Reviewed, UniProtKB/Swiss-Prot Q48G19 (ASTD_PSE14)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	PSPPH_3518

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD(+) + H(2)O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. HAMAP MF_01174
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174		PRO_0000262417
Regions
	Nucleotide binding	221 – 226	6	NAD By similarity
Sites
	Active site	244	1	By similarity
	Active site	278	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48G19-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: D14DE7FC1E31F294	FASTA	488	51,455
	10 20 30 40 50 60 MSTLYIAGAW QAGQGELFHS LNPVSQQTLW SGQAATPEQV DYAVQAARQA FPGWAQRSLD 70 80 90 100 110 120 QRIAVLEAFA ASLKGRADEL AHCIGEETGK PLWESATEVT SMVNKIAISV QSYRERTGEK 130 140 150 160 170 180 SGPLGDATAV LRHKPHGVVA VFGPYNFPGH LPNGHIVPAL LAGNTVVFKP SELTPKVAEL 190 200 210 220 230 240 TVKCWIEAGL PAGVLNLLQG GRETGIALAA NPGIDGLFFT GSSRTGDALH QQFAGRPDKI 250 260 270 280 290 300 LALEMGGNNP LIVDQVQDIE AAVYNIIQSA FISAGQRCTC ARRLLVPEGD WGDALLARLV 310 320 330 340 350 360 AVSATIEVGA FDQQPSPFMG SVISLEAAHA LLDAQRNLLA NGAVTLLEMR QPQAGAALLT 370 380 390 400 410 420 PGIINVSAVA ERPDEELFGP LLQVIRYAGF DAAIAEANAT RYGLAAGLLS DSEARYQQFW 430 440 450 460 470 480 LHSRAGIVNW NKQLTGAASS APFGGVGASG NHRASAYYAA DYCAYPVASL EAGSLTLPST LTPGIRLS

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000058 Genomic DNA. Translation: AAZ37796.1.
RefSeq	YP_275666.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3560463.
GenomeReviews	Gene locus PSPPH_3518 in contig CP000058_GR.
KEGG	psp:PSPPH_3518.
NMPDR	fig|264730.3.peg.3417.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q48G19.
Family and domain databases
HAMAP	MF_01174. [Tree]
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_PSE14

Accession

Primary (citable) accession number: Q48G19

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	September 13, 2005
Last modified:	November 25, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)