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Q48LY5

- LEU1_PSE14

UniProt

Q48LY5 - LEU1_PSE14

Protein

2-isopropylmalate synthase

Gene

leuA

Organism
Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).UniRule annotation

    Catalytic activityi

    Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.UniRule annotation

    Pathwayi

    GO - Molecular functioni

    1. 2-isopropylmalate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. leucine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

    Enzyme and pathway databases

    BioCyciPSAV264730:GKDE-1332-MONOMER.
    UniPathwayiUPA00048; UER00070.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-isopropylmalate synthaseUniRule annotation (EC:2.3.3.13UniRule annotation)
    Alternative name(s):
    Alpha-IPM synthaseUniRule annotation
    Alpha-isopropylmalate synthaseUniRule annotation
    Gene namesi
    Name:leuAUniRule annotation
    Ordered Locus Names:PSPPH_1329
    OrganismiPseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)
    Taxonomic identifieri264730 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000000551: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5565562-isopropylmalate synthasePRO_1000025031Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi264730.PSPPH_1329.

    Structurei

    3D structure databases

    ProteinModelPortaliQ48LY5.
    SMRiQ48LY5. Positions 6-547.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0119.
    HOGENOMiHOG000110941.
    KOiK01649.
    OMAiVERCNQI.
    OrthoDBiEOG62G5PZ.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00572. LeuA_type2.
    InterProiIPR013709. 2-isopropylmalate_synth_dimer.
    IPR002034. AIPM/Hcit_synth_CS.
    IPR013785. Aldolase_TIM.
    IPR005668. IPM_Synthase.
    IPR000891. PYR_CT.
    [Graphical view]
    PfamiPF00682. HMGL-like. 1 hit.
    PF08502. LeuA_dimer. 1 hit.
    [Graphical view]
    SMARTiSM00917. LeuA_dimer. 1 hit.
    [Graphical view]
    SUPFAMiSSF110921. SSF110921. 1 hit.
    TIGRFAMsiTIGR00970. leuA_yeast. 1 hit.
    PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
    PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
    PS50991. PYR_CT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q48LY5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTMLKDPSKK YRAFPTIDLP DRTWPSKTID AAPIWCSSDL RDGNQSLIEP    50
    MDAAKKLRFW KTLVSVGVKE IEASFPSASQ TDFDFVRTLI EDGHIPDDTT 100
    IQVLTQARED LIARTFESLR GAKKAIVHLY NATSPSFRRI VFNQDKAGVK 150
    EIAVNAAKLF VKYAAQQPET QWTFEYSPET FSATELEFAK EVCDAVIEVW 200
    NPTPENKVIL NLPATVEVAT PNIYADQIEW FGRNITRRDS VLISLHTHND 250
    RGTGVAATEL GLMAGADRVE GCLFGNGERT GNVDLVTVAL NLYTQGIDPE 300
    LDFSDIDGVR KVVEECNQIP VHPRHPYVGD LVHTAFSGSH QDAIRKGFTQ 350
    QKDGELWEVP YLPIDPADIG RSYEAVIRVN SQSGKGGITY LLEQEYGISL 400
    PRRMQIEFSQ VVQGETDRLG LEMSAQQIHS LLRREYLQAN TPYALISHKL 450
    QEENGNSAVD AEVHVDGETQ HWRGKGKGAL EALVAGLPVA VEIMDYNEHA 500
    IGSGTTAKAA AYIELRVNGE RAVHGVGIDE NITTASFRAL FSALNRSLSQ 550
    TQAKAA 556
    Length:556
    Mass (Da):61,525
    Last modified:September 13, 2005 - v1
    Checksum:iD5EF436B9D587FB9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000058 Genomic DNA. Translation: AAZ36794.1.
    RefSeqiYP_273587.1. NC_005773.3.

    Genome annotation databases

    EnsemblBacteriaiAAZ36794; AAZ36794; PSPPH_1329.
    GeneIDi3559420.
    KEGGipsp:PSPPH_1329.
    PATRICi19971781. VBIPseSyr78478_1396.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000058 Genomic DNA. Translation: AAZ36794.1 .
    RefSeqi YP_273587.1. NC_005773.3.

    3D structure databases

    ProteinModelPortali Q48LY5.
    SMRi Q48LY5. Positions 6-547.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 264730.PSPPH_1329.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ36794 ; AAZ36794 ; PSPPH_1329 .
    GeneIDi 3559420.
    KEGGi psp:PSPPH_1329.
    PATRICi 19971781. VBIPseSyr78478_1396.

    Phylogenomic databases

    eggNOGi COG0119.
    HOGENOMi HOG000110941.
    KOi K01649.
    OMAi VERCNQI.
    OrthoDBi EOG62G5PZ.

    Enzyme and pathway databases

    UniPathwayi UPA00048 ; UER00070 .
    BioCyci PSAV264730:GKDE-1332-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00572. LeuA_type2.
    InterProi IPR013709. 2-isopropylmalate_synth_dimer.
    IPR002034. AIPM/Hcit_synth_CS.
    IPR013785. Aldolase_TIM.
    IPR005668. IPM_Synthase.
    IPR000891. PYR_CT.
    [Graphical view ]
    Pfami PF00682. HMGL-like. 1 hit.
    PF08502. LeuA_dimer. 1 hit.
    [Graphical view ]
    SMARTi SM00917. LeuA_dimer. 1 hit.
    [Graphical view ]
    SUPFAMi SSF110921. SSF110921. 1 hit.
    TIGRFAMsi TIGR00970. leuA_yeast. 1 hit.
    PROSITEi PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
    PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
    PS50991. PYR_CT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1448A / Race 6.

    Entry informationi

    Entry nameiLEU1_PSE14
    AccessioniPrimary (citable) accession number: Q48LY5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3