Q48846 (BGAL_LACSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase large subunit
Short name=Beta-gal large subunit
EC=3.2.1.23

Alternative name(s):
Lactase large subunit

Gene names

Name:

lacL

Organism

Lactobacillus sakei

Taxonomic identifier

1599 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subunit structure	Heterodimer of a large and a small subunit.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 625	625	Beta-galactosidase large subunit		PRO_0000057670
Sites
	Active site	465	1	Proton donor By similarity
	Active site	533	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48846 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C658A3C46136B886	FASTA	625	72,457
	10 20 30 40 50 60 MQPNIQWLDT PAVFRVGQLP AHSDHRYYAT LAEMAQQQSS FEQSLNGTWQ FHYSVNAASR 70 80 90 100 110 120 PKSFYELAFD AQDFEPITVP QHIELAGYEQ LHYINTMYPW EGHYYRRPAF STSDDKQHLG 130 140 150 160 170 180 MFSEADYNPV GSYLHHFDLT PALRNQRVII RFEGVEQAMY VWLNGQFIGY AEDSFTPSEF 190 200 210 220 230 240 DLTPYLKETD NCLAVEVHKR SSAAFIEDQD FFRFFGIFRD VKLLAKPRTH LEDLWVIPEY 250 260 270 280 290 300 DVVQQTGQVK LRLQFSGDEN RVHLRIRDQH QIILTADLTS GAQVNDLYKM PELVQAWSNQ 310 320 330 340 350 360 TPNLYTLELE VVDQAGETIE ISQQPFGFRK IEIKDKVMLL NGKRLVINGV NRHEWHPETG 370 380 390 400 410 420 RTITAEDEAW DIACMQRNHI NAVRTSHYPD RLSFYNGCDQ AGIYMMAETN LESHGSWQKM 430 440 450 460 470 480 GAVEPSWNVP GSYDEWEAAT LDRARTNFET FKNHVSILFW SLGNESYAGS VLEKMNAYYK 490 500 510 520 530 540 QQDPTRLVHY EGVFRAPEYK ATISDVESRM YATPAEIKAY LDNAPQKPFI LCEYMHDMGN 550 560 570 580 590 600 SLGGMQSYID LLSQYDMYQG GFIWDFIDQA LLVTDPVTGQ RELRYGGDFD DRPSDYEFSG 610 620 DGLVFATRDE KPAMQEVRYY YGEHK

References

[1]	"Two genes encoding the beta-galactosidase of Lactobacillus sake." Obst M., Meding E.R., Vogel R.F., Hammes W.P. Microbiology 141:3059-3066(1995) [PubMed: 8574399] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 20017.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X82287 Genomic DNA. Translation: CAA57730.1.
3D structure databases
ProteinModelPortal	Q48846.
ModBase	Search...
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR023232. Glyco_hydro_2_AS. IPR023230. Glyco_hydro_2_CS. IPR006102. Glyco_hydro_2_Ig-like. IPR006104. Glyco_hydro_2_N. IPR006103. Glyco_hydro_2_TIM. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF49303. Glyco_hydro_2Ig. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_LACSK

Accession

Primary (citable) accession number: Q48846

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 31, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program