Reviewed, UniProtKB/Swiss-Prot Q48AL2 (PLSB_COLP3)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	CPS_0133

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	811 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 811	811	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049429
Regions
	Motif	309 – 314	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	Q48AL2-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 8D4D8AA84F1B673B	FASTA	811	91,530
	10 20 30 40 50 60 MLALRSFFYL LLKFPLKLLV RCKIITDSQN ITDQPNQPIF YIVRHQSASD LLALQSACKK 70 80 90 100 110 120 QNLPDPLGKV TINGESFNRT LCLAKSTPLC SWRKSSKTTA TAQGLALLNQ HVIDENIDAK 130 140 150 160 170 180 LIPANLIWGR TPTKERKNLN IGTLLADQES PNWLRKFFIV LFLGRDTLVR FSEAFSLRNI 190 200 210 220 230 240 SDNHGSDEAA AHKFLRVARF HFHRQTIAAK GPRLMHRKQM FTALFANPSV KRIISDEAKN 250 260 270 280 290 300 KKVSEAEIKK KALVMMNEIA GDYSVSWLRF GEIILHWLWK RLYSAIKVSN AKVLRKLAQD 310 320 330 340 350 360 GHEIIYVPCH RSHMDYLLLS YVILQEGLVM PRIAAGINLN FWPAGTIFRK GGAFFIRRSF 370 380 390 400 410 420 GGNRLYSTIF REYLGLLFER GYGVKYYTEG GRSRTGRVLA PKTGMLAMTI QSLLRGIDRP 430 440 450 460 470 480 LTLVPVYLGY EHVMEVGTYH KELSGSEKKG ESMFGVLKAI KSLRNYGNGY VNFGEPMNIN 490 500 510 520 530 540 EFLNKQVPDW KDSIDPIDPQ KPSWLTPTVN VLADQVMENI NKSAALNGVA LIALILHASK 550 560 570 580 590 600 NKALSKLELE TQLDFFLNIQ RQAPFSEQLT IPEETGAELL THVISLNKVT ITEDSFGSLV 610 620 630 640 650 660 SLSETANTEM RYYRNNILHT YVVPALVCRL LDKHSKINQD ELVIKVQNVT ALLKEDLYLY 670 680 690 700 710 720 QDSTHVEQQT LRVLTVLKEM EIAKQTKAGF WSLSDDVGLL SQVHAMAECI DESLQRLAII 730 740 750 760 770 780 TSLTSRLAPL SKRDLETKVV AIAKRLSVLN NINAPEFIDK RAQSTLIATI REQGYIDLDD 790 800 810 DGLLIASSTM AEIKATVINL VDIEVLQSIA R

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000083 Genomic DNA. Translation: AAZ27641.1.
RefSeq	YP_266901.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q48AL2.
Genome annotation databases
GeneID	3521900.
GenomeReviews	Gene locus CPS_0133 in contig CP000083_GR.
KEGG	cps:CPS_0133.
NMPDR	fig|167879.3.peg.135.
TIGR	CPS_0133.
Phylogenomic databases
HOGENOM	Q48AL2.
OMA	VPVYIGY.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_0133-MON.
Family and domain databases
HAMAP	MF_00393. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_COLP3

Accession

Primary (citable) accession number: Q48AL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)