Reviewed, UniProtKB/Swiss-Prot Q48AR8 (DAPF_COLP3)

Names and origin

Protein names

Recommended name:
    Diaminopimelate epimerase
      Short name=DAP epimerase
    EC=5.1.1.7

Gene names

Name:	dapF
Ordered Locus Names:	CPS_0077

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate. HAMAP MF_00197
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-diaminopimelate from LL-diaminopimelate: step 1/1. HAMAP MF_00197
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the diaminopimelate epimerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysine biosynthetic process via diaminopimelate Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	diaminopimelate epimerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	Diaminopimelate epimerase HAMAP MF_00197		PRO_1000011871
Sites
	Active site	75	1	By similarity
	Active site	219	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48AR8-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: AD258120101B30AC	FASTA	276	30,230
	10 20 30 40 50 60 MLVNFSKMHG LGNDFLVLDN VTQNVFLSPE QITKFANRNF GVGFDQLLVV EPPYDPDLDF 70 80 90 100 110 120 HYRIYNADGS EVGQCGNGAR CFAKFVRMKG LCNKHKIKVS TSTGKMNLHI ERDGNISVTM 130 140 150 160 170 180 PVPQFEPKKI PFTAQKTEGT YILRSESETV LCGAVSMGNP HCVVTVDSVA EADVESLGKE 190 200 210 220 230 240 LSVHERFPED ANVGFMEIVS PNYIKLRVYE RGAAETLACG SGACAAVVIG YMQKKLAKQV 250 260 270 TVELPGGKLR IFWQGPGHPV KMSGPATHVF DGQISI

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000083 Genomic DNA. Translation: AAZ24959.1.
RefSeq	YP_266845.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3518496.
GenomeReviews	Gene locus CPS_0077 in contig CP000083_GR.
KEGG	cps:CPS_0077.
NMPDR	fig|167879.3.peg.80.
TIGR	CPS_0077.
Phylogenomic databases
HOGENOM	Q48AR8.
OMA	Q48AR8. PHAVLRV.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_0077-MON.
Family and domain databases
HAMAP	MF_00197. [Tree]
InterPro	IPR001653. DAP_epimerase. IPR018510. DAP_epimerase_CS. [Graphical view]
Pfam	PF01678. DAP_epimerase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00652. DapF. 1 hit.
PROSITE	PS01326. DAP_EPIMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPF_COLP3

Accession

Primary (citable) accession number: Q48AR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)