Q489W4 (FADA_COLP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-ketoacyl-CoA thiolase
EC=2.3.1.16

Alternative name(s):
Acetyl-CoA acyltransferase
Beta-ketothiolase
Fatty acid oxidation complex subunit beta

Gene names

Name:	fadA
Ordered Locus Names:	CPS_0392

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed By similarity. HAMAP MF_01620
Catalytic activity	Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA. HAMAP MF_01620
Pathway	Lipid metabolism; fatty acid beta-oxidation. HAMAP MF_01620
Subunit structure	Heterotetramer of two alpha chains (fadB) and two beta chains (fadA) By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01620.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid degradation Lipid metabolism
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	3-ketoacyl-CoA thiolase HAMAP MF_01620		PRO_0000206369
Sites
	Active site	91	1	Acyl-thioester intermediate By similarity
	Active site	342	1	Proton acceptor By similarity
	Active site	372	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q489W4 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: E190343259167702	FASTA	386	40,645
	10 20 30 40 50 60 MNEVVIVDCI RTPMGRSKAG VFRNVRAEAL SAHLMKQILV RNPALNPEDI EDVIWGCVKQ 70 80 90 100 110 120 TKEQGFNIAR NASLLAGLPK SIGGVTVNRL CGSSMEALHQ ASTSIMSGQG DVFLIGGVEH 130 140 150 160 170 180 MGHVPMMYDV DFDPALNKNI ALASGNMGLT AELLGKQHGI TREMQDAFGA RSHQKAHEAH 190 200 210 220 230 240 LAGRWDNEIV ATQGHDATGA LTLVEHDEVI RPETTAESLS ALRPVFDPVN GTVTAGTSSA 250 260 270 280 290 300 LSDGASAMLV MSAAKAKELG LTPRVKIRGM AVAGCDPATM GFGPVPATKK ALKRAGLSIA 310 320 330 340 350 360 DIELFEFNEA FAAQALSCVR SLKVEDKMDQ INLNGGAIAL GHPLGCSGSR ISGTLINLME 370 380 GQDVNIGLAT MCIGLGQGIA TVFERV

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 34H / ATCC BAA-681.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000083 Genomic DNA. Translation: AAZ25024.1.
RefSeq	YP_267150.1. NC_003910.7.
3D structure databases
ProteinModelPortal	Q489W4.
SMR	Q489W4. Positions 3-386.
ModBase	Search...
Protein-protein interaction databases
STRING	Q489W4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3518581.
GenomeReviews	Gene locus CPS_0392 in contig CP000083_GR.
KEGG	cps:CPS_0392.
NMPDR	fig|167879.3.peg.450.
PATRIC	21464149. VBIColPsy94388_0339.
TIGR	CPS_0392.
Phylogenomic databases
eggNOG	COG0183.
HOGENOM	HBG370930.
OMA	QDAFGAR.
ProtClustDB	PRK08947.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_0392-MONOMER.
Family and domain databases
HAMAP	MF_01620. FadA. [Tree]
InterPro	IPR012805. FadA. IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 4 hits.
KO	K00632.
PANTHER	PTHR18919:SF35. PTHR18919:SF35. 1 hit. PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit. TIGR02445. FadA. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FADA_COLP3

Accession

Primary (citable) accession number: Q489W4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program