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Protein

Alginate lyase

Gene

algL

Organism
Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6) (Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6))
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the depolymerization of alginate by cleaving the beta-1,4 glycosidic bond between two adjacent sugar residues via a beta-elimination mechanism. May serve to degrade mislocalized alginate that is trapped in the periplasmic space.UniRule annotation

Catalytic activityi

Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and beta-D-mannuronate at their reducing end.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei258SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported

Protein family/group databases

CAZyiPL5. Polysaccharide Lyase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Alginate lyaseUniRule annotation (EC:4.2.2.3UniRule annotation)
Alternative name(s):
Poly(beta-D-mannuronate) lyaseUniRule annotation
Gene namesi
Name:algLUniRule annotationImported
Ordered Locus Names:PSPPH_1111Imported
OrganismiPseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6) (Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6))Imported
Taxonomic identifieri264730 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000551 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

PeriplasmUniRule annotation

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28UniRule annotationAdd BLAST28
ChainiPRO_500507868529 – 380Alginate lyaseUniRule annotationAdd BLAST352

Interactioni

Protein-protein interaction databases

STRINGi264730.PSPPH_1111.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 305Alginate_lyaseInterPro annotationAdd BLAST241

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 68Substrate bindingUniRule annotation2
Regioni140 – 141Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the polysaccharide lyase 5 family.UniRule annotation

Keywords - Domaini

SignalUniRule annotation

Phylogenomic databases

eggNOGiENOG4105FN4. Bacteria.
ENOG4111N6J. LUCA.
HOGENOMiHOG000247392.
KOiK01729.
OMAiAAWSVMA.

Family and domain databases

CDDicd00244. AlgLyase. 1 hit.
Gene3Di1.50.10.110. 1 hit.
HAMAPiMF_00557. Alginate_lyase. 1 hit.
InterProiIPR022859. Alginate_lyase.
IPR008397. Alginate_lyase_dom.
IPR008929. Chondroitin_lyas.
[Graphical view]
PfamiPF05426. Alginate_lyase. 1 hit.
[Graphical view]
SUPFAMiSSF48230. SSF48230. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q48MJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTPKLIRPT LLSMAILSSM AWATGASAAL VPPKGYDAPI EKMKTGDHNF
60 70 80 90 100
SCEAIPKPYT DKLVFRSKYE GSDKARATLN AVSEEAFRDA TKDITDLERG
110 120 130 140 150
VSKVVMQYMR DGRPEQLDCA LNMMTTWAKA DALESREFNH TGKSMRKWAL
160 170 180 190 200
GSMSSAYLRL KFSESHPLAN RQQDAKIIEA WFSKLADQVV SDWSNLPLEK
210 220 230 240 250
INNHSYWAAW SVMATAVATN RQDLFDWAVK EYKVAANQVD KDGFLPNEMK
260 270 280 290 300
RRQRALSYHN YALPPLAMIA SFAQANGVDL RPENNGALKR LGDRVLAGVK
310 320 330 340 350
DPSIFAEHNG EKQDMTDLKK DPKFAWLEPY CSLYPCSPDV LEEKHEKQPF
360 370 380
KTFRLGGDLT KVYDPTHEKG DKGEGDNDGS
Length:380
Mass (Da):42,708
Last modified:September 13, 2005 - v1
Checksum:i64F0BCB207F742A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000058 Genomic DNA. Translation: AAZ36796.1.
RefSeqiWP_004658320.1. NC_005773.3.

Genome annotation databases

EnsemblBacteriaiAAZ36796; AAZ36796; PSPPH_1111.
KEGGipsp:PSPPH_1111.
PATRICi19971322. VBIPseSyr78478_1167.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000058 Genomic DNA. Translation: AAZ36796.1.
RefSeqiWP_004658320.1. NC_005773.3.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264730.PSPPH_1111.

Protein family/group databases

CAZyiPL5. Polysaccharide Lyase Family 5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ36796; AAZ36796; PSPPH_1111.
KEGGipsp:PSPPH_1111.
PATRICi19971322. VBIPseSyr78478_1167.

Phylogenomic databases

eggNOGiENOG4105FN4. Bacteria.
ENOG4111N6J. LUCA.
HOGENOMiHOG000247392.
KOiK01729.
OMAiAAWSVMA.

Family and domain databases

CDDicd00244. AlgLyase. 1 hit.
Gene3Di1.50.10.110. 1 hit.
HAMAPiMF_00557. Alginate_lyase. 1 hit.
InterProiIPR022859. Alginate_lyase.
IPR008397. Alginate_lyase_dom.
IPR008929. Chondroitin_lyas.
[Graphical view]
PfamiPF05426. Alginate_lyase. 1 hit.
[Graphical view]
SUPFAMiSSF48230. SSF48230. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQ48MJ3_PSE14
AccessioniPrimary (citable) accession number: Q48MJ3
Entry historyi
Integrated into UniProtKB/TrEMBL: September 13, 2005
Last sequence update: September 13, 2005
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.