Reviewed, UniProtKB/Swiss-Prot Q48AV5 (AROE_COLP3)

Names and origin

Protein names

Recommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	CPS_0030

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Shikimate + NADP(+) = 3-dehydroshikimate + NADPH.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 4/7.
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Shikimate dehydrogenase		PRO_0000325113
Regions
	Nucleotide binding	130 – 134	5	NADP By similarity
Sites
	Active site	70	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q48AV5-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: E015664A84C75F12	FASTA	275	29,785
	10 20 30 40 50 60 MTQQTPDQYR VFGNPIEQSK SPAIHHIFAD KSQQNIDYQK QLVDTKDFSN AVADFIRHGG 70 80 90 100 110 120 KGANVTAPFK EQALAIADEL TERATLAGAV NTLTFKNGKI FGDNTDGEGL VQDLITNKVI 130 140 150 160 170 180 LNESRVLLLG AGGAARGVLL PLLAQNPRSI VIANRTASKA ATLCQHFSDI RLSASGYQDL 190 200 210 220 230 240 EQQHFDVIIN ATSASLSGNL PPIPTSLLSQ NVVCYDMVYG KDETPFLKWA KEHGAMKVID 250 260 270 GLGMLVGQAA VSFEVWRGVT PEVQPVIDKL RASLK

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000083 Genomic DNA. Translation: AAZ27050.1.
RefSeq	YP_266812.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3521306.
GenomeReviews	Gene locus CPS_0030 in contig CP000083_GR.
KEGG	cps:CPS_0030.
NMPDR	fig|167879.3.peg.47.
TIGR	CPS_0030.
Phylogenomic databases
HOGENOM	Q48AV5.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_0030-MON.
Family and domain databases
HAMAP	MF_00222. [Tree]
InterPro	IPR016040. NAD(P)-bd. IPR011342. Quinate/shikimate_5-DHase. IPR013708. Shikimate_DHase-bd_N. IPR006151. Shikm_DHase/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. aroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_COLP3

Accession

Primary (citable) accession number: Q48AV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	September 13, 2005
Last modified:	November 25, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)