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Q48DM6

- LIPA_PSE14

UniProt

Q48DM6 - LIPA_PSE14

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Protein
Lipoyl synthase
Gene
lipA, PSPPH_4399
Organism
Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi81 – 811Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi92 – 921Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi107 – 1071Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi111 – 1111Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi114 – 1141Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPSAV264730:GKDE-4403-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:PSPPH_4399
OrganismiPseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)
Taxonomic identifieri264730 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000551: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Lipoyl synthaseUniRule annotation
PRO_0000325292Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264730.PSPPH_4399.

Structurei

3D structure databases

ProteinModelPortaliQ48DM6.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q48DM6-1 [UniParc]FASTAAdd to Basket

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MTDTVQTLIP TLDVSERVAR PKVEAGVKLR GAEKVARIPV KIIPTVDLPK    50
KPDWIRVRIP VSPEVDRIKQ LLRKHKLHSV CEEASCPNLG ECFSGGTATF 100
MIMGDICTRR CPFCDVGHGR PKPLDVNEPK SLAIAIADLR LKYVVITSVD 150
RDDLRDGGAQ HFADCIREIR LLSPGVQLET LVPDYRGRMD VALEITAAEP 200
PDVFNHNLET VPRLYKAARP GSDYQWSLTL LQRFKQMVPH VPTKSGLMLG 250
LGETDEEVIE VMQRMREHDI DMLTLGQYLQ PSRNHLPVQR FVHPDTFAWF 300
AEEGYKMGFK NVASGPLVRS SYHADEQAKI AKAML 335
Length:335
Mass (Da):37,665
Last modified:September 13, 2005 - v1
Checksum:i198B58FE2D1E004A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000058 Genomic DNA. Translation: AAZ35196.1.
RefSeqiYP_276515.1. NC_005773.3.

Genome annotation databases

EnsemblBacteriaiAAZ35196; AAZ35196; PSPPH_4399.
GeneIDi3557772.
KEGGipsp:PSPPH_4399.
PATRICi19978289. VBIPseSyr78478_4601.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000058 Genomic DNA. Translation: AAZ35196.1 .
RefSeqi YP_276515.1. NC_005773.3.

3D structure databases

ProteinModelPortali Q48DM6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264730.PSPPH_4399.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ35196 ; AAZ35196 ; PSPPH_4399 .
GeneIDi 3557772.
KEGGi psp:PSPPH_4399.
PATRICi 19978289. VBIPseSyr78478_4601.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci PSAV264730:GKDE-4403-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1448A / Race 6.

Entry informationi

Entry nameiLIPA_PSE14
AccessioniPrimary (citable) accession number: Q48DM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: September 13, 2005
Last modified: May 14, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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