Q48KZ1 (TIG_PSE14) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trigger factor
Short name=TF
EC=5.2.1.8

Alternative name(s):
PPIase

Gene names

Name:	tig
Ordered Locus Names:	PSPPH_1697

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae group › Pseudomonas syringae group genomosp. 2 › Pseudomonas syringae pv. savastanoi › Pseudomonas syringae pv. phaseolicola

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase By similarity. HAMAP-Rule MF_00303
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0). HAMAP-Rule MF_00303
Subcellular location	Cytoplasm. Note: About half TF is bound to the ribosome near the polypeptide exit tunnel while the other half is free in the cytoplasm By similarity. HAMAP-Rule MF_00303
Domain	Consists of 3 domains; the N-terminus binds the ribosome, the middle domain has PPIase activity, while the C-terminus has intrinsic chaperone activity on its own By similarity. HAMAP-Rule MF_00303
Sequence similarities	Belongs to the FKBP-type PPIase family. Tig subfamily. Contains 1 PPIase FKBP-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasm
Molecular function	Chaperone Isomerase Rotamase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW protein folding Inferred from electronic annotation. Source: HAMAP protein peptidyl-prolyl isomerization Inferred from electronic annotation. Source: GOC protein transport Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	peptidyl-prolyl cis-trans isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	Trigger factor HAMAP-Rule MF_00303		PRO_0000256594
Regions
	Domain	161 – 246	86	PPIase FKBP-type

Sequences

	Sequence		Length	Mass (Da)
	Q48KZ1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 74BABA0B9C679196	FASTA	436	48,684
	10 20 30 40 50 60 MQVSVENTSA LERRMTIGVP AERIETEVNK RLQQTARKAK IPGFRPGKVP MSVIRQRYED 70 80 90 100 110 120 GARQEALGDL IQATFYEAVV EQKLNPAGAP AVEPKSFEKG KDLEYVATFE VFPEFTVAGF 130 140 150 160 170 180 DTIAVERLSA DVVDSDLDNM LEVLRKQNVR FEVADRAAQN EDQLNIDFVG KVDGEVFAGG 190 200 210 220 230 240 SATATQLVLG SGRMIPGFED GLVGAKAGEE RVLNVTFPED YQNLELAGKA AEFTVTVNTV 250 260 270 280 290 300 SEPKLPELNE EFFKQFGIKE TGIEGFRTEV RKNMERELRQ AIKSKVKNQV MDGLLAANPI 310 320 330 340 350 360 EVPKALLENE VNRLRVQAVQ QFGGNIKPDQ LPAELFEEQA KRRVELGLIV AEVVKQFDLK 370 380 390 400 410 420 PDDARVREMI QEMASAYQEP EQVVAWYYKN EQQMNEVRSV VLEEQVVDTV LQKASVTDKS 430 VSYEEAVKPV EAPKAD

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1448A / Race 6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000058 Genomic DNA. Translation: AAZ35278.1.
RefSeq	YP_273934.1. NC_005773.3.
3D structure databases
ProteinModelPortal	Q48KZ1.
ModBase	Search...
Protein-protein interaction databases
STRING	264730.PSPPH_1697.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ35278; AAZ35278; PSPPH_1697.
GeneID	3557856.
KEGG	psp:PSPPH_1697.
PATRIC	19972569. VBIPseSyr78478_1780.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0544.
HOGENOM	HOG000218239.
KO	K03545.
OMA	FPADYHG.
ProtClustDB	PRK01490.
Family and domain databases
Gene3D	1.10.3120.10. 1 hit. 3.30.70.1050. 1 hit.
HAMAP	MF_00303. Trigger_factor_Tig.
InterPro	IPR001179. PPIase_FKBP_dom. IPR005215. Trig_fac. IPR008880. Trigger_fac_C. IPR008881. Trigger_fac_ribosome-bd_bac. IPR027304. Trigger_fact/SurA_dom. [Graphical view]
Pfam	PF00254. FKBP_C. 1 hit. PF05698. Trigger_C. 1 hit. PF05697. Trigger_N. 1 hit. [Graphical view]
PIRSF	PIRSF003095. Trigger_factor. 1 hit.
SUPFAM	SSF109998. Trigger_fac_C_bac. 1 hit. SSF102735. Trigger_fac_ribosome-bd_bac. 1 hit.
TIGRFAMs	TIGR00115. tig. 1 hit.
PROSITE	PS50059. FKBP_PPIASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TIG_PSE14

Accession

Primary (citable) accession number: Q48KZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program