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Q48F57

- GLND_PSE14

UniProt

Q48F57 - GLND_PSE14

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene
glnD, PSPPH_3842
Organism
Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPSAV264730:GKDE-3845-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:PSPPH_3842
OrganismiPseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)
Taxonomic identifieri264730 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000551: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 898898Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotationPRO_0000231687Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264730.PSPPH_3842.

Structurei

3D structure databases

ProteinModelPortaliQ48F57.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini463 – 567105HDAdd
BLAST
Domaini705 – 78379ACT 1Add
BLAST
Domaini815 – 89177ACT 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 341341UridylyltransferaseUniRule annotationAdd
BLAST
Regioni342 – 704363Uridylyl-removingUniRule annotationAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q48F57-1 [UniParc]FASTAAdd to Basket

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MPQVDPDLFD RGQFQAELAL KASPIAAFKK AIRRAKDVLD DRFKSGRDIR    50
RLIEDRAWFV DNILQKAWDQ FEWSEDADIA LLAVGGYGRG ELHPYSDIDL 100
LILLDSDDHE VFREPIERFL TLLWDIGLEV GQSVRSVNEC AQEGRADLTV 150
ITNLMESRTI AGPEHLRQRM LEVTSTEHMW PSKEFFLAKH AEQKKRHHKY 200
NDTEYNLEPN VKGSPGGLRD IQTILWVARR QYGTLNLHAL AGEGFLLGSE 250
NALLASSQEF LWKVRYALHM LAGRSEDRLL FDYQVRIAGL LGYEDSDAKL 300
AIERFMQKYY RVVMSIAELS DLIIQHFEEV ILSDDSGTAQ PINSRFQLHD 350
GYIEATNPNV FKRTPFAMIE IFVLMAQHPE IKGVRADTIR LLREHRHLIN 400
DDFRNDIRNT SLFIELFKCE TGIHRNLRRM NRYGILGLYL PEFGHIVGQM 450
QHDLFHIYTV DAHTLNLIKH LRKLQYTEVS EKFPLASKIM ARLPKPELIY 500
LAGLYHDIGK GRGGDHSELG AIDAQAFGTR HHLPDWDTRL IVWLVSNHLV 550
MSTTAQRKDL SDPQVIHDFA QFVGDEVHLD YLYVLTVADI NATNPTLWNS 600
WRASLLRQLY TETKRALRRG LENPVDREEQ IRRTQTAALD ILVRNGTDPD 650
DVEQLWSALG DDYFLRHTAG DVAWHSDAIL QQPADGGPLV LIKETTQREF 700
EGGTQIFIYA PDQHDFFAVT VAAMDQLNLN IHDARIITSS SKFTLDTYIV 750
LDNEGGSIGD NPERVQEIRN GLTEALRNPD DYPTIIKRRV PRQLKHFAFA 800
PQVTIHNDAQ RPVTVLELLA PDRPGLLARI GKIFLEFDLS LQNAKIATLG 850
ERVEDVFFIT DANNHPLSDP QLCSQLQDAI VKQLSVNSEP GHDLRISI 898
Length:898
Mass (Da):102,672
Last modified:September 13, 2005 - v1
Checksum:i0F0D33C6388E4BD4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000058 Genomic DNA. Translation: AAZ35473.1.
RefSeqiWP_004667439.1. NC_005773.3.
YP_275980.1. NC_005773.3.

Genome annotation databases

EnsemblBacteriaiAAZ35473; AAZ35473; PSPPH_3842.
GeneIDi3558061.
KEGGipsp:PSPPH_3842.
PATRICi19977127. VBIPseSyr78478_4024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000058 Genomic DNA. Translation: AAZ35473.1 .
RefSeqi WP_004667439.1. NC_005773.3.
YP_275980.1. NC_005773.3.

3D structure databases

ProteinModelPortali Q48F57.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264730.PSPPH_3842.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ35473 ; AAZ35473 ; PSPPH_3842 .
GeneIDi 3558061.
KEGGi psp:PSPPH_3842.
PATRICi 19977127. VBIPseSyr78478_4024.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci PSAV264730:GKDE-3845-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1448A / Race 6.

Entry informationi

Entry nameiGLND_PSE14
AccessioniPrimary (citable) accession number: Q48F57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: September 13, 2005
Last modified: September 3, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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