By similarity
	Binding site	611	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48NK6 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 4D94770A8B07B8D9	FASTA	943	105,857
	10 20 30 40 50 60 MTDYKATLNL PDTAFPMKAG LPQREPQTLQ RWDSIGLYQK LREIGKDRPK FVLHDGPPYA 70 80 90 100 110 120 NGNIHIGHAV NKILKDMILR SKTLAGFDAP YVPGWDCHGL PIEHKVEVTH GKNLSADRTR 130 140 150 160 170 180 ELCRAYASEQ IEGQKSEFIR LGVLGDWSNP YLTMNFANEA GEIRALAEMV KGGFVFKGLK 190 200 210 220 230 240 PVNWCFDCGS ALAEAEVEYQ DKKSSTIDVA FLIADEAKLA AAFGLPALGK PASIVIWTTT 250 260 270 280 290 300 PWTIPANQAL NVHPEFEYAL VDVGDKLLVL AAELVESCLA RYKLEGTVIA TTTGQALELI 310 320 330 340 350 360 NFRHPFYDRL SPVYLADYVE LGAGTGIVHS SPAYGVDDFN ICKQYGLSND DIISPVQSNG 370 380 390 400 410 420 VYVESLEFFG GQFIFKANQN IIDKLVEVGS LMDTETISHS YMHCWRHKSP LIYRATAQWF 430 440 450 460 470 480 VGMDKQPESG ETLRKRAVKA IEDTEFVPAW GQARLHSMIA NRPDWCISRQ RNWGVPIPFF 490 500 510 520 530 540 LHKESGDLHP RTVELMEEVA QRVEKEGIEA WFKLDASELL GDEAAKYDKI SDTLDVWFDS 550 560 570 580 590 600 GTTHWHVLRG SHPMGHESGP RADLYLEGSD QHRGWFHSSL LTGCMLDDHA PYRELLTHGF 610 620 630 640 650 660 VVDENGRKMS KSLNNVVAPQ KVNDSLGADI MRLWVSATDY SGEMAVSDQI LQRSADAYRR 670 680 690 700 710 720 IRNTARFLLS NLSGFNPATD ILPAEEMLAL DRWAVDRTLL LQRELQEHYG EYRFWNVYSK 730 740 750 760 770 780 IHNFCVQELG GFYLDIIKDR QYTTAADSTA RRSCQTALFH ISEALVRWIT PILAFTADEL 790 800 810 820 830 840 WQFLPGERNE SVMLNTWYEG LTEMPADFEM DRAYWERIMA VKTSVNKEME NLRAAKAIGG 850 860 870 880 890 900 NLQAEVTLYA EDSLIADLSK LSNELRFVLI TSTASVAPLV SAPADAVVTE VAGLKLKVLK 910 920 930 940 SSHAKCARCW HHREDVGVNP EHPEICGRCV DNISGAGEVR HYA

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1448A / Race 6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000058 Genomic DNA. Translation: AAZ37532.1.
RefSeq	YP_273013.1. NC_005773.3.
3D structure databases
ProteinModelPortal	Q48NK6.
ModBase	Search...
Protein-protein interaction databases
STRING	264730.PSPPH_0721.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ37532; AAZ37532; PSPPH_0721.
GeneID	3560188.
KEGG	psp:PSPPH_0721.
PATRIC	19970509. VBIPseSyr78478_0772.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0060.
HOGENOM	HOG000246402.
KO	K01870.
OMA	KQVLTHG.
ProtClustDB	PRK05743.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
HAMAP	MF_02002. Ile_tRNA_synth_type1.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023585. Ile-tRNA-ligase_type1. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. IPR010663. Znf_DNA_glyclase/IsotRNA_synth. [Graphical view]
PANTHER	PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_PSE14

Accession

Primary (citable) accession number: Q48NK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program