Q48A14 (STHA_COLP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Soluble pyridine nucleotide transhydrogenase
Short name=STH
EC=1.6.1.1

Alternative name(s):
NAD(P)(+) transhydrogenase [B-specific]

Gene names

Name:	sthA
Ordered Locus Names:	CPS_0334

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation By similarity. HAMAP MF_00247
Catalytic activity	NADPH + NAD+ = NADP+ + NADH. HAMAP MF_00247
Cofactor	Binds 1 FAD per subunit By similarity. HAMAP MF_00247
Subcellular location	Cytoplasm By similarity HAMAP MF_00247.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD(P)+ transhydrogenase (B-specific) activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Soluble pyridine nucleotide transhydrogenase HAMAP MF_00247		PRO_0000260232
Regions
	Nucleotide binding	36 – 45	10	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48A14 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: F82A52806BF28019	FASTA	466	51,181
	10 20 30 40 50 60 MEKSFDYDVI IIGTGPGGEG AAMNLAKRQK KVAIIERYHQ VGGGCTHWGT IPSKALRQSV 70 80 90 100 110 120 SRLIEYNSNP LFNQNEQVKQ LTFQDILSHA SAVIQKQVSL RSGFYNRNRV EHIQGQASFI 130 140 150 160 170 180 DAHTISISHP DGSVEKISAK QIMIATGSRP YRPDDIDFDH PRVYDSDSIL SLKHAPQHVI 190 200 210 220 230 240 IYGAGVIGSE YASIFRGLGV KVDLINTRER LLSFLDTEMS DSLSYHLWNS GVVIRHGEEI 250 260 270 280 290 300 ERVESSEDAV IVHLKSGKKM RADCLLFANG RTGNTADLNL AAAGLKADGR GQLKVNDCYQ 310 320 330 340 350 360 TEVDNIFAVG DVIGYPSLAS AAFDQGRIAA SAMVDSSSKA KLIVDIPTGI YTIPEISSVG 370 380 390 400 410 420 KTEQELTEAK IPYEVGRAQF KHLARAQISN NLVGSLKILF HRETKEILGI HCFGENAAEI 430 440 450 460 IHIGQAIMQQ TNGGNTIEYF VETTFNYPTM AEAFRVAALN GLNRLF

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 34H / ATCC BAA-681.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000083 Genomic DNA. Translation: AAZ24633.1.
RefSeq	YP_267100.1. NC_003910.7.
3D structure databases
HSSP	HSSP built from PDB template 2F5Z based on UniProtKB P09622.
ProteinModelPortal	Q48A14.
ModBase	Search...
Protein-protein interaction databases
STRING	Q48A14.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3518988.
GenomeReviews	Gene locus CPS_0334 in contig CP000083_GR.
KEGG	cps:CPS_0334.
NMPDR	fig|167879.3.peg.192.
PATRIC	21464055. VBIColPsy94388_0298.
TIGR	CPS_0334.
Phylogenomic databases
eggNOG	COG1249.
HOGENOM	HBG515043.
OMA	GEGNTIE.
ProtClustDB	PRK05249.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_0334-MONOMER.
Family and domain databases
HAMAP	MF_00247. SthA. [Tree]
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR001327. Pyr_OxRdtase_NAD-bd_dom. IPR022962. STH. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
KO	K00322.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

STHA_COLP3

Accession

Primary (citable) accession number: Q48A14

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program