Q48ND9 (CHEB1_PSE14) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase 1
EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	PSPPH_0800

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae pv. savastanoi

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000225473
Regions
	Domain	7 – 124	118	Response regulatory
	Domain	170 – 358	189	CheB-type methylesterase
Sites
	Active site	182	1	By similarity
	Active site	208	1	By similarity
	Active site	304	1	By similarity
Amino acid modifications
	Modified residue	58	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48ND9 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: D11A1C8A9EBFABC4	FASTA	358	37,875
	10 20 30 40 50 60 MPNKKISVLL VDDSAVVRQV LVAILNDTPD IHVMGAATDP IFAMAKLAQE WPDVIVLDVE 70 80 90 100 110 120 MPRMDGITFL KKIMSERPTP VVICSSLTQK GAETSLQALS AGAVEIITKP TTGLKNFLID 130 140 150 160 170 180 SAAELVAAVR AAANSNVKNL GKRMAPPVLA PASKLTADAI LPAASGHAMA QTTERIVAIG 190 200 210 220 230 240 TSTGGTQALE AVLTALPRVC PGMVIVQHMP EKFTASFAER LNGLSQIEVP EARNNDRILP 250 260 270 280 290 300 GLALIAPGGK HMMVTRSGAS YYVQVIDGPL VNRHRPSVDV LFRSVAKFAG KNATGIIMTG 310 320 330 340 350 MGDDGARGLK EMLDAGSSTV AQDEASCVVF GMPKEAIKLN AAQRIMPLQE IHQAILHR

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1448A / Race 6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000058 Genomic DNA. Translation: AAZ36844.1.
RefSeq	YP_273082.1. NC_005773.3.
3D structure databases
ProteinModelPortal	Q48ND9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q48ND9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3559474.
GenomeReviews	Gene locus PSPPH_0800 in contig CP000058_GR.
KEGG	psp:PSPPH_0800.
NMPDR	fig|264730.3.peg.632.
PATRIC	19970671. VBIPseSyr78478_0849.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	PGGKHML.
PhylomeDB	Q48ND9.
ProtClustDB	PRK00742.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_PSE14

Accession

Primary (citable) accession number: Q48ND9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program