Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycerol-3-phosphate acyltransferase

Gene

plsB

Organism
Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6) (Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.UniRule annotation

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase (plsB)
  2. no protein annotated in this organism
  3. Phosphatidate cytidylyltransferase (cdsA)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciPSAV264730:GKDE-3856-MONOMER.
UniPathwayiUPA00557; UER00612.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate acyltransferaseUniRule annotation (EC:2.3.1.15UniRule annotation)
Short name:
GPATUniRule annotation
Gene namesi
Name:plsBUniRule annotation
Ordered Locus Names:PSPPH_3853
OrganismiPseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6) (Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6))
Taxonomic identifieri264730 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000551 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 833833Glycerol-3-phosphate acyltransferasePRO_1000049440Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264730.PSPPH_3853.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi309 – 3146HXXXXD motif

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.UniRule annotation

Sequence similaritiesi

Belongs to the GPAT/DAPAT family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E55. Bacteria.
COG2937. LUCA.
HOGENOMiHOG000218231.
KOiK00631.
OMAiEVIYVPC.
OrthoDBiEOG6B625B.

Family and domain databases

HAMAPiMF_00393. Glyc3P_acyltrans.
InterProiIPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 2 hits.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03703. plsB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q48F46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRSPFRRLV FGTLRRLLYL WVRSETINQS SFTLNLDRSR PVFYALQSPS
60 70 80 90 100
ISDLAVIDTE CRKAGLPRPV LSVAVGNLIE PAAFFYLTPA PDWLGRQDKR
110 120 130 140 150
GAPPTLERLV AAVSQNPGED AQIIPVSVFW GQSPDRESSA WKLLFADSWA
160 170 180 190 200
VTGRLRRLVS ILILGRKTRV QFSAPIHMRE LVGENKGYEL TLRMTQRLLR
210 220 230 240 250
VHFRNLKSAV IGPDVSHRRT VVKGLLDEPL VKQAIIEEAE RENITQDKAR
260 270 280 290 300
DRALSYGNEI ASDYTYSVIR FMEVVLSWFW NKIYDGIKVS HIEGVQEVAP
310 320 330 340 350
GHEVIYVPCH RSHIDYLLLS YLLFCNGLTP PHIAAGINLN MPVVGSLLRR
360 370 380 390 400
GGAFFMRRTF KGNPLYTAVF TEYLHTLFIK GFPVEYFVEG GRSRTGRMLQ
410 420 430 440 450
PKTGMLAITL RSFLRNSRMP IVFVPLYIGY ERVLEGRTYL GELRGATKKK
460 470 480 490 500
ESIFDIFKVI GALKQRFGQV SVNFGAPIKL AEFLDGEQPD WREQALAPQF
510 520 530 540 550
RPEWLSETTH RLGERVAQHL NEAAAVNPMN LVAVALLSTQ RLALDDQAME
560 570 580 590 600
RVLDLYLTLL RAVPYSPHTT LPEGDGRSLI EHVKGMDLLA EQKDALGKIL
610 620 630 640 650
YLNEQNAVLM TYYRNNVLHI FALPSLLASF FQSSSRMSRE QILRYTRALY
660 670 680 690 700
PFLQSELFIR WPLSELDEVV DQWLAAFVEQ GLLRFKKDVY VRPEPSSREF
710 720 730 740 750
VLLTLLSRAI AQTLQRFYMA IALLLNSGQN TLSAEQLEDL CTVMAQRLSI
760 770 780 790 800
LHGLNAPEFF DKSLFRHFIQ TLLDLGVLRK DSAGKLSYHP MLGELAEGAA
810 820 830
KRVLPAEIRL SIRQVALHSN EEEQDAGNGE GVA
Length:833
Mass (Da):94,470
Last modified:September 13, 2005 - v1
Checksum:i59081D4F9772858B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000058 Genomic DNA. Translation: AAZ33585.1.
RefSeqiWP_011169304.1. NC_005773.3.

Genome annotation databases

EnsemblBacteriaiAAZ33585; AAZ33585; PSPPH_3853.
KEGGipsp:PSPPH_3853.
PATRICi19977149. VBIPseSyr78478_4035.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000058 Genomic DNA. Translation: AAZ33585.1.
RefSeqiWP_011169304.1. NC_005773.3.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264730.PSPPH_3853.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ33585; AAZ33585; PSPPH_3853.
KEGGipsp:PSPPH_3853.
PATRICi19977149. VBIPseSyr78478_4035.

Phylogenomic databases

eggNOGiENOG4105E55. Bacteria.
COG2937. LUCA.
HOGENOMiHOG000218231.
KOiK00631.
OMAiEVIYVPC.
OrthoDBiEOG6B625B.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00612.
BioCyciPSAV264730:GKDE-3856-MONOMER.

Family and domain databases

HAMAPiMF_00393. Glyc3P_acyltrans.
InterProiIPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 2 hits.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03703. plsB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1448A / Race 6.

Entry informationi

Entry nameiPLSB_PSE14
AccessioniPrimary (citable) accession number: Q48F46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: November 11, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.