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Q48GW3

- FADB_PSE14

UniProt

Q48GW3 - FADB_PSE14

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Protein
Fatty acid oxidation complex subunit alpha
Gene
fadB, PSPPH_3210
Organism
Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate By similarity.UniRule annotation

Catalytic activityi

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation
(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei120 – 1201Important for catalytic activity By similarity
Sitei140 – 1401Important for catalytic activity By similarity
Binding sitei297 – 2971Substrate By similarity
Binding sitei325 – 3251NAD; via amide nitrogen By similarity
Binding sitei344 – 3441NAD By similarity
Binding sitei408 – 4081NAD By similarity
Active sitei451 – 4511For 3-hydroxyacyl-CoA dehydrogenase activity By similarity
Binding sitei454 – 4541NAD By similarity
Binding sitei501 – 5011Substrate By similarity
Binding sitei660 – 6601Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi401 – 4033NAD By similarity
Nucleotide bindingi428 – 4303NAD By similarity

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydrogenase activity Source: JCVI
  2. 3-hydroxybutyryl-CoA epimerase activity Source: JCVI
  3. coenzyme binding Source: InterPro
  4. dodecenoyl-CoA delta-isomerase activity Source: JCVI
  5. enoyl-CoA hydratase activity Source: JCVI

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB-UniPathway
  2. fatty acid catabolic process Source: JCVI
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciPSAV264730:GKDE-3213-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alpha
Including the following 2 domains:
Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3, EC:5.3.3.8)
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
Gene namesi
Name:fadB
Ordered Locus Names:PSPPH_3210
OrganismiPseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)
Taxonomic identifieri264730 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000551: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. fatty acid beta-oxidation multienzyme complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Fatty acid oxidation complex subunit alphaUniRule annotation
PRO_0000109280Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA) By similarity.

Protein-protein interaction databases

STRINGi264730.PSPPH_3210.

Structurei

3D structure databases

ProteinModelPortaliQ48GW3.
SMRiQ48GW3. Positions 1-715.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 190190Enoyl-CoA hydratase/isomerase By similarity
Add
BLAST
Regioni312 – 7214103-hydroxyacyl-CoA dehydrogenase By similarity
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261344.
KOiK01825.
OMAiNPIVVND.
OrthoDBiEOG6M9F0M.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01621. FadB.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012799. FadB.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02437. FadB. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q48GW3-1 [UniParc]FASTAAdd to Basket

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MIYEGKAITV KALESGIVEL NFDLKGESVN KFNRLTLNEL RQAVDAIKAD    50
ASVKGVIVSS GKDVFIVGAD ITEFVDNFKL PEAELVAGNL QANRIFSDFE 100
DLGVPTVVAI NGIALGGGLE MCLAADYRVI SSSARIGLPE VKLGLYPGFG 150
GTVRLPRIIG ADNAIEWIAS GKESSAEDAL KVGAVDAVVA PEKLQAAALD 200
LIQRAISGEF DYKAKRQPKL DKLKLNAIEQ MMAFETAKGF VAGQAGPNYP 250
APVEAIKTIQ KAANFGRDKA LEIEAAGFVK MAKTSAAQSL IGLFLNDQEL 300
KKKAKGYDAV AKDVKQAAVL GAGIMGGGIA YQSAVKGTPI LMKDIREEAI 350
QLGLNEASKL LGGRLEKGRL TAAKMAEALN AIRPTLSYGD FGNVDLVVEA 400
VVENPKVKQA VLAEVEANVG EHTILASNTS TISISLLAKA LKRPENFVGM 450
HFFNPVHMMP LVEVIRGEKS SEEAVATTVA YARKMGKNPI VVNDCPGFLV 500
NRVLFPYFGG FARLVSAGVD FVRIDKVMEK FGWPMGPAYL MDVVGIDTGH 550
HGRDVMAEGF PDRMKDDRRS VVDALYEAKR LGQKNGKGFY AYETDKKGKP 600
KKVNDPAVLD VLKPIVYEQR EVSDEDIINW MMIPLCLETV RCLEDGIVET 650
AAEADMGLIY GIGFPPFRGG ALRYIDSIGV AEFVALADQY AELGALYQPT 700
AKLREMASKG QSFFGQASSE E 721
Length:721
Mass (Da):77,630
Last modified:September 13, 2005 - v1
Checksum:iC66C6B34D3EFFC89
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000058 Genomic DNA. Translation: AAZ34330.1.
RefSeqiYP_275370.1. NC_005773.3.

Genome annotation databases

EnsemblBacteriaiAAZ34330; AAZ34330; PSPPH_3210.
GeneIDi3556880.
KEGGipsp:PSPPH_3210.
PATRICi19975810. VBIPseSyr78478_3377.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000058 Genomic DNA. Translation: AAZ34330.1 .
RefSeqi YP_275370.1. NC_005773.3.

3D structure databases

ProteinModelPortali Q48GW3.
SMRi Q48GW3. Positions 1-715.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264730.PSPPH_3210.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ34330 ; AAZ34330 ; PSPPH_3210 .
GeneIDi 3556880.
KEGGi psp:PSPPH_3210.
PATRICi 19975810. VBIPseSyr78478_3377.

Phylogenomic databases

eggNOGi COG1250.
HOGENOMi HOG000261344.
KOi K01825.
OMAi NPIVVND.
OrthoDBi EOG6M9F0M.

Enzyme and pathway databases

UniPathwayi UPA00659 .
BioCyci PSAV264730:GKDE-3213-MONOMER.

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPi MF_01621. FadB.
InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012799. FadB.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsi TIGR02437. FadB. 1 hit.
PROSITEi PS00067. 3HCDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1448A / Race 6.

Entry informationi

Entry nameiFADB_PSE14
AccessioniPrimary (citable) accession number: Q48GW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: September 13, 2005
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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