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Protein

2-isopropylmalate synthase

Gene

leuA

Organism
Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).UniRule annotation

Catalytic activityi

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 2-isopropylmalate synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

BioCyciPSAV264730:GKDE-1332-MONOMER.
UniPathwayiUPA00048; UER00070.

Names & Taxonomyi

Protein namesi
Recommended name:
2-isopropylmalate synthaseUniRule annotation (EC:2.3.3.13UniRule annotation)
Alternative name(s):
Alpha-IPM synthaseUniRule annotation
Alpha-isopropylmalate synthaseUniRule annotation
Gene namesi
Name:leuAUniRule annotation
Ordered Locus Names:PSPPH_1329
OrganismiPseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)
Taxonomic identifieri264730 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000551: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5565562-isopropylmalate synthasePRO_1000025031Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi264730.PSPPH_1329.

Structurei

3D structure databases

ProteinModelPortaliQ48LY5.
SMRiQ48LY5. Positions 6-547.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000110941.
KOiK01649.
OMAiVERCNQI.
OrthoDBiEOG62G5PZ.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00572. LeuA_type2.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005668. IPM_Synthase.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00970. leuA_yeast. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q48LY5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMLKDPSKK YRAFPTIDLP DRTWPSKTID AAPIWCSSDL RDGNQSLIEP
60 70 80 90 100
MDAAKKLRFW KTLVSVGVKE IEASFPSASQ TDFDFVRTLI EDGHIPDDTT
110 120 130 140 150
IQVLTQARED LIARTFESLR GAKKAIVHLY NATSPSFRRI VFNQDKAGVK
160 170 180 190 200
EIAVNAAKLF VKYAAQQPET QWTFEYSPET FSATELEFAK EVCDAVIEVW
210 220 230 240 250
NPTPENKVIL NLPATVEVAT PNIYADQIEW FGRNITRRDS VLISLHTHND
260 270 280 290 300
RGTGVAATEL GLMAGADRVE GCLFGNGERT GNVDLVTVAL NLYTQGIDPE
310 320 330 340 350
LDFSDIDGVR KVVEECNQIP VHPRHPYVGD LVHTAFSGSH QDAIRKGFTQ
360 370 380 390 400
QKDGELWEVP YLPIDPADIG RSYEAVIRVN SQSGKGGITY LLEQEYGISL
410 420 430 440 450
PRRMQIEFSQ VVQGETDRLG LEMSAQQIHS LLRREYLQAN TPYALISHKL
460 470 480 490 500
QEENGNSAVD AEVHVDGETQ HWRGKGKGAL EALVAGLPVA VEIMDYNEHA
510 520 530 540 550
IGSGTTAKAA AYIELRVNGE RAVHGVGIDE NITTASFRAL FSALNRSLSQ

TQAKAA
Length:556
Mass (Da):61,525
Last modified:September 13, 2005 - v1
Checksum:iD5EF436B9D587FB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000058 Genomic DNA. Translation: AAZ36794.1.
RefSeqiYP_273587.1. NC_005773.3.

Genome annotation databases

EnsemblBacteriaiAAZ36794; AAZ36794; PSPPH_1329.
GeneIDi3559420.
KEGGipsp:PSPPH_1329.
PATRICi19971781. VBIPseSyr78478_1396.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000058 Genomic DNA. Translation: AAZ36794.1.
RefSeqiYP_273587.1. NC_005773.3.

3D structure databases

ProteinModelPortaliQ48LY5.
SMRiQ48LY5. Positions 6-547.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264730.PSPPH_1329.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ36794; AAZ36794; PSPPH_1329.
GeneIDi3559420.
KEGGipsp:PSPPH_1329.
PATRICi19971781. VBIPseSyr78478_1396.

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000110941.
KOiK01649.
OMAiVERCNQI.
OrthoDBiEOG62G5PZ.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00070.
BioCyciPSAV264730:GKDE-1332-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00572. LeuA_type2.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005668. IPM_Synthase.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00970. leuA_yeast. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1448A / Race 6.

Entry informationi

Entry nameiLEU1_PSE14
AccessioniPrimary (citable) accession number: Q48LY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: February 4, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.