Reviewed, UniProtKB/Swiss-Prot Q488M6 (SYV_COLP3)

Names and origin

Protein names

Recommended name:
    Valyl-tRNA synthetase
    EC=6.1.1.9
Alternative name(s):
    Valine--tRNA ligase
      Short name=ValRS

Gene names

Name:	valS
Ordered Locus Names:	CPS_0738

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	973 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity.
Catalytic activity	ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP MF_02004
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	valyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP valine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 973	973	Valyl-tRNA synthetase HAMAP MF_02004		PRO_0000224464
Regions
	Coiled coil	901 – 970	70	Potential
	Motif	57 – 67	11	"HIGH" region HAMAP MF_02004
	Motif	569 – 573	5	"KMSKS" region HAMAP MF_02004
Sites
	Binding site	572	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q488M6-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: F2650EE687C39838	FASTA	973	110,937
	10 20 30 40 50 60 MLQQNNEQLL NLLPDKMMEK TFNPTDIEQS LYTSWEEQGY FSPTGEGDSY SIAIPPPNVT 70 80 90 100 110 120 GSLHMGHAFQ QTIMDTLIRY QRMQGKNTLW QTGCDHAGIA TQMVVERKIA AEEDKTRHDY 130 140 150 160 170 180 GREGFIDKIW EWKEESGGTI GKQMRRLGNS IDWSRERFTM DDGMSEAVQE VFVRLFEDDL 190 200 210 220 230 240 IYRGKRLVNW DPKFHTAISD LEVENKDKKG HMWHLRYPLA NGAKTAEGLD YLVVATTRPE 250 260 270 280 290 300 TMLGDTGVAV NPEDPRYKDL IGKQVLLPLV NRLIPIVGDD HADMEKGTGC VKITPGHDFN 310 320 330 340 350 360 DNEVGKRHAL PQINILDKDA AILATAEVYD TKGEVCNAYD TGLPSEFAGM DRFVARKAIV 370 380 390 400 410 420 AKFDELGLLV EVKDHDLVAP YGDRSGVIIE PLLTDQWYVR VEKLAGPAVD AVKDGQIEFV 430 440 450 460 470 480 PKQYENMYFS WMNNIQDWCI SRQLWWGHRI PAWYDENEKV YVGRTEEEVR ANNDIAADMK 490 500 510 520 530 540 LRQDDDVLDT WFSSALWTFS TLGWPKDTED LKTFHPTDVL VTGFDIIFFW VARMIMMTMH 550 560 570 580 590 600 FNKDENGKAQ IPFKKIYMTG LIRDENGDKM SKSKGNVVDP LDMIDGISLE DLLQKRTGNM 610 620 630 640 650 660 MQPKLAKKIE KLTRKEYPEG IEAHGTDALR FTLTSVATTG RDISWDMKRL EGYRNFTNKL 670 680 690 700 710 720 WNASRYVMMN TEEFDCGQSS PEGKAGDMEL SLADRWIIGQ FEQTVKTVHE AFDTYRFDLA 730 740 750 760 770 780 SQALYEFTWN QFCDWYLELT KPVLFKENEA QQRGTRHTLV NVLEALLRLM HPIMPFITET 790 800 810 820 830 840 IWQRVQPLSD FSKNGDSIMV QAFPQFDESK CDQQAIDDLE WVKQFIIAIR NIRGEMDISP 850 860 870 880 890 900 SKELPVLLKN VNDNDQRRLD ENEQFLSSLA KLESITVLAD DEQGPASASA VVGDLSVLIP 910 920 930 940 950 960 MAGLIDKEAE LARLDKAIEK LEKEAGRVRG KLGNENFVSK APAAVIEKEQ AKLADAESTL 970 AKILEQKIQI AAL

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000083 Genomic DNA. Translation: AAZ27693.1.
RefSeq	YP_267488.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3521952.
GenomeReviews	Gene locus CPS_0738 in contig CP000083_GR.
KEGG	cps:CPS_0738.
NMPDR	fig|167879.3.peg.512.
TIGR	CPS_0738.
Phylogenomic databases
HOGENOM	Q488M6.
OMA	Q488M6. TDQWYVS.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_0738-MON.
Family and domain databases
HAMAP	MF_02004. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR002303. Val-tRNA_synth_Ia. IPR011321. Val-tRNA_synth_Ia_C. IPR019754. Val-tRNA_synth_Ia_N. IPR019499. Val-tRNA_synth_Ia_tRNA-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.287.380. Val-tRNA_synth_Ia_C. 1 hit.
PANTHER	PTHR11946:SF5. tRNA-synt_val. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF10458. Val_tRNA-synt_C. 1 hit. [Graphical view]
PRINTS	PR00986. TRNASYNTHVAL.
TIGRFAMs	TIGR00422. valS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYV_COLP3

Accession

Primary (citable) accession number: Q488M6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)