Reviewed, UniProtKB/Swiss-Prot Q48ED3 (CYSD_PSE14)

Names and origin

Protein names

Recommended name:
    Sulfate adenylyltransferase subunit 2
    EC=2.7.7.4
Alternative name(s):
    Sulfate adenylate transferase
      Short name=SAT
    ATP-sulfurylase small subunit

Gene names

Name:	cysD
Ordered Locus Names:	PSPPH_4133

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of cysD, the smaller subunit, and cysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate assimilation Inferred from electronic annotation. Source: HAMAP sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_1000008965

Sequences

	Sequence		Length	Mass (Da)
	Q48ED3-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 93CE76EA132C89C8	FASTA	305	35,312
	10 20 30 40 50 60 MVDKLTHLKQ LEAESIHIIR EVAAEFDNPV MLYSIGKDSA VMLHLARKAF FPGKLPFPVM 70 80 90 100 110 120 HVDTRWKFQE MYRFRDKMVE EMGLDLITHI NPDGVAQGIN PFTHGSAKHT DIMKTEGLKQ 130 140 150 160 170 180 ALDKHGFDAA FGGARRDEEK SRAKERVYSF RDSKHRWDPK NQRPELWNVY NGNVNKGESI 190 200 210 220 230 240 RVFPLSNWTE LDIWQYIYLE GIPIVPLYFA AERDVIEKNG TLIMIDDERI LEHLTDEEKS 250 260 270 280 290 300 RIVKKKVRFR TLGCYPLTGA VESEATSLTD IIQEMLLTRT SERQGRVIDH DGAGSMEEKK RQGYF

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000058 Genomic DNA. Translation: AAZ35686.1.
RefSeq	YP_276255.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3558279.
GenomeReviews	Gene locus PSPPH_4133 in contig CP000058_GR.
KEGG	psp:PSPPH_4133.
NMPDR	fig|264730.3.peg.4531.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q48ED3.
OMA	Q48ED3. SLRVFPL.
Family and domain databases
HAMAP	MF_00064. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_PSE14

Accession

Primary (citable) accession number: Q48ED3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)