Reviewed, UniProtKB/Swiss-Prot Q48BL6 (PDXY_PSE14)

Names and origin

Protein names

Recommended name:
    Pyridoxamine kinase
      Short name=PM kinase
    EC=2.7.1.35

Gene names

Name:	pdxY
Ordered Locus Names:	PSPPH_5152

Organism

Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) [Complete proteome] [HAMAP]

Taxonomic identifier

264730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxamine By similarity.
Catalytic activity	ATP + pyridoxal = ADP + pyridoxal 5'-phosphate. HAMAP MF_01639
Sequence similarities	Belongs to the pyridoxine kinase family.

Ontologies

Keywords
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glucokinase activity Inferred from electronic annotation. Source: HAMAP pyridoxal kinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Pyridoxamine kinase HAMAP MF_01639		PRO_0000269822
Regions
	Nucleotide binding	184 – 185	2	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	47	1	Substrate By similarity
	Binding site	225	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48BL6-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 67295C04ED48F0F7	FASTA	288	31,012
	10 20 30 40 50 60 MKRTPHLLAI QSHVVFGHAG NSAAVFPMQR IGVNVWPLNT VQFSNHTQYK QWTGEVLAPQ 70 80 90 100 110 120 QIPALIEGIA AIGELGNCDA VLSGYLGSAA QGRAILTGVA RIKAANPKAL YLCDPVMGHP 130 140 150 160 170 180 EKGCIVAPEV SDFLLQEAAA MADFMCPNQL ELDSFSGRKP ESLADCLAMA RALLARGPKA 190 200 210 220 230 240 VVVKHLDYPG KAADGFEMLL VTAEASWHLR RPLLAFPRQP VGVGDLTSGL FLSRILLGDD 250 260 270 280 LVAAFEFTAA AVHEVLLETQ ACGSYELELV RAQDRIAHPR VKFDAVRL

References

[1]

"Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition." Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J., Creasy T., Davidsen T.M., Haft D.H. , Zafar N., Zhou L., Halpin R., Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M., Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A., Buell R. J. Bacteriol. 187:6488-6498(2005) [PubMed: 16159782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000058 Genomic DNA. Translation: AAZ36132.1.
RefSeq	YP_277229.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3558742.
GenomeReviews	Gene locus PSPPH_5152 in contig CP000058_GR.
KEGG	psp:PSPPH_5152.
NMPDR	fig|264730.3.peg.5169.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q48BL6.
OMA	Q48BL6. EVLLETQ.
Family and domain databases
HAMAP	MF_01639. [Tree]
InterPro	IPR011611. Carb/pur_kinase. IPR004625. PyrdxlP_synth_PyrdxlKinase. [Graphical view]
Pfam	PF00294. PfkB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00687. pyridox_kin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PDXY_PSE14

Accession

Primary (citable) accession number: Q48BL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)