Q48DT7 (RLMG_PSE14) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	PSPPH_4337

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae pv. savastanoi

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.
Sequence caution	The sequence AAZ36436.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366485

Sequences

	Sequence		Length	Mass (Da)
	Q48DT7 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: DB5EDA67617EF282	FASTA	374	40,697
	10 20 30 40 50 60 MPLLISPFAE LDLIRQPEQQ DEPLQAFDAA DEYLLNHVAE TGLSLQSRVL VLNDSFGALA 70 80 90 100 110 120 ASLASHATVV SSTDSFLAAQ GLEKNLARNG MSYDAVPHIP ASEPLSGPFD WVLIRVPKTL 130 140 150 160 170 180 ALLEEQLIRL QGQLAPGARV VAAAMVKHLP RSAGDLLEEY VGPVQASLAV KKARLLFATP 190 200 210 220 230 240 QPMEVRTSPY PTRYRLDEPA IELLNHANVF CRDGLDIGTR AFLPYLPKNL GTARVADLGC 250 260 270 280 290 300 GNGVLAIASA LDNPQAHYTL VDESFMAVQS AAENWRATLG ERVVEVRAAD GLDTQEPDSL 310 320 330 340 350 360 DVVLCNPPFH QQQVVGDFLA WRMFLQARAA LVNGGALYIV GNRHLGYHTK LSRLFRGVEQ 370 VAATPKFVIL KARK

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1448A / Race 6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000058 Genomic DNA. Translation: AAZ36436.1. Different initiation.
RefSeq	YP_276454.1. NC_005773.3.
3D structure databases
ProteinModelPortal	Q48DT7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q48DT7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3559056.
GenomeReviews	Gene locus PSPPH_4337 in contig CP000058_GR.
KEGG	psp:PSPPH_4337.
NMPDR	fig|264730.3.peg.3828.
PATRIC	19978157. VBIPseSyr78478_4535.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
ProtClustDB	CLSK868782.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K00564.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_PSE14

Accession

Primary (citable) accession number: Q48DT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program