Q48HJ1 (FOLD2_PSE14) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional protein FolD 2

Including the following 2 domains:

1. Methylenetetrahydrofolate dehydrogenase
   EC=1.5.1.5
2. Methenyltetrahydrofolate cyclohydrolase
   EC=3.5.4.9

Gene names

Name:	folD2
Ordered Locus Names:	PSPPH_2961

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae pv. savastanoi

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate By similarity. HAMAP MF_01576
Catalytic activity	5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH. HAMAP MF_01576 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate. HAMAP MF_01576
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01576
Subunit structure	Homodimer By similarity. HAMAP MF_01576
Sequence similarities	Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis Methionine biosynthesis One-carbon metabolism Purine biosynthesis
Ligand	NADP
Molecular function	Hydrolase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methenyltetrahydrofolate cyclohydrolase activity Inferred from electronic annotation. Source: EC methylenetetrahydrofolate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Bifunctional protein FolD 2 HAMAP MF_01576		PRO_0000268449

Sequences

	Sequence		Length	Mass (Da)
	Q48HJ1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 15B97AB553840938	FASTA	300	31,451
	10 20 30 40 50 60 MSARIIDGKA AAARVLEQVS TEVKILKADG IEPALAVILV GNDPASEVYV RNKILRAEEA 70 80 90 100 110 120 GIRSLEHRLP AKSSQAQVLA LIAELNADDS VNGILLQLPL PSHMEEARAL QAIDPGKDVD 130 140 150 160 170 180 GFHSENVGGL SQGRSVLTPC TPSGCMHLLE ETCGDLSGKH AVVIGRSNIV GKPMAALLLQ 190 200 210 220 230 240 AHCSVTVVHS RSTDAKALCQ LADIVVAAVG RPRMIDASWL KPGAVVIDVG INRIEDQGRS 250 260 270 280 290 300 RLVGDIDFDN ALSVASAITP VPGGVGPMTI AFLMKNTVTA ARQQALAQRS QSEAVCLSTC

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1448A / Race 6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000058 Genomic DNA. Translation: AAZ37909.1.
RefSeq	YP_275142.1. NC_005773.3.
3D structure databases
HSSP	HSSP built from PDB template 2C2X based on UniProtKB O50385.
ProteinModelPortal	Q48HJ1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q48HJ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3560579.
GenomeReviews	Gene locus PSPPH_2961 in contig CP000058_GR.
KEGG	psp:PSPPH_2961.
NMPDR	fig|264730.3.peg.3027.
PATRIC	19975253. VBIPseSyr78478_3108.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0190.
HOGENOM	HBG328751.
OMA	QTVEVGM.
PhylomeDB	Q48HJ1.
ProtClustDB	PRK14194.
Family and domain databases
HAMAP	MF_01576. THF_DHG_CYH. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR000672. THF_DH/CycHdrlase. IPR020630. THF_DH/CycHdrlase_cat_dom. IPR020867. THF_DH/CycHdrlase_CS. IPR020631. THF_DH/CycHdrlase_NAD-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01491.
Pfam	PF00763. THF_DHG_CYH. 1 hit. PF02882. THF_DHG_CYH_C. 1 hit. [Graphical view]
PRINTS	PR00085. THFDHDRGNASE.
PROSITE	PS00766. THF_DHG_CYH_1. 1 hit. PS00767. THF_DHG_CYH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FOLD2_PSE14

Accession

Primary (citable) accession number: Q48HJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program