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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6) (Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei630 – 6301ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPSAV264730:GKDE-4398-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:PSPPH_4394
OrganismiPseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6) (Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6))
Taxonomic identifieri264730 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000551 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 868868Leucine--tRNA ligasePRO_1000009396Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264730.PSPPH_4394.

Structurei

3D structure databases

ProteinModelPortaliQ48DN1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi627 – 6315"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q48DN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHELYQPREI EAAAQTFWDE QKSFEVSEQP GKDTFYCLSM FPYPSGKLHM
60 70 80 90 100
GHVRNYTIGD VISRYQRMLG KNVLQPLGWD AFGMPAENAA IDNNVAPAKW
110 120 130 140 150
TYENIAYMKN QLKSLGLAVD WSREVTTCKP DYYRWEQWLF TRLFEKGVIY
160 170 180 190 200
RKNGTVNWDP VDQTVLANEQ VIDGRGWRSG ALIEKREIPM YYFKITAYAD
210 220 230 240 250
ELLESLDELP GWPEQVKTMQ RNWIGRSRGM EVQFPYDQAS IGEAGALKVF
260 270 280 290 300
TTRPDTLMGA TYVAVAAEHP LATLAAQGNP GLQAFIDECK GGSVAEADVA
310 320 330 340 350
TQEKKGQPTS LFVEHPLTGE KLPVWVANYV LMHYGDGAVM AVPAHDERDF
360 370 380 390 400
EFATHYGLPI KPVVRTSAGD QTPAPWQPAY GEHGELINSG EFTGLNFQAA
410 420 430 440 450
FDAIEAALVK KSLGQSRTQF RLRDWGISRQ RYWGCPIPIV HCDTCGDVPV
460 470 480 490 500
PEDQLPVVLP EDVVPDGAGS PLARMPEFYE CSCPKCGAPA KRETDTMDTF
510 520 530 540 550
VESSWYYARY ASPHYEGGLV EPNAANHWLP VDQYIGGIEH AILHLLYARF
560 570 580 590 600
FHKLMRDEGL VTSNEPFKNL LTQGMVNAET YFRMETSGKK TWINPADVTL
610 620 630 640 650
ERDAKAKVIS AKLTSDGLPV EIGGTEKMSK SKKNGIDPQT MIDQYGADTC
660 670 680 690 700
RLFMMFASPP DMSLEWSDSG VEGSHRFLRR VWRLAQAHVA QGASTGLDIA
710 720 730 740 750
ALTDDQKTIR RSIHQAIRQA SQDIGQNQKF NTAVAQVMTL MNVLEKAPQV
760 770 780 790 800
TPQDRALLQE GLETVTLLLA PITPHISHEL WTQLGHNEPV IDAGWPAFDA
810 820 830 840 850
NALVQDSLQL VIQVNGKLRG HIEMPASASR EEVEAAARIN ENVLRFTDGL
860
TIRKVIVVPG KLVNIVAS
Length:868
Mass (Da):96,868
Last modified:September 13, 2005 - v1
Checksum:i6A74904B9092EE52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000058 Genomic DNA. Translation: AAZ36139.1.
RefSeqiWP_011169541.1. NC_005773.3.
YP_276510.1. NC_005773.3.

Genome annotation databases

EnsemblBacteriaiAAZ36139; AAZ36139; PSPPH_4394.
KEGGipsp:PSPPH_4394.
PATRICi19978277. VBIPseSyr78478_4595.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000058 Genomic DNA. Translation: AAZ36139.1.
RefSeqiWP_011169541.1. NC_005773.3.
YP_276510.1. NC_005773.3.

3D structure databases

ProteinModelPortaliQ48DN1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264730.PSPPH_4394.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ36139; AAZ36139; PSPPH_4394.
KEGGipsp:PSPPH_4394.
PATRICi19978277. VBIPseSyr78478_4595.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciPSAV264730:GKDE-4398-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1448A / Race 6.

Entry informationi

Entry nameiSYL_PSE14
AccessioniPrimary (citable) accession number: Q48DN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: May 27, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.