Reviewed, UniProtKB/Swiss-Prot Q48AN3 (RNPH_COLP3)

Names and origin

Protein names

Recommended name:
    Ribonuclease PH
      Short name=RNase PH
    EC=2.7.7.56
Alternative name(s):
    tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	CPS_0112

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity.
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 237	237	Ribonuclease PH HAMAP MF_00564		PRO_1000024798

Sequences

	Sequence		Length	Mass (Da)
	Q48AN3-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 3541AD3F3B5E510D	FASTA	237	25,588
	10 20 30 40 50 60 MRPSGRTLGQ IRPVTITRQF TTHAEGSVLI EFGDTKVICT ATVEVGVPRF LKGQGKGWVT 70 80 90 100 110 120 AEYGMLPRST HTRMRREAAS GKQSGRTLEI SRLIARALRA AVDLKALGEN TISVDCDVIQ 130 140 150 160 170 180 ADGGTRTAAI TGACVALVDA LNYMRAKDII KTNPLKHMIA AVSVGIYKGE PVADLDYPED 190 200 210 220 230 SAADTDMNVV MTDTGKLIEV QGTAEEEPFS FEEMQAMLEL AKNGINELFD LQKAALS

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000083 Genomic DNA. Translation: AAZ24239.1.
RefSeq	YP_266880.1.
3D structure databases
SMR	Q48AN3. Positions 2-237.
ModBase	Search...
Genome annotation databases
GeneID	3519509.
GenomeReviews	Gene locus CPS_0112 in contig CP000083_GR.
KEGG	cps:CPS_0112.
NMPDR	fig|167879.3.peg.114.
TIGR	CPS_0112.
Phylogenomic databases
HOGENOM	Q48AN3.
OMA	Q48AN3. YAMLPRA.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_0112-MON.
Family and domain databases
HAMAP	MF_00564. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR018336. Ribonuclease-PH_CS. IPR002381. RNase_PH_bac-type. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_COLP3

Accession

Primary (citable) accession number: Q48AN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)