Reviewed, UniProtKB/Swiss-Prot Q48DU7 (GLYA1_PSE14)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 1
      Short name=Serine methylase 1
      Short name=SHMT 1
    EC=2.1.2.1

Gene names

Name:	glyA1
Ordered Locus Names:	PSPPH_4327

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae pv. savastanoi

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Serine hydroxymethyltransferase 1 HAMAP MF_00051		PRO_0000235007
Amino acid modifications
	Modified residue	229	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48DU7-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 5C1C1960BF14A789	FASTA	417	44,844
	10 20 30 40 50 60 MFSRDLTIAK YDADLFAAME QEALRQEEHI ELIASENYTS PAVMEAQGSA LTNKYAEGYP 70 80 90 100 110 120 GKRYYGGCEY VDVIEQLAID RAKELFGADY ANVQPHAGSQ ANSAVYLALL QGGDTILGMS 130 140 150 160 170 180 LAHGGHLTHG ASVSSSGKLY NAVQYGIDAN GMIDYDEVER LAVEHKPKMI VAGFSAYSQI 190 200 210 220 230 240 LDFPRFRAIA DKVGAYLFVD MAHVAGLVAA GVYPNPVPFA DVVTTTTHKT LRGPRGGLIL 250 260 270 280 290 300 ARANAEIEKK LNSAVFPGSQ GGPLEHVIAA KAVCFKEALQ PEFKTYQQQV VKNAKAMAGV 310 320 330 340 350 360 FIERGFDVVS GGTENHLFLL SLIKQDISGK DADAALGRAF ITVNKNSVPN DPRSPFVTSG 370 380 390 400 410 LRFGTPAVTT RGFKEAECKE LAGWICDILA DLNNEAVIDA VREKVKAICA KLPVYGA

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000058 Genomic DNA. Translation: AAZ36341.1.
RefSeq	YP_276444.1.
3D structure databases
SMR	Q48DU7. Positions 1-416.
ModBase	Search...
Protein-protein interaction databases
STRING	Q48DU7.
Genome annotation databases
GeneID	3558958.
GenomeReviews	Gene locus PSPPH_4327 in contig CP000058_GR.
KEGG	psp:PSPPH_4327.
NMPDR	fig|264730.3.peg.3743.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q48DU7.
OMA	NNAKAMV.
Family and domain databases
HAMAP	MF_00051. [Tree]
InterPro	IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA1_PSE14

Accession

Primary (citable) accession number: Q48DU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	September 13, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)