Reviewed, UniProtKB/Swiss-Prot Q487V5 (HUTI_COLP3)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	CPS_0911

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity. HAMAP MF_00372
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential HAMAP MF_00372.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Imidazolonepropionase HAMAP MF_00372		PRO_0000306458
Sites
	Metal binding	79	1	Zinc or iron By similarity
	Metal binding	81	1	Zinc or iron By similarity
	Metal binding	249	1	Zinc or iron By similarity
	Metal binding	324	1	Zinc or iron By similarity
	Binding site	88	1	Substrate By similarity
	Binding site	101	1	Substrate By similarity
	Binding site	151	1	Substrate By similarity
	Binding site	184	1	Substrate By similarity
	Binding site	252	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q487V5-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: F56F59940883B432	FASTA	418	45,332
	10 20 30 40 50 60 MTLFKHWQTL YINVNLATMT DGSESYGEIS QGALAISAGK IAWLGKESDL PEHFSVTDED 70 80 90 100 110 120 IEVIDCKGQW LTPGLIDCHT HLVYGGNRAN EFEMRLQGKS YQEIANAGGG IVSTVTATRR 130 140 150 160 170 180 ASEQELLASA LPRLTALHQQ GVTTVEIKSG YGLDTINEIK MLKVAGLLAD ELPVTIKRTF 190 200 210 220 230 240 LGAHALPIEY KDNAEGYLDV VCEEMLPQVV SENLADAVDV FCEGIGFSLA QTKRVFDAAQ 250 260 270 280 290 300 SHDLPIKVHA EQLSNLGASE LAANYNALSS DHIEFLDEAG IKAMKKSGMT AVLLPGAFYF 310 320 330 340 350 360 LRETQLPPIE LLRKHQVPMA VATDANPGTS PIHNIHLMLN MACTLFRLTP SEALAGITCY 370 380 390 400 410 GAKALGLSES KGQLAVGYDA DIALWNINQP AELCYQFGVN PLSRLIQNGQ QVLMNESA

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000083 Genomic DNA. Translation: AAZ25600.1.
RefSeq	YP_267660.1.
3D structure databases
HSSP	HSSP built from PDB template 2GOK based on UniProtKB Q8U8Z6.
SMR	Q487V5. Positions 8-412.
ModBase	Search...
Protein-protein interaction databases
STRING	Q487V5.
Genome annotation databases
GeneID	3519842.
GenomeReviews	Gene locus CPS_0911 in contig CP000083_GR.
KEGG	cps:CPS_0911.
NMPDR	fig|167879.3.peg.878.
TIGR	CPS_0911.
Phylogenomic databases
eggNOG	COG1228.
HOGENOM	HBG686142.
OMA	MNMACTL.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_0911-MONOMER.
Family and domain databases
HAMAP	MF_00372. HutI. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_COLP3

Accession

Primary (citable) accession number: Q487V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	September 13, 2005
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)