Q48GS7 (HGD_PSE14) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homogentisate 1,2-dioxygenase
EC=1.13.11.5

Alternative name(s):
Homogentisate oxygenase
Homogentisic acid oxidase
Homogentisicase

Gene names

Name:	hmgA
Ordered Locus Names:	PSPPH_3246

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae pv. savastanoi

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP MF_00334
Cofactor	Iron By similarity. HAMAP MF_00334
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Homogentisate 1,2-dioxygenase HAMAP MF_00334		PRO_0000225793
Sites
	Metal binding	332	1	Iron By similarity
	Metal binding	338	1	Iron By similarity
	Metal binding	368	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48GS7 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 780C40AF83FC95C0	FASTA	435	48,438
	10 20 30 40 50 60 MAIHSRSDAL AYQSGFGNQF SSEALPGALP IGQNSPQKHP LGLYAEQFSG TAFTVARSEA 70 80 90 100 110 120 RRTWLYRIKP SAAHPRYQRM GRQIAGQEQG PINPNRLRWN AFDIPAEPVD FIDGLIPLAS 130 140 150 160 170 180 TSAADQADGV SVYVYAANTS MQRAFFSADG EWLIVPQQGR LRIITELGLL DIEPLEIAVL 190 200 210 220 230 240 PRGMKFRVQL LDSSARGYIC ENHGCALRLP ELGPIGSNGL ANPRDFLTPV AWFEDNQQPV 250 260 270 280 290 300 ELVQKFLGEL WSTHLEHSPF DVVGWHGNNV AYKYDLRRFN TIGTVSYDHP DPSIFTVLTS 310 320 330 340 350 360 PGAAHGQANI DFVIFPPRWM VAENTFRPPW FHRNLMNEFM GLIDGAYDAK AEGFMPGGAS 370 380 390 400 410 420 LHNCMSAHGP DNVTAEKAIA AELKPHKIEN TMAFMFETGK VLRPSRHALG CPQLQADYDA 430 CWNDMTRTFN KEPRR

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1448A / Race 6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000058 Genomic DNA. Translation: AAZ36498.1.
RefSeq	YP_275406.1. NC_005773.3.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q48GS7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3559119.
GenomeReviews	Gene locus PSPPH_3246 in contig CP000058_GR.
KEGG	psp:PSPPH_3246.
NMPDR	fig|264730.3.peg.3236.
PATRIC	19975884. VBIPseSyr78478_3414.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3508.
HOGENOM	HBG293508.
OMA	RNCMSEF.
ProtClustDB	PRK05341.
Family and domain databases
HAMAP	MF_00334. Homogentis_dioxygen. [Tree]
InterPro	IPR011051. Cupin_RmlC_type. IPR005708. Homogentis_dOase. IPR022950. Homogentis_dOase_bac. [Graphical view]
KO	K00451.
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01015. HmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_PSE14

Accession

Primary (citable) accession number: Q48GS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program