Q48KZ0 (CLPP_PSE14) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit
EC=3.4.21.92

Alternative name(s):
Endopeptidase Clp

Gene names

Name:	clpP
Ordered Locus Names:	PSPPH_1698

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae group › Pseudomonas syringae group genomosp. 2 › Pseudomonas syringae pv. savastanoi › Pseudomonas syringae pv. phaseolicola

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP-Rule MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP-Rule MF_00444
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00444.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	ATP-dependent Clp protease proteolytic subunit HAMAP-Rule MF_00444		PRO_0000226461
Sites
	Active site	114	1	By similarity
	Active site	139	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48KZ0 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 46D77E6981A5B206	FASTA	213	23,578
	10 20 30 40 50 60 MSRNSYIQQN SDIQAAGGLV PMVIEQSARG ERAYDIYSRL LKERVIFMVG PVEDYMANLI 70 80 90 100 110 120 AAQLLFLEAE NPDKDIHLYI NSPGGSVTAG MSIYDTMQFI KPDVSTICIG QACSMGAFLL 130 140 150 160 170 180 AGGAEGKRHC LPNSRMMIHQ PLGGFQGQAS DIDIHAKEIL HIRHRLNSLL AHHTGQSLET 190 200 210 IERDTERDNF MSAERAAEYG LIDSVINKRQ MPA

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1448A / Race 6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000058 Genomic DNA. Translation: AAZ33449.1.
RefSeq	YP_273935.1. NC_005773.3.
3D structure databases
ProteinModelPortal	Q48KZ0.
SMR	Q48KZ0. Positions 19-209.
ModBase	Search...
Protein-protein interaction databases
STRING	264730.PSPPH_1698.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ33449; AAZ33449; PSPPH_1698.
GeneID	3555975.
KEGG	psp:PSPPH_1698.
PATRIC	19972571. VBIPseSyr78478_1781.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0740.
HOGENOM	HOG000285833.
KO	K01358.
OMA	AGLIMAQ.
ProtClustDB	PRK00277.
Enzyme and pathway databases
BioCyc	PSAV264730:GKDE-1701-MONOMER.
Family and domain databases
HAMAP	MF_00444. ClpP.
InterPro	IPR001907. ClpP. IPR023562. ClpP/TepA. IPR018215. ClpP_AS. [Graphical view]
PANTHER	PTHR10381. PTHR10381. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
TIGRFAMs	TIGR00493. clpP. 1 hit.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP_PSE14

Accession

Primary (citable) accession number: Q48KZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	May 29, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program