Reviewed, UniProtKB/Swiss-Prot Q48LX3 (PUR4_PSE14)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	PSPPH_1341

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae pv. savastanoi

Protein attributes

Sequence length	1298 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm HAMAP MF_00419.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1298	1298	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000264589
Regions
	Domain	1045 – 1298	254	Glutamine amidotransferase type-1
	Nucleotide binding	305 – 316	12	ATP Potential
Sites
	Active site	1138	1	Nucleophile By similarity
	Active site	1263	1	By similarity
	Active site	1265	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48LX3-1 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: 14EA86888AF47994	FASTA	1,298	140,902
	10 20 30 40 50 60 MLILRGAPAL SAFRHSKLLE QLKQKVSAVS GLYAEFAHFA DVNDVLTSEE QQVLDRLLKY 70 80 90 100 110 120 GPSVPVQEPS GRLFLVLPRF GTISPWSSKA SDIARNCGLT KIQRIERGIA FYVEGQFSEA 130 140 150 160 170 180 QAQAIADSLH DRMTQLVLGD HEQAASLFSH AQPKPLTAVD ILGGGRAALE KANVELGLAL 190 200 210 220 230 240 AEDEIDYLIT SFNGLGRNPH DIELMMFAQA NSEHCRHKIF NASWDIDGQS QEKSLFGMIK 250 260 270 280 290 300 NTYQMHSEGV LSAYKDNASV IVGNVAGRFF PDPETRQYGA VQEPVHILMK VETHNHPTAI 310 320 330 340 350 360 APFPGAATGS GGEIRDEGAT GRGAKPKAGL TGFTVSNLQI PGFVQPWEVP YGKPERIVTA 370 380 390 400 410 420 LDIMIEGPLG GAAFNNEFGR PALTGYFRTF EQSITTPHGD EVRGYHKPIM LAGGMGNIRE 430 440 450 460 470 480 DHVQKGEITV GSKLIVLGGP AMLIGLGGGA ASSMATGTSS ADLDFASVQR ENPEMERRCQ 490 500 510 520 530 540 EVIDRCWQLG DRNPISFIHD VGAGGLSNAF PELVNDGDRG GRFELRNVPN DEPGMAPLEI 550 560 570 580 590 600 WSNESQERYV LAVGVEDFDR FKAICERERC PFAVVGEATA EPQLTVTDSH FGNSPVDMPL 610 620 630 640 650 660 EVLLGKVPRM HRSVEREAEI GDDFDPSTLD IEESVQRVLR HPAVASKSFL ITIGDRSITG 670 680 690 700 710 720 LVARDQMVGP WQVPVADCAV TATSFDVNTG EAMAMGERTP LALLDAPASG RMAIGETLTN 730 740 750 760 770 780 IAASCIEKLS DIKLSANWMS AAGHPGEDAR LYDTVKAVGM ELCPELGITI PVGKDSMSMK 790 800 810 820 830 840 TRWSDEGTEK SVTSPLSLIV TGFAPVVDIR QTLTPELRMD KGITDLILID LGRGQNRMGA 850 860 870 880 890 900 SILAQTHGKL GRVAPDVDDA EDLKAFFAVI QGLNSDGHIL SYHDRSDGGL LVSTLEMAFA 910 920 930 940 950 960 GHCGLNLHLD GVADNVSELS AILFNEELGA VIQVRQDATP LVLAQFSAAG LEDCVAVIGQ 970 980 990 1000 1010 1020 PINNDEVSIS FHGEPVFSGQ RRLLQRQWAE TSYQIQRLRD NAECADQEFD ALLEEDNPGL 1030 1040 1050 1060 1070 1080 TVKLGFDVNE DIAAPYIKTG VRPQVAVLRE QGVNGQVEMA AAFDRAGFNA IDVHMSDILA 1090 1100 1110 1120 1130 1140 GRVDLNDFKG MVACGGFSYG DVLGAGEGWA KSALFNSRAR DAFQGFFERA DSFTLGVCNG 1150 1160 1170 1180 1190 1200 CQMLSNLHEL IPGSEFWPHF VRNRSEQFEA RVAMVQVQES ASIFLQGMAG SRMPIAIAHG 1210 1220 1230 1240 1250 1260 EGHAEFRNDD ALLEADVSGT VALRFVDNHG KVTETYPANP NGSPRGIGGM TTLDGRVTIM 1270 1280 1290 MPHPERVFRA VQNSWRPEDW NEDAAWMRMF RNARAWVN

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000058 Genomic DNA. Translation: AAZ34425.1. Different initiation.
RefSeq	YP_273599.1.
3D structure databases
HSSP	HSSP built from PDB template 1VK3 based on UniProtKB Q9X0X3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q48LX3.
Genome annotation databases
GeneID	3556978.
GenomeReviews	Gene locus PSPPH_1341 in contig CP000058_GR.
KEGG	psp:PSPPH_1341.
NMPDR	fig|264730.3.peg.1404.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HBG335309.
PhylomeDB	Q48LX3.
Family and domain databases
HAMAP	MF_00419. PurL_1. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_PSE14

Accession

Primary (citable) accession number: Q48LX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	February 9, 2010
This is version 35 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)