Reviewed, UniProtKB/Swiss-Prot Q48F46 (PLSB_PSE14)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	PSPPH_3853

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae pv. savastanoi

Protein attributes

Sequence length	833 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 833	833	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049440
Regions
	Motif	309 – 314	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	Q48F46-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 59081D4F9772858B	FASTA	833	94,470
	10 20 30 40 50 60 MTRSPFRRLV FGTLRRLLYL WVRSETINQS SFTLNLDRSR PVFYALQSPS ISDLAVIDTE 70 80 90 100 110 120 CRKAGLPRPV LSVAVGNLIE PAAFFYLTPA PDWLGRQDKR GAPPTLERLV AAVSQNPGED 130 140 150 160 170 180 AQIIPVSVFW GQSPDRESSA WKLLFADSWA VTGRLRRLVS ILILGRKTRV QFSAPIHMRE 190 200 210 220 230 240 LVGENKGYEL TLRMTQRLLR VHFRNLKSAV IGPDVSHRRT VVKGLLDEPL VKQAIIEEAE 250 260 270 280 290 300 RENITQDKAR DRALSYGNEI ASDYTYSVIR FMEVVLSWFW NKIYDGIKVS HIEGVQEVAP 310 320 330 340 350 360 GHEVIYVPCH RSHIDYLLLS YLLFCNGLTP PHIAAGINLN MPVVGSLLRR GGAFFMRRTF 370 380 390 400 410 420 KGNPLYTAVF TEYLHTLFIK GFPVEYFVEG GRSRTGRMLQ PKTGMLAITL RSFLRNSRMP 430 440 450 460 470 480 IVFVPLYIGY ERVLEGRTYL GELRGATKKK ESIFDIFKVI GALKQRFGQV SVNFGAPIKL 490 500 510 520 530 540 AEFLDGEQPD WREQALAPQF RPEWLSETTH RLGERVAQHL NEAAAVNPMN LVAVALLSTQ 550 560 570 580 590 600 RLALDDQAME RVLDLYLTLL RAVPYSPHTT LPEGDGRSLI EHVKGMDLLA EQKDALGKIL 610 620 630 640 650 660 YLNEQNAVLM TYYRNNVLHI FALPSLLASF FQSSSRMSRE QILRYTRALY PFLQSELFIR 670 680 690 700 710 720 WPLSELDEVV DQWLAAFVEQ GLLRFKKDVY VRPEPSSREF VLLTLLSRAI AQTLQRFYMA 730 740 750 760 770 780 IALLLNSGQN TLSAEQLEDL CTVMAQRLSI LHGLNAPEFF DKSLFRHFIQ TLLDLGVLRK 790 800 810 820 830 DSAGKLSYHP MLGELAEGAA KRVLPAEIRL SIRQVALHSN EEEQDAGNGE GVA

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000058 Genomic DNA. Translation: AAZ33585.1.
RefSeq	YP_275991.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q48F46.
Genome annotation databases
GeneID	3556116.
GenomeReviews	Gene locus PSPPH_3853 in contig CP000058_GR.
KEGG	psp:PSPPH_3853.
NMPDR	fig|264730.3.peg.4096.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2937.
HOGENOM	HBG296590.
OMA	WNKLYQG.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_PSE14

Accession

Primary (citable) accession number: Q48F46

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	January 19, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)