Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q48846 (BGAL_LACSK) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase large subunit

Short name=Beta-gal large subunit
EC=3.2.1.23
Alternative name(s):
Lactase large subunit
Gene names
Name:lacL
OrganismLactobacillus sakei
Taxonomic identifier1599 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length625 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subunit structure

Heterodimer of a large and a small subunit.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 625625Beta-galactosidase large subunit
PRO_0000057670

Sites

Active site4651Proton donor By similarity
Active site5331Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q48846 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C658A3C46136B886

FASTA62572,457
        10         20         30         40         50         60 
MQPNIQWLDT PAVFRVGQLP AHSDHRYYAT LAEMAQQQSS FEQSLNGTWQ FHYSVNAASR 

        70         80         90        100        110        120 
PKSFYELAFD AQDFEPITVP QHIELAGYEQ LHYINTMYPW EGHYYRRPAF STSDDKQHLG 

       130        140        150        160        170        180 
MFSEADYNPV GSYLHHFDLT PALRNQRVII RFEGVEQAMY VWLNGQFIGY AEDSFTPSEF 

       190        200        210        220        230        240 
DLTPYLKETD NCLAVEVHKR SSAAFIEDQD FFRFFGIFRD VKLLAKPRTH LEDLWVIPEY 

       250        260        270        280        290        300 
DVVQQTGQVK LRLQFSGDEN RVHLRIRDQH QIILTADLTS GAQVNDLYKM PELVQAWSNQ 

       310        320        330        340        350        360 
TPNLYTLELE VVDQAGETIE ISQQPFGFRK IEIKDKVMLL NGKRLVINGV NRHEWHPETG 

       370        380        390        400        410        420 
RTITAEDEAW DIACMQRNHI NAVRTSHYPD RLSFYNGCDQ AGIYMMAETN LESHGSWQKM 

       430        440        450        460        470        480 
GAVEPSWNVP GSYDEWEAAT LDRARTNFET FKNHVSILFW SLGNESYAGS VLEKMNAYYK 

       490        500        510        520        530        540 
QQDPTRLVHY EGVFRAPEYK ATISDVESRM YATPAEIKAY LDNAPQKPFI LCEYMHDMGN 

       550        560        570        580        590        600 
SLGGMQSYID LLSQYDMYQG GFIWDFIDQA LLVTDPVTGQ RELRYGGDFD DRPSDYEFSG 

       610        620 
DGLVFATRDE KPAMQEVRYY YGEHK 

« Hide

References

[1]"Two genes encoding the beta-galactosidase of Lactobacillus sake."
Obst M., Meding E.R., Vogel R.F., Hammes W.P.
Microbiology 141:3059-3066(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 20017.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X82287 Genomic DNA. Translation: CAA57730.1.

3D structure databases

ProteinModelPortalQ48846.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SUPFAMSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_LACSK
AccessionPrimary (citable) accession number: Q48846
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: October 16, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries