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Q489P3 (ASSY_COLP3) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:CPS_0463
OrganismColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]
Taxonomic identifier167879 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia

Protein attributes

Sequence length418 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 418418Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_0000263915

Regions

Nucleotide binding14 – 229ATP By similarity

Sites

Binding site421ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site941Citrulline By similarity
Binding site991Citrulline By similarity
Binding site1241ATP; via amide nitrogen By similarity
Binding site1261Aspartate By similarity
Binding site1301Aspartate By similarity
Binding site1301Citrulline By similarity
Binding site1311Aspartate By similarity
Binding site1341Citrulline By similarity
Binding site1831Citrulline By similarity
Binding site1921Citrulline By similarity
Binding site2731Citrulline By similarity
Binding site2851Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
Q489P3 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: BC6740EAA891B1E8

FASTA41845,950
        10         20         30         40         50         60 
MALAKKTIKK VVLAYSGGLD TSAIIPWLKE NYDGCEVIAF CADVGQGDEE LEGVKEKAIA 

        70         80         90        100        110        120 
SGASECYVVD LKEEYVKEYI YPILKTGSVY EGQYLLGTSM ARPIIAKAHI EVALKVGADA 

       130        140        150        160        170        180 
VCHGCTGKGN DQVRFESCFA ALAPELTVIA PWREWDMVSR EDLLDYLAER NIPCAASLTK 

       190        200        210        220        230        240 
IYSRDANAWH ISHEGGELED PWCEPSKEVW TMTVDPMDAP DVPEKVQLSF KEGELVGIDG 

       250        260        270        280        290        300 
KDFSQHGAGA YEALMYLNEK GSAHGVGRID IVENRLVGMK SRGCYETPGG TILMAAYKGL 

       310        320        330        340        350        360 
ETLILDKESL KYRESVGLEF SHVIYDGRWF TPLAKAQLAS AASFAEKLTG DVVVKLYKGM 

       370        380        390        400        410 
AQVIQRRSPN SLYSEAFATF GADDVYDQKH AEGFIRLFSL SSRITALSQK DSLKDKAE 

« Hide

References

[1]"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses."
Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. expand/collapse author list , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 34H / ATCC BAA-681.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000083 Genomic DNA. Translation: AAZ24202.1.
RefSeqYP_267221.1. NC_003910.7.

3D structure databases

ProteinModelPortalQ489P3.
SMRQ489P3. Positions 10-410.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING167879.CPS_0463.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ24202; AAZ24202; CPS_0463.
GeneID3519550.
KEGGcps:CPS_0463.
PATRIC21464279. VBIColPsy94388_0404.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAPWNEPTK.
OrthoDBEOG6K9QCV.

Enzyme and pathway databases

BioCycCPSY167879:GI48-463-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_COLP3
AccessionPrimary (citable) accession number: Q489P3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: September 13, 2005
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways