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Q48IE2 (GLGB_PSE14) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:PSPPH_2648
OrganismPseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]
Taxonomic identifier264730 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length741 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7417411,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260679

Sites

Active site4201Nucleophile By similarity
Active site4731Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q48IE2 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: EF4650EBADF4BD54

FASTA74183,856
        10         20         30         40         50         60 
MNAPHKTGTG RRAIPAAVDI DALIRAEHRD PFSILGPHGD GGSGQYVRAY LPAALSVRLL 

        70         80         90        100        110        120 
AKDDGRELGE MEMSEVPGFF VGHLEQPQPY LLKVQWAGGE QITEDPYSFG PLLGEMDLYL 

       130        140        150        160        170        180 
FAEGNHRDLS SCLGAQVTKV DGVDGVRFAV WAPNARRVSV VGSFNSWDGR RHPMRLRHPT 

       190        200        210        220        230        240 
GVWEIFVPRL QPGEVYKYEI LGAHGILPLK SDPMALATTL PPDTASKVSA PLQFEWNDQE 

       250        260        270        280        290        300 
WLRSRTGRHD VAAPLSIYEL HAGSWQMEQH EDGQWRQYNW RELADRLIPY VKELGFTHIE 

       310        320        330        340        350        360 
LMPIMEHPFG GSWGYQLLAQ FAPTARYGSP EDFAAFVDAC HRAEIGVILD WVPAHFPTDT 

       370        380        390        400        410        420 
HGLAQFDGTA LYEYADPKEG FHQDWNTLIY NLGRTEVHGF MLASALHWLK HYHIDGLRVD 

       430        440        450        460        470        480 
AVASMLYRDY SRNAGEWVPN RFGGRENIET IDFLRHLNDV VALEAPGTMV IAEESTAWPG 

       490        500        510        520        530        540 
VSEPTQQGGL GFNYKWNMGW MHDSLQYMMR DPLHRRHHHS TLTFGLVYAW SERFVLPISH 

       550        560        570        580        590        600 
DEVVHGKHSL IDKMPGDRWQ KFANLRAYLS FMWTHPGKKL LFMGCEFGQW REWNHDEQLD 

       610        620        630        640        650        660 
WYLMQYADHV GVKNLVGDLN RLYRQEKALH QRDADPAGFQ WLIGDDQANS VFAYLRWSND 

       670        680        690        700        710        720 
GEPLLVVANM TPVPRLAYRV GAPVSGAWTE LLNSDAETYA GSNMGNGGEV NTEDEPAHGM 

       730        740 
EASLKLNLPP LAVLILKPKK D 

« Hide

References

[1]"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition."
Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. expand/collapse author list , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R.
J. Bacteriol. 187:6488-6498(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1448A / Race 6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000058 Genomic DNA. Translation: AAZ33678.1.
RefSeqYP_274839.1. NC_005773.3.

3D structure databases

ProteinModelPortalQ48IE2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264730.PSPPH_2648.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ33678; AAZ33678; PSPPH_2648.
GeneID3556214.
KEGGpsp:PSPPH_2648.
PATRIC19974605. VBIPseSyr78478_2785.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycPSAV264730:GKDE-2651-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_PSE14
AccessionPrimary (citable) accession number: Q48IE2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: September 13, 2005
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries