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Q48DM6

- LIPA_PSE14

UniProt

Q48DM6 - LIPA_PSE14

Protein

Lipoyl synthase

Gene

lipA

Organism
Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi81 – 811Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi92 – 921Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi107 – 1071Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi111 – 1111Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi114 – 1141Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPSAV264730:GKDE-4403-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:PSPPH_4399
    OrganismiPseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)
    Taxonomic identifieri264730 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000000551: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 335335Lipoyl synthasePRO_0000325292Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi264730.PSPPH_4399.

    Structurei

    3D structure databases

    ProteinModelPortaliQ48DM6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiHPHIPTK.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q48DM6-1 [UniParc]FASTAAdd to Basket

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    MTDTVQTLIP TLDVSERVAR PKVEAGVKLR GAEKVARIPV KIIPTVDLPK    50
    KPDWIRVRIP VSPEVDRIKQ LLRKHKLHSV CEEASCPNLG ECFSGGTATF 100
    MIMGDICTRR CPFCDVGHGR PKPLDVNEPK SLAIAIADLR LKYVVITSVD 150
    RDDLRDGGAQ HFADCIREIR LLSPGVQLET LVPDYRGRMD VALEITAAEP 200
    PDVFNHNLET VPRLYKAARP GSDYQWSLTL LQRFKQMVPH VPTKSGLMLG 250
    LGETDEEVIE VMQRMREHDI DMLTLGQYLQ PSRNHLPVQR FVHPDTFAWF 300
    AEEGYKMGFK NVASGPLVRS SYHADEQAKI AKAML 335
    Length:335
    Mass (Da):37,665
    Last modified:September 13, 2005 - v1
    Checksum:i198B58FE2D1E004A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000058 Genomic DNA. Translation: AAZ35196.1.
    RefSeqiYP_276515.1. NC_005773.3.

    Genome annotation databases

    EnsemblBacteriaiAAZ35196; AAZ35196; PSPPH_4399.
    GeneIDi3557772.
    KEGGipsp:PSPPH_4399.
    PATRICi19978289. VBIPseSyr78478_4601.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000058 Genomic DNA. Translation: AAZ35196.1 .
    RefSeqi YP_276515.1. NC_005773.3.

    3D structure databases

    ProteinModelPortali Q48DM6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 264730.PSPPH_4399.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ35196 ; AAZ35196 ; PSPPH_4399 .
    GeneIDi 3557772.
    KEGGi psp:PSPPH_4399.
    PATRICi 19978289. VBIPseSyr78478_4601.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi HPHIPTK.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci PSAV264730:GKDE-4403-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1448A / Race 6.

    Entry informationi

    Entry nameiLIPA_PSE14
    AccessioniPrimary (citable) accession number: Q48DM6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3