Reviewed, UniProtKB/Swiss-Prot Q48IB1 (DNAE2_PSE14)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	PSPPH_2680

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	1031 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: InterPro error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1031	1031	Error-prone DNA polymerase HAMAP MF_01902		PRO_1000070591

Sequences

	Sequence		Length	Mass (Da)
	Q48IB1-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: A52A908F7A4982A3	FASTA	1,031	116,205
	10 20 30 40 50 60 MTSEYAELHC LSNFSFQRGA SSARELFERA SRHGYQALAI TDECTLAGIV RAWQASISTG 70 80 90 100 110 120 LPLIIGSEMH IENGPKAVLL VESQTGYEAL CKLITVARRR ARKGEYRLLR EDFEPSSDGL 130 140 150 160 170 180 LAIWLPDIEG DAQACLAHGR WLRERFAERL WLGVELHRGA DDEQRLADLL ALAQSLGIPA 190 200 210 220 230 240 VASGDVHMHA RGRRALQDTM TAIRHHTTVA EAGHLLFANG ERHLRPLDAL AEHYPDWLLA 250 260 270 280 290 300 ESVRIARRCT FDLKQLKYEY PHELVPKGQT PTSWLRELTE RGARKRWPNG LTPATRAQVE 310 320 330 340 350 360 KELALITEKK FDSYFLTVHD IVEFARSQHI LCQGRGSAAN SAVCYALGIT ELNPEKSNLL 370 380 390 400 410 420 FERFISRERN EPPDIDVDFE HDRREEVIQY IFRRYGRGRA ALTAVASTYH GSGAMRDVAK 430 440 450 460 470 480 VLGLPPDQIN ALAEAFSRWS DSLPSPERLR EYGFDADTPI LKRVLALTGE LIGFPRHLSQ 490 500 510 520 530 540 HPGGFVISEH PLETLVPVEN AAMADRTIIQ WDKDDLDLVG LLKVDILALG MLSALRRTFD 550 560 570 580 590 600 LVHLHRGKRW TLADLPGDDR ETYEMISRAD TIGVFQIESR AQMAMLPRLR PEKFYDLVIE 610 620 630 640 650 660 VAIVRPGPIQ GDMVHPYLRR RNKEEAITYP PKLKSVFERT LGVPLFQEQV MEVAIIAAGY 670 680 690 700 710 720 TPGEADELRR AMAAWKRHGG LEPHRERLRT GMLKNGYEAD FADRIFEQIK GFGSYGFPES 730 740 750 760 770 780 HAASFALLTY ASCWLKCHEP AAFTCALINS WPMGFYSPDQ LLQDARRHHI EIRPVDVRYS 790 800 810 820 830 840 DWDCSLEPLD HPDSTRNLAI RLGLRMVRGF REDDARRIET ARSKRLFSDA TDLTLRAGLD 850 860 870 880 890 900 ARAAEALADS GALRGLIGHR HRARWEVAGV EAQRPLFNDL PSEDTQVTLP LPTVAEDLMA 910 920 930 940 950 960 DYATLGTTLG PHPLALLRRQ LAAKRFRSSQ DLLSLENDRT LSVAGLVIGR QRPGTASGVT 970 980 990 1000 1010 1020 FVTLEDEFGM VNVVVWRDLA ERQRKVLVGS QLLQVFGRLE SKSGVRHLIA QRLYDLTPLL 1030 TGLDVRSRDF Q

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000058 Genomic DNA. Translation: AAZ34765.1.
RefSeq	YP_274870.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3557327.
GenomeReviews	Gene locus PSPPH_2680 in contig CP000058_GR.
KEGG	psp:PSPPH_2680.
NMPDR	fig|264730.3.peg.2742.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q48IB1.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. PHP_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_PSE14

Accession

Primary (citable) accession number: Q48IB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 13, 2005
Last modified:	November 25, 2008
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)