Q48MF3 (RLMF_PSE14) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	PSPPH_1152

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae pv. savastanoi

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.
Sequence caution	The sequence AAZ34734.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349937

Sequences

	Sequence		Length	Mass (Da)
	Q48MF3 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: E3F67525ECAE4F96	FASTA	328	36,246
	10 20 30 40 50 60 MTDTRKPPRK KPQRPAKPAA PREKATLHPR NRHQGHYDFA KLIKSSPELA AFVILNPYGK 70 80 90 100 110 120 ESIDFADPQA VRVFNRALLK AFYGIAHWDI PADYLCPPIP GRADYLHFLA DLLAEDNEGV 130 140 150 160 170 180 IPRGASIKAL DIGTGANCIY PLLGHSDYGW QFVGSDIDST AIAAATTIIK ANGLSKAISV 190 200 210 220 230 240 RQQDNRKQIL LGLLDSSERF HVSLCNPPFH ASLDEAQRGS QRKWRALGKA DPKRKLPVLN 250 260 270 280 290 300 FGGQSQELWC EGGEIGFVTR LIQESATLPS QVVWFSTLVS KASNLPPIQN ALKKAGALEV 310 320 KVIEMGQGQK QSRFVAWTFL DKAQRTPH

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1448A / Race 6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000058 Genomic DNA. Translation: AAZ34734.1. Different initiation.
RefSeq	YP_273419.2. NC_005773.3.
3D structure databases
ProteinModelPortal	Q48MF3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q48MF3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3557294.
GenomeReviews	Gene locus PSPPH_1152 in contig CP000058_GR.
KEGG	psp:PSPPH_1152.
NMPDR	fig|264730.3.peg.470.
PATRIC	19971404. VBIPseSyr78478_1209.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3129.
HOGENOM	HBG747880.
ProtClustDB	PRK11727.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_PSE14

Accession

Primary (citable) accession number: Q48MF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program