Reviewed, UniProtKB/Swiss-Prot Q48LC8 (MAO1_PSE14)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	PSPPH_1545

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 563	563	NAD-dependent malic enzyme HAMAP MF_01619		PRO_0000160223
Sites
	Active site	101	1	Proton donor By similarity
	Active site	172	1	Proton acceptor By similarity
	Metal binding	243	1	Divalent metal cation By similarity
	Metal binding	244	1	Divalent metal cation By similarity
	Metal binding	267	1	Divalent metal cation By similarity
	Binding site	154	1	NAD By similarity
	Binding site	267	1	NAD By similarity
	Binding site	416	1	NAD By similarity
	Site	267	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48LC8-1 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: 078CC4BA1DC8F8BE	FASTA	563	62,185
	10 20 30 40 50 60 MTKTSRPLYI SYAGPSLLEM PLLNKGSAFT PQERVEFNLI GLLPQNVETI EEQVTRVYSQ 70 80 90 100 110 120 YKQCASDLDK HIYLRSIQDN NETLFFRLLD SHLDEMLPII YTPTVGQACQ EFSKIYRTHR 130 140 150 160 170 180 GLFISYPERD RIDDILRSAT KDRIKIIVVT DSERILGLGD QGIGGMGIPI GKLSLYTACG 190 200 210 220 230 240 GISPAYTLPI VLDVGTNNRE LLDDPMYMGW RHERVSGKEY EDFIALFIDA VQRRWPDVLL 250 260 270 280 290 300 QFEDFAQSNA MPLLEKYRDE LCCFNDDIQG TASVAVGTLL AACKAKNETL GQQKVVFVGA 310 320 330 340 350 360 GSAGCGIAEH IIAAMRIEGL SESEARKRIF MVDRFGLLTE GMDNLLDFQK RLAQKTADVS 370 380 390 400 410 420 GWTAGTEAFP QLLDVVTNAG ATVLIGVSGQ RGLFTEQVIR ELHKHCAKPL VMPLSNPTSK 430 440 450 460 470 480 VEATPEEILR WTDGNALVAT GSPFAPVEIN GRTVHIAQCN NSYIFPGIGL GVVACKASRI 490 500 510 520 530 540 TDRMLMAASN ALAECSPMVT GQGDAVLPPL KEIQQVSRKI ALAVAKEAQA EGLALETSEE 550 560 ALLEAIERNF WLPGYRAYRR RSV

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000058 Genomic DNA. Translation: AAZ37192.1. Different initiation.
RefSeq	YP_273797.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3559835.
GenomeReviews	Gene locus PSPPH_1545 in contig CP000058_GR.
KEGG	psp:PSPPH_1545.
NMPDR	fig|264730.3.peg.1701.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q48LC8.
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_PSE14

Accession

Primary (citable) accession number: Q48LC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)