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Q489F4 (PUR9_COLP3) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

  1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
    EC=2.1.2.3
    Alternative name(s):
    AICAR transformylase
  2. IMP cyclohydrolase
    EC=3.5.4.10
    Alternative name(s):
    ATIC
    IMP synthase
    Inosinicase
Gene names
Name:purH
Ordered Locus Names:CPS_0552
OrganismColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]
Taxonomic identifier167879 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP-Rule MF_00139

Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.

Domain

The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP-Rule MF_00139

Sequence similarities

Belongs to the PurH family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 533533Bifunctional purine biosynthesis protein PurH HAMAP-Rule MF_00139
PRO_1000018883

Sequences

Sequence LengthMass (Da)Tools
Q489F4 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 9D91E97F3A8FF883

FASTA53357,793
        10         20         30         40         50         60 
MDTPRPIKRA LLSVSDKAGI VEFAQRLSEK GVDLLSTGGT AKLLAENGIK VTEVSDYTGH 

        70         80         90        100        110        120 
PEIMDGRVKT LHPKVHGGIL ARRGIDEVVM DENGISAIDM VVVNLYPFAN AVSDENCSLE 

       130        140        150        160        170        180 
NAIENIDIGG PTMVRAAAKN HKDVTIVVNA SDYERVLTEL DNNNDSLTYK TRFDLAIAAY 

       190        200        210        220        230        240 
EHTASYDGMI ANYFGKMLPA YGETESKASL ENKVKFPRTF NSQFIKTQDL RYGENSHQDA 

       250        260        270        280        290        300 
AFYKEENPEE ASVSTATQLQ GKALSYNNIA DTDAALECVK EFDEPACVIV KHANPCGVAI 

       310        320        330        340        350        360 
GDDILAAYEG AYKTDPTSAF GGIIAFNREL DADTAEAIVS RQFVEVIIAP SVSDAAAQIV 

       370        380        390        400        410        420 
AAKPNLRLLE CGQWDNKTTG FDFKRVNGGL LVQDTDQGRV TSDDLTVVTK RQPTDEEMRD 

       430        440        450        460        470        480 
LQFCWKVAKF VKSNAIVYVK NSSTIGVGAG QMSRVYSAKV AGIKAADENL EVKGSVMASD 

       490        500        510        520        530 
AFFPFRDGLD AAAEAGITAV IQPGGSMRDD EVIAAADEHN IAMVFTGMRH FRH 

« Hide

References

[1]"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses."
Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. expand/collapse author list , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 34H / ATCC BAA-681.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000083 Genomic DNA. Translation: AAZ28762.1.
RefSeqYP_267310.1. NC_003910.7.

3D structure databases

ProteinModelPortalQ489F4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING167879.CPS_0552.

Proteomic databases

PRIDEQ489F4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ28762; AAZ28762; CPS_0552.
GeneID3523024.
KEGGcps:CPS_0552.
PATRIC21464443. VBIColPsy94388_0486.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0138.
HOGENOMHOG000230373.
KOK00602.
OMADLLFAWK.
OrthoDBEOG6QCDFF.
ProtClustDBPRK00881.

Enzyme and pathway databases

BioCycCPSY167879:GI48-552-MONOMER.
UniPathwayUPA00074; UER00133.
UPA00074; UER00135.

Family and domain databases

Gene3D3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPMF_00139. PurH.
InterProIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERPTHR11692. PTHR11692. 1 hit.
PfamPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsTIGR00355. purH. 1 hit.
ProtoNetSearch...

Entry information

Entry namePUR9_COLP3
AccessionPrimary (citable) accession number: Q489F4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: February 19, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways